It takes a little bit more than 100mg of cyanide to kill a 75 kg human being. A little more than 100mg and the unfortunate victim will show the telltale signs of “bluish tones of oxygen deprivation mottle the skin.“ Cyanide Poisoning is as old as the usage of chemical compounds containing cyanide and this episode looks at the chemistry that happens in the body during a poisoning. “This will be the last episode of Chemistry in Everyday Life for now. It was a huge amount of fun, but for the usual reasons, I have to suspend this beloved hobby project for an indefinite period of time. I would like to thank you all for listening. I enjoyed this experience immensely and I am proud of what I achieved. Take care everyone 😊” The Podcast will remain online for the time being, but I will not add any new content. Sources Historical Background · The Poisoner’s Handbook: Murder and the Birth of Forensic Medicine in Jazz Age New York, Chapter 3, 2010, D. Blum ISBN: 978-1594202438 Cyanide Poisoning and Cyanide Chemistry · https://en.wikipedia.org/wiki/Cyanide_poisoning · https://www.thoughtco.com/overview-of-cyanide-poison-609287 · https://en.wikipedia.org/wiki/Cyanide · https://en.wikipedia.org/wiki/Histotoxic_hypoxia Oxidative Phosphorylation and Cytochrome C Oxidase · https://en.wikipedia.org/wiki/Cytochrome_c_oxidase#Inhibition · https://en.wikipedia.org/wiki/Oxidative_phosphorylation · https://en.wikipedia.org/wiki/Electron_transport_chain Mitochondria · https://en.wikipedia.org/wiki/Mitochondrion · https://www.thoughtco.com/mitochondria-defined-373367 Medical Treatment for Cyanide Poisoning · https://www.drugs.com/cg/cyanide-poisoning.html · https://www.healthline.com/health/cyanide-poisoning

Welcome back to the last episode of this Nobel Prize series during which I discuss the technology for which the two research groups around Prof. emmanuelle Charpentier and Prof. Doudna received the joint Nobel Prize for chemistry. This episode will put all the pieces that we encountered over the course of the last two episodes together and finally will explain, how the so-called gene scissors, CRIPS/CAS9, work. I can now be reached on twitter under @ChemistryinEve1 , if you have feedback that you would like to share. Alternatively, you can send an email to chem.podcast@gmail.com. Sources · The original paper from 2012 discussing CIRSPR/CAS9 o https://pubmed.ncbi.nlm.nih.gov/22745249/ · Great articles outlining how CRISPR/CAS9 works o https://www.sciencenewsforstudents.org/article/explainer-how-crispr-works o https://sequencing.roche.com/en/blog/what-is-crispr-and-why-is-it-a-revolutionary-tool.html · Video explaining how CRISPR/CAS9 works o https://cen.acs.org/articles/98/web/2020/10/Video-CRISPR-Cas9-works.html · Wikipedia articles giving background information o https://en.wikipedia.org/wiki/Virus o https://de.wikipedia.org/wiki/CRISPR/Cas-Methode o https://en.wikipedia.org/wiki/CRISPR_gene_editing o https://en.wikipedia.org/wiki/Cas9 o https://de.wikipedia.org/wiki/Cas9 o https://en.wikipedia.org/wiki/Palindromic_sequence · Publications leading up to CRISPR/CAS9 o https://jb.asm.org/content/169/12/5429.short Paper mentioning CRISPR discovery o https://science.sciencemag.org/content/315/5819/1709 Paper mentioning CRISPR defense mechanism of bacteria · TED Talk by Prof. Doudna explaining her invention o https://www.youtube.com/watch?v=TdBAHexVYzc o Ethical discussion on how to use this technology o Remove HIV from human cells o Cure hereditary diseases · https://www.wilx.com/2020/10/07/nobel-peace-prize-awarded-for-gene-scissors/ o Link mentioning gene scissors · https://www.spiegel.de/wissenschaft/mensch/erbgut-vergleich-maus-und-mensch-sind-fast-identisch-a-198689.html