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Clinical Chemistry Podcast Profile

Clinical Chemistry Podcast

English, Chemistry, 1 season, 990 episodes, 5 days, 17 hours, 51 minutes
About
This free monthly podcast is offered by Clinical Chemistry. Clinical Chemistry is the leading forum for peer-reviewed, original research on innovative practices in today's clinical laboratory. In addition to being the most cited journal in the field, Clinical Chemistry has the highest Impact Factor (7.292 in 2019) among journals of clinical chemistry, clinical (or anatomic) pathology, analytical chemistry, and the subspecialties, such as transfusion medicine and clinical microbiology.
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Screening in Multiple Myeloma and Its Precursors: Are We There Yet?

2/2/202413 minutes, 3 seconds
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Eight Misconceptions about Prostate-Specific Antigen

1/19/202416 minutes, 14 seconds
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Cancer Screening, Incidental Detection, and Overdiagnosis

1/17/202412 minutes, 20 seconds
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Audio Summary February 2024

1/17/20246 minutes, 3 seconds
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Reevaluating the Role of High-Density Lipoprotein Cholesterol: New Perspectives on Cardiovascular Disease and Alzheimer Disease

1/2/20248 minutes, 50 seconds
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Data Analytics in Clinical Laboratories: Advancing Diagnostic Medicine in the Digital Age

1/2/202414 minutes, 12 seconds
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Audio Summary January 2024

12/18/202310 minutes, 22 seconds
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Sensitive Blood-Based Detection of HIV-1 and Mycobacterium tuberculosis Peptides for Disease Diagnosis by Immuno-Affinity Liquid Chromatography–Tandem Mass Spectrometry: A Method Development and Proof-of-Concept Study

12/1/20239 minutes, 13 seconds
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Audio Summary December 2023

11/14/20235 minutes, 42 seconds
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Early and Accurate Diagnosis of Parkinson Disease May Be Rooted in Seed Amplification Assays

11/7/202312 minutes, 8 seconds
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AI Chatbots in Clinical Laboratory Medicine: Foundations and Trends

11/7/202312 minutes, 4 seconds
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Generalizability of a Machine Learning Model for Improving Utilization of Parathyroid Hormone-Related Peptide Testing across Multiple Clinical Centers

10/30/202311 minutes, 50 seconds
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Audio Summary November 2023

10/18/20235 minutes, 17 seconds
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Confirmation of Insertion, Deletion, and Deletion-Insertion Variants Detected by Next-Generation Sequencing

10/16/202311 minutes, 13 seconds
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Generative Artificial Intelligence: More of the Same or Off the Control Chart?

10/5/202317 minutes, 59 seconds
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Short Timeframe Prediction of Kidney Failure among Patients with Advanced Chronic Kidney Disease

9/29/202310 minutes, 45 seconds
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Audio Summary October 2023

9/19/20235 minutes, 22 seconds
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Reference Interval Harmonization: Harnessing the Power of Big Data Analytics to Derive Common Reference Intervals across Populations and Testing Platforms

9/18/202310 minutes, 42 seconds
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A Machine Learning Model for the Routine Detection of “Wrong Blood in Complete Blood Count Tube” Errors

9/8/202312 minutes, 34 seconds
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In-Hospital Manufacturing of Cellular Therapies Using Automated Systems

9/5/202311 minutes, 11 seconds
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Audio Summary September 2023

8/21/20237 minutes, 24 seconds
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Feasibility of Targeted Next-Generation DNA Sequencing for Expanding Population Newborn Screening

8/16/20236 minutes, 15 seconds
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Abatacept as a Mimic of Gamma Heavy Chain Disease

8/7/20239 minutes, 27 seconds
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Assessing the Accuracy and Clinical Utility of ChatGPT in Laboratory Medicine

8/1/202313 minutes, 15 seconds
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Audio Summary August 2023

7/17/20235 minutes, 12 seconds
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Machine Learning in Laboratory Medicine: Recommendations of the IFCC Working Group

7/13/20236 minutes, 42 seconds
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Driving Under the Influence of Cannabis: Impact of Combining Toxicology Testing with Field Sobriety Tests

7/3/202312 minutes, 9 seconds
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Tissue Mass Spectrometry: How Solid Is Our Future?

6/28/202316 minutes, 1 second
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Audio Summary July 2023

6/14/20237 minutes, 46 seconds
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Polygenic Risk Scores: Genomes to Risk Prediction

6/13/202313 minutes, 9 seconds
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Rapid Rule-Out of Myocardial Infarction Using a Single High-Sensitivity Cardiac Troponin I Measurement Strategy at Presentation to the Emergency Department: The SAFETY Study

6/5/202312 minutes, 38 seconds
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Using Biomarkers for Traumatic Brain Injury: Are There Lessons That Can Be Learned from Implementing Cardiac Troponin?

5/31/20236 minutes, 32 seconds
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Audio Summary June 2023

5/15/20236 minutes, 53 seconds
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The Present and Future of Lipid Testing in Cardiovascular Risk Assessment

5/5/202312 minutes, 18 seconds
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Multiplexing Homocysteine into First-Tier Newborn Screening Mass Spectrometry Assays Using Selective Thiol Derivatization

5/5/20238 minutes, 17 seconds
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Lab Medicine in Space

4/26/202312 minutes, 1 second
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Audio Summary May 2023

4/12/20237 minutes, 49 seconds
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FDA Regulation of Laboratory Clinical Decision Support Software: Is It a Medical Device?

4/10/202314 minutes, 20 seconds
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The Genotype—Phenotype Conundrum for Antimicrobial Susceptibility Testing

3/31/202310 minutes, 1 second
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Psychedelics for Medicinal Use: How Will This Alter the Collective Laboratory Consciousness?

3/28/20239 minutes, 40 seconds
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Audio Summary April 2023

3/15/20237 minutes, 16 seconds
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Clinical Perspective on Use of Long-Read Sequencing in Prenatal Diagnosis of Thalassemia

3/9/20237 minutes, 57 seconds
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Effects of Supplemented Mediterranean Diets on Plasma-Phospholipid Fatty Acid Profiles and Risk of Cardiovascular Disease after 1 Year of Intervention in the PREDIMED Trial

2/28/20239 minutes, 4 seconds
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A Spotlight on Disruptors and Innovators

2/28/20239 minutes, 35 seconds
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Audio Summary March 2023

2/13/20236 minutes, 11 seconds
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Fragment Ends of Circulating Microbial DNA as Signatures for Pathogen Detection in Sepsis

2/2/202314 minutes, 54 seconds
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Laboratory Preparedness for the Current Monkeypox Outbreak

2/1/202313 minutes, 30 seconds
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Within-Person and Between-Sensor Variability in Continuous Glucose Monitoring Metrics

1/30/20234 minutes, 55 seconds
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A Translational Tool to Facilitate Use of Apolipoprotein B for Clinical Decision-Making

1/24/20239 minutes, 32 seconds
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Audio Summary February 2023

1/19/20237 minutes, 24 seconds
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Neutral-Coating Capillary Electrophoresis Coupled with High-Resolution Mass Spectrometry for Top-Down Identification of Hemoglobin Variants

1/13/202311 minutes, 52 seconds
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One Bad Apple Can Spoil the Bunch: The Impact of Contamination in the Clinical Laboratory

1/11/202313 minutes
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Can Rapid Nanopore Sequencing Bring Genomic Testing to the Bedside?

1/3/202311 minutes, 44 seconds
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What is the Importance of Analyzing Tumor Tissues and Blood Cells in the Study of Circulating Tumor-Derived DNA?

1/3/20239 minutes, 23 seconds
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Audio Summary January 2023

12/19/20226 minutes, 41 seconds
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Rising to the Challenge: Shortages in Laboratory Medicine

12/5/202221 minutes, 36 seconds
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Ceramide Risk Scores Can Bring Lipidomics to Clinical Medicine

11/30/202212 minutes, 20 seconds
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Audio Summary December 2022

11/18/20227 minutes, 10 seconds
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Identifying Clinically Relevant Bacteria Directly from Culture and Clinical Samples with a Handheld Mass Spectrometry Probe

11/11/202221 minutes, 11 seconds
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Macrotroponin—Analytical Anomaly or Clinical Confounder

11/8/20228 minutes, 39 seconds
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The PROVE-HF Study: Additional Proof for the Inclusion of the Manufacturer’s Name When Reporting B-Type Natriuretic Peptide Results

10/27/202211 minutes, 50 seconds
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Clinical Laboratory Informatics and Analytics: Challenges and Opportunities

10/26/202210 minutes, 47 seconds
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Audio Summary November 2022

10/19/20227 minutes, 9 seconds
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Cardiac Troponin as a Marker of Heart Failure Risk in Diabetes

10/17/20228 minutes, 53 seconds
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Estimated Atherosclerotic Cardiovascular Disease Risk Score: An Automated Decision Aid for Statin Therapy

10/3/202211 minutes, 22 seconds
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Harmonization Status of Serum Ferritin Measurements and Implications for Use as Marker of Iron-Related Disorders

10/3/202212 minutes, 5 seconds
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Audio Summary October 2022

9/22/20227 minutes, 33 seconds
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Differences between Educational and Regulatory External Quality Assurance/Proficiency Testing Schemes

9/20/202214 minutes, 26 seconds
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Pan-Cancer Detection and Typing by Mining Patterns in Large Genome-Wide Cell-Free DNA Sequencing Datasets

9/16/202211 minutes, 44 seconds
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Rh Blood Group D Antigen Genotyping Using a Portable Nanopore-based Sequencing Device: Proof of Principle

9/8/202212 minutes, 24 seconds
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Direct-to-Consumer Testing for Routine Purposes

9/6/202213 minutes, 57 seconds
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Multiplex Fragment Analysis for Flexible Detection of All SARS-CoV-2 Variants of Concern

8/30/20229 minutes, 29 seconds
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Audio Summary September 2022

8/24/20227 minutes, 43 seconds
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Reproducibility: Has Cancer Biology Failed beyond Repair?

8/22/202210 minutes, 53 seconds
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Public Misinformation and Science Communication in Times of Public Health Crises

8/22/202210 minutes, 59 seconds
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Women in Laboratory Medicine: A Q&A on Diversity and Inclusion

8/18/202215 minutes, 56 seconds
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Jagged Ends on Multinucleosomal Cell-Free DNA Serve as a Biomarker for Nuclease Activity and Systemic Lupus Erythematosus

8/4/202210 minutes, 25 seconds
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Critical Roles of Clinical Laboratorians in Translational Research and Applied Sciences—Experts’ Insights and Experiences

8/1/202215 minutes, 24 seconds
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Machine Learning to Assist in Large-Scale, Activity-Based Synthetic Cannabinoid Receptor Agonist Screening of Serum Samples

7/29/202212 minutes, 33 seconds
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The Need to Develop Clinical Guidance for the Use of High-Sensitivity Cardiac Troponin in Pediatric and Neonatal Patients

7/29/202213 minutes, 38 seconds
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Audio Summary August 2022

7/11/20228 minutes, 23 seconds
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Audio Summary July 2022

6/13/20227 minutes, 40 seconds
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Evaluation of Neutralizing Antibodies against SARS-CoV-2 Variants after Infection and Vaccination Using a Multiplexed Surrogate Virus Neutralization Test

6/3/202214 minutes, 53 seconds
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Angiogenic Biomarkers for Risk Stratification in Women with Preeclampsia

5/31/20229 minutes, 29 seconds
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A Novel Approach to Improve Accuracy of CYP2D6 Enzyme Activity and Drug Response Predictions

5/20/202217 minutes, 42 seconds
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Audio Summary June 2022

5/18/20227 minutes, 14 seconds
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Toward Clinical Application of Leukocyte Counts Based on Targeted DNA Methylation Analysis

5/12/202211 minutes, 46 seconds
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How Not to Introduce Laboratory Tests to Clinical Practice: Preimplantation Genetic Testing for Aneuploidy

5/3/202214 minutes, 43 seconds
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New Equations for Estimating Glomerular Filtration Rate

5/2/202216 minutes, 46 seconds
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A Paradigm Shift: Engagement of Clinical Chemistry and Laboratory Medicine Trainees by Innovative Teaching Methods

4/25/20229 minutes, 59 seconds
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Glycated Albumin: Added Value or Redundancy in Diabetes Care?

4/25/202212 minutes, 16 seconds
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Interpreting EQA—Understanding Why Commutability of Materials Matters

4/21/202215 minutes, 10 seconds
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How Can We Ensure Reproducibility and Clinical Translation of Machine Learning Applications in Laboratory Medicine?

4/13/202210 minutes, 15 seconds
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Audio Summary May 2022

4/13/20227 minutes, 32 seconds
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Pregnancy in the Time of COVID-19: What Are the Challenges for Maternity Care?

3/28/202215 minutes, 40 seconds
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Audio Summary April 2022

3/21/20228 minutes, 8 seconds
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Alternative Sample Matrices Supporting Remote Sample Collection during the Pandemic and Beyond

3/7/202210 minutes, 33 seconds
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Galaxy Is a Suitable Bioinformatics Platform for the Molecular Diagnosis of Human Genetic Disorders Using High-Throughput Sequencing Data Analysis: Five Years of Experience in a Clinical Laboratory

2/24/202210 minutes, 4 seconds
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Audio Summary March 2022

2/15/20226 minutes, 24 seconds
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Chest Pain Assessment: What Is Our Endgame?

2/8/202215 minutes, 41 seconds
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Deadly Pathogens, Transformative Technologies, and Protracted Pandemics: Challenges and Opportunities in Laboratory Medicine

1/25/202211 minutes, 45 seconds
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At-Home Testing for Infectious Diseases: The Laboratory Where You Live

1/19/202222 minutes, 50 seconds
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Audio Summary February 2022

1/18/20229 minutes, 8 seconds
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A Push for Real Normal: Mass Screening for COVID-19

1/18/202211 minutes, 23 seconds
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Audio Summary January 2022

12/21/202111 minutes, 24 seconds
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Challenges in Translating Clinical Metabolomics Data Sets from the Bench to the Bedside

12/13/202113 minutes, 31 seconds
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Digital Microfluidic Hemagglutination Assays for Blood Typing, Donor Compatibility Testing, and Hematocrit Analysis

12/7/202114 minutes, 4 seconds
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Circulating Ceramides and Sphingomyelins and Risk of Mortality: The Cardiovascular Health Study

12/6/202116 minutes, 33 seconds
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Molecular Approaches to Transplant Monitoring; Is the Horizon Here?

12/6/202110 minutes, 32 seconds
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Audio Summary December 2021

11/12/202111 minutes, 4 seconds
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Liquid Biopsy Hotspot Variant Assays: Analytical Validation for Application in Residual Disease Detection and Treatment Monitoring

11/12/202111 minutes, 36 seconds
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Programmed Cell Death Ligand 1-Expressing Circulating Tumor Cells: A New Prognostic Biomarker in Non-Small Cell Lung Cancer

11/11/20218 minutes, 55 seconds
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The European Biological Variation Study (EuBIVAS): Biological Variation Data for Coagulation Markers Estimated by a Bayesian Model

11/9/20219 minutes, 34 seconds
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Perspectives on Identifying and Treating Familial Hypercholesterolemia in Childhood

11/1/202110 minutes, 26 seconds
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Audio Summary November 2021

10/11/20218 minutes, 54 seconds
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Can High-Sensitivity Troponins Help to Level the Playing Field in Cardiovascular Disease Prevention between Women and Men?

9/28/20219 minutes, 20 seconds
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TOP-Plus is a Versatile Biosensor Platform for Monitoring SARS-CoV-2 Antibody Durability

9/17/202114 minutes, 45 seconds
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Audio Summary October 2021

9/7/20218 minutes, 36 seconds
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High-Sensitivity Cardiac Troponin T for the Detection of Myocardial Injury and Risk Stratification in COVID-19

8/27/202116 minutes, 39 seconds
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Cardiac Biomarkers in Pediatrics: An Undervalued Resource

8/24/202110 minutes
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Audio Summary September 2021

8/17/20219 minutes, 20 seconds
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AACC Practical Recommendations for Implementing and Interpreting SARS-CoV-2 EUA and LDT Serologic Testing in Clinical Laboratories

8/16/202114 minutes, 54 seconds
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How Well Do Laboratories Adhere to Recommended Guidelines for Cardiac Biomarkers Management in Europe? The CArdiac MARker Guideline Uptake in Europe (CAMARGUE) Study of the European Federation of Laboratory Medicine Task Group on Cardiac Markers

8/13/202110 minutes, 22 seconds
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Big Data Strikes Again: Future Utilization of the UK Biobank as a Resource for Clinical Laboratories

8/6/20215 minutes, 46 seconds
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Case-Control Study of Individuals with Discrepant Nucleocapsid and Spike Protein SARS-CoV-2 IgG Results

8/5/20218 minutes, 40 seconds
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Serum Prostate-Specific Antigen Testing for Early Detection of Prostate Cancer: Managing the Gap Between Clinical and Laboratory Practice

7/16/202111 minutes, 49 seconds
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Audio Summary August 2021

7/16/20219 minutes, 57 seconds
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Associating SARS-CoV-2 Serological Assays with Protection: Where the Field Stands

7/2/202113 minutes, 1 second
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Laboratory Action Plan for Emerging SARS-CoV-2 Variants

6/28/202113 minutes, 9 seconds
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Audio Summary July 2021

6/24/20218 minutes, 17 seconds
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Centralized vs Decentralized Molecular Infectious Disease Testing

6/15/202110 minutes, 21 seconds
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Is it Time to move on? Reexamining Race in Glomerular Filtration Rate Equations

6/14/202114 minutes, 20 seconds
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Limitations of Accelerated Stability Model Based on the Arrhenius Equation for Shelf Life Estimation of In Vitro Diagnostic Products

6/1/202112 minutes, 12 seconds
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Interoperability: COVID-19 as an Impetus for Change.

6/1/202111 minutes, 4 seconds
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Audio Summary June 2021

5/17/20218 minutes, 49 seconds
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Audio Summary May 2021

4/21/20218 minutes, 25 seconds
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Development of an LC-MS/MS Proposed Candidate Reference Method for the Standardization of Analytical Methods to Measure Lipoprotein(a)

4/20/202118 minutes, 58 seconds
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Clinical Protein Analysis by Mass Spectrometry: A New Higher Order

4/12/202122 minutes, 40 seconds
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Audio Summary April 2021

3/29/20219 minutes, 32 seconds
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Towards an SI-Traceable Reference Measurement System for Seven Serum Apolipoproteins Using Bottom-Up Quantitative Proteomics: Conceptual Approach Enabled by Cross-Disciplinary/Cross-Sector Collaboration

3/28/202116 minutes, 38 seconds
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Personalized Reference Intervals in Laboratory Medicine: A New Model Based on Within-Subject Biological Variation

2/26/202110 minutes, 6 seconds
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The Dynamic and Multifaceted Nature of Cardiovascular Disease and Using Genetic Testing to Inform Clinical Care: An International Perspective

2/26/202116 minutes, 21 seconds
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Audio Summary March 2021

2/26/20218 minutes, 12 seconds
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The Vitamin D Metabolite Ratio is Independent of Vitamin D Binding Protein Concentration

1/31/202112 minutes, 5 seconds
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Audio Summary February 2021

1/26/20218 minutes, 8 seconds
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Nonfasting Lipid Profile for all Patients?

1/2/202110 minutes, 15 seconds
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B-Type Natriuretic Peptide Is Associated with Favorable Left Ventricular Function in General Population

1/2/202110 minutes, 58 seconds
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Audio Summary January 2021

12/23/202013 minutes, 12 seconds
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Deus ex machina? Predicting SARS-CoV-2 Infection from Lab Tests Using Machine Learning.

12/8/20206 minutes, 53 seconds
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SARS-CoV-2 Whole Genome Amplification and Sequencing for Effective Population-Based Surveillance and Control of Viral Transmission

12/8/202013 minutes, 56 seconds
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Cautionary Note on Contamination of Reagents Used for Molecular Detection of SARS-CoV-2

12/8/202015 minutes, 39 seconds
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Genomic Prediction of Antimicrobial Resistance: Ready or Not, Here It Comes!

12/8/202013 minutes, 59 seconds
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The Faces of Women in AACC - A Historical Reflection on AACC Leadership and Awards

12/3/202016 minutes, 12 seconds
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Audio Summary December 2020

11/25/20208 minutes, 45 seconds
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Cell-free DNA fragmentomics: the new “omics” on the block

11/13/20209 minutes, 27 seconds
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Audio Summary November 2020

10/26/20208 minutes, 38 seconds
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Examining Laboratory Medicine's Role in Eliminating Health Disparities

10/16/202010 minutes, 21 seconds
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Proportion of Donor Specific Cell-Free DNA in Blood as a Marker of Transplant Rejection: Not an Absolute

10/7/20207 minutes, 39 seconds
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Evaluation of the Risk of Laboratory Microbial Contamination During Routine Testing in Automated Clinical Chemistry and Microbiology Laboratories

9/30/202010 minutes, 31 seconds
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Patient-Based Real Time QC

9/30/202024 minutes, 26 seconds
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Audio Summary October 2020

Listen on the go: Audio Summary of the original articles from the October 2020 issue. 
9/29/20207 minutes, 26 seconds
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In-Depth Analysis of Molecular Heterogeneity of Circulating N-Terminal pro-BNP: Does Detailed Characterization of Analyte Structure Really Matter for its Diagnostic Use?

9/4/20209 minutes, 7 seconds
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Throughput Considerations for a Sample-Multiplexed LC-MS/MS Assay: Is the Ability to Double the Injection Throughput Always a Time Saver?

9/4/20209 minutes, 12 seconds
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Recognition of the Pre-hospital Preanalytical Phase: Collaborative Efforts between Laboratory Medicine and Emergency Medicine to Ensure Quality Testing

8/31/202017 minutes, 33 seconds
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Clinical Performance of Two SARS-CoV-2 Serologic Assays

8/20/202015 minutes, 37 seconds
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Audio Summary September 2020

8/20/20207 minutes, 5 seconds
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Machine Learning for the Biochemical Genetics Laboratory

8/17/20208 minutes, 52 seconds
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Audio Summary August 2020

7/27/20207 minutes, 51 seconds
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IFCC Working Group Recommendations for Correction of Bias Caused by Noncommutability of a Certified Reference Material Used in the Calibration Hierarchy of an End-User Measurement Procedure

7/9/20208 minutes, 28 seconds
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Fecal Microbiota Transplantations: Where Are We, Where Are We Going, and What Is the Role of the Clinical Laboratory?

7/9/202010 minutes, 23 seconds
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Modification of In Vitro Diagnostic Devices: Leveling the Playing Field

7/9/20209 minutes, 30 seconds
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Audio Summary July 2020

6/25/20206 minutes, 54 seconds
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July 2020 Summary Peddu

6/25/20201 minute, 1 second
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July 2020 Summary Caswell

6/25/202056 seconds
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July 2020 Summary Pitella-Silva

6/25/202050 seconds
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July 2020 Summary Lau

6/25/202055 seconds
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July 2020 Summary Jensen

6/25/202055 seconds
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July 2020 Summary McGrath

6/25/202056 seconds
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June 2020 Summary Benoist

5/26/202059 seconds
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June 2020 Summary Min

5/26/20201 minute, 4 seconds
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June 2020 Summary Crawford

5/26/202050 seconds
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June 2020 Summary Tafess

5/26/20201 minute, 10 seconds
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June 2020 Summary Trouillet-Assant

5/26/202034 seconds
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June 2020 Summary Pan

5/26/20201 minute
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Audio Summary June 2020

5/26/20206 minutes, 44 seconds
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SARS-CoV-2 Serology: Much Hype, Little Data

5/14/202012 minutes, 52 seconds
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Development, Validation, and Regulatory Considerations for a Liquid Biopsy Test

5/12/20208 minutes, 35 seconds
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Audio Summary May 2020

4/24/20207 minutes, 38 seconds
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May 2020 Summary Clouet-Foraison

4/24/20201 minute, 16 seconds
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May 2020 Summary Liu

4/24/202057 seconds
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May 2020 Summary Guo

4/24/20201 minute, 10 seconds
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May 2020 Summary Heider

4/24/202055 seconds
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May 2020 Summary Hicks

4/24/20201 minute, 1 second
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May 2020 Summary Colak

4/24/202047 seconds
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Calibrating from Within: Multipoint Internal Calibration of a Quantitative Mass Spectrometric Assay of Serum Methotrexate

3/31/20207 minutes, 2 seconds
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Audio Summary April 2020

3/26/20208 minutes, 3 seconds
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April 2020 Summary Silveira

3/26/202056 seconds
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April 2020 Summary Lam

3/26/20201 minute, 7 seconds
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April 2020 Summary Korecka

3/26/20201 minute, 7 seconds
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April 2020 Summary Moncrieffe

3/26/20201 minute, 2 seconds
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April 2020 Summary Lyngbakken

3/26/20201 minute, 1 second
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April 2020 Summary Jongejan

3/26/202049 seconds
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April 2020 Summary Chu

3/26/202047 seconds
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Mass Spectrometry for Identification, Monitoring, and Minimal Residual Disease Detection of M-Proteins

3/17/20209 minutes, 10 seconds
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Clinical Relevance of the 99th Percentile Upper Reference Limit for High-Sensitivity Cardiac Troponin Assays

3/13/20208 minutes, 21 seconds
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Emergence of a Novel Coronavirus Disease (COVID-19) and the Importance of Diagnostic Testing: Why Partnership between Clinical Laboratories, Public Health Agencies, and Industry Is Essential to Control the Outbreak [Chinese Translation]

3/10/202011 minutes, 45 seconds
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Sex-Specific 99th Percentile Upper Reference Limits for High Sensitivity Cardiac Troponin Assays Derived Using a Universal Sample Bank

3/10/202019 minutes, 18 seconds
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Development and Clinical Validation of a Sensitive Lateral Flow Assay for Rapid Urine Fentanyl Screening in the Emergency Department

2/28/20208 minutes, 39 seconds
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Emergence of a Novel Coronavirus Disease (COVID-19) and the Importance of Diagnostic Testing: Why Partnership between Clinical Laboratories, Public Health Agencies, and Industry Is Essential to Control the Outbreak

2/27/202011 minutes, 47 seconds
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Audio Summary March 2020

2/26/20207 minutes, 37 seconds
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March 2020 Summary Guo

2/26/20201 minute, 7 seconds
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March 2020 Summary Hoffman

2/26/202058 seconds
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March 2020 Summary Blesa

2/26/202051 seconds
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March 2020 Summary Sun

2/26/20201 minute, 6 seconds
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March 2020 Summary Lam

2/26/20201 minute, 2 seconds
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March 2020 Summary Apple

2/26/20201 minute, 15 seconds
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Molecular Diagnosis of a Novel Coronavirus (2019-nCoV) Causing an Outbreak of Pneumonia [Chinese Translation]

Chinese Translation of "Molecular Diagnosis of a Novel Coronavirus (2019-nCoV) Causing an Outbreak of Pneumonia" interview with Dr. Leo Poon.
1/31/20208 minutes, 4 seconds
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Molecular Diagnosis of a Novel Coronavirus (2019-nCoV) Causing an Outbreak of Pneumonia

In December 2019, a cluster of atypical pneumonia patients epidemiologically linked to a wholesale market in Wuhan, China was detected. A novel betacoronavirus named as 2019-novel coronavirus (2019-nCoV) has been identified in some of these patients. Considerable attention has been given to this virus, that according to the World Health Organization as of this recording at the end of January 2020, has so far thought to have sickened over 6,000 people and is responsible for at least 132 deaths. Because of the potential for pandemic spread, there is a great need for a rapid and accurate test for the detection of the virus. We are fortunate to have with us today Dr. Leo Poon from the School of Public Health, Li Ka Shing Faculty of Medicine at the University of Hong Kong. He and his colleagues described a new molecular diagnostic assay that allows the detection and quantification of this new coronavirus.
1/31/20207 minutes, 42 seconds
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February 2020 Summary Pruner

1/29/20201 minute, 10 seconds
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February 2020 Summary Jin

1/29/202056 seconds
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February 2020 Summary Malka

1/29/202053 seconds
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February 2020 Summary Vigneron

1/29/202056 seconds
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February 2020 Summary Ge

1/29/20201 minute, 13 seconds
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February 2020 Summary Starnberg

1/29/20201 minute, 5 seconds
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February 2020 Summary Li

1/29/20201 minute, 1 second
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February 2020 Summary O'Malley

1/29/202055 seconds
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Audio Summary February 2020

1/29/20209 minutes, 23 seconds
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Circulating Tumor DNA for Modern Cancer Management

Molecular diagnostics is the topic of the January 2020 special issue of Clinical Chemistry.  In that issue, there is a counter point report on the use of circulating tumor cells in the management of cancer patients.  To provide further insight on this topic, we’re joined in this podcast by one of the editors of this special issue, Professor Klaus Pantel, Chairman of the Institute of Tumor Biology at the University Medical Center in Hamburg-Eppendorf in Germany. 
1/3/20206 minutes, 53 seconds
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Microbiome Diagnostics

Breakthroughs in sequencing technology and computational biology have provided the basis for studies of the myriad ways in which microbial communities in and on the human body influence human health and disease. In almost every medical specialty, there is now growing interest in accurate and replicable profiling of the microbiome for use in diagnostic and therapeutic application.           In the January 2020 issue of Clinical Chemistry, which is devoted to topics in molecular diagnostics, Dr. Robert Schlaberg reviews microbiome diagnostics.
1/3/202010 minutes, 46 seconds
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Unconventional Diagnosis Based on Somatic Findings through Germ Line Whole-Exome Sequencing

Molecular diagnostics is now firmly entrenched in laboratory medicine and the January 2020 issue of Clinical Chemistry is devoted to this topic.  In that issue is a case report that presents an unconventional diagnosis based somatic findings through germline whole-exome sequencing.  Joining us in this podcast is the lead author of that case report, Dr. Jaime Lopes, who is currently a fellow at the Genomics Laboratory in the Department of Laboratory Medicine and Pathology at the Mayo Clinic in Rochester, Minnesota. 
1/3/202010 minutes, 49 seconds
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Predictive and Precision Medicine with Genomic Data

Molecular diagnostics is now firmly rooted in laboratory medicine and the January 2020 issue of Clinical Chemistry is devoted to this topic.  In that issue, Dr. Linnea Baudhuin reviews genome and exome sequencing as they’re used in clinical practice.
1/3/202020 minutes, 12 seconds
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Molecular Diagnostics: Going from Strength to Strength

Molecular Diagnostics is clearly going from strength to strength.  That’s the title of the lead article by the guest editors in the January 2020 issue of Clinical Chemistry that is devoted to this topic.  It has been five years since the last molecular diagnostics theme issue was published in Clinical Chemistry.  In this new edition of the theme issue, the editors have highlighted some of the most exciting developments in the field including the maturation of massively parallel DNA sequencing technology allowing production of genomic data at the hospital or even population level. Joining us in this podcast is one of the guest editors for this special issue, Dr. Carl Wittwer. 
1/3/202013 minutes, 22 seconds
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Audio Summary January 2020

12/31/201912 minutes, 16 seconds
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January 2020 Summary Coelho-Lima

12/31/20191 minute, 11 seconds
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January 2020 Summary Wu

12/31/20191 minute, 4 seconds
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January 2020 Summary Profaizer

12/31/20191 minute
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January 2020 Summary Zucha

12/31/20191 minute, 8 seconds
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January 2020 Summary Chandler

12/31/201953 seconds
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January 2020 Summary Gotway

12/31/201949 seconds
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January 2020 Summary Ge

12/31/201940 seconds
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January 2020 Summary Song

12/31/201957 seconds
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January 2020 Summary Lin

12/31/20191 minute, 15 seconds
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January 2020 Summary Joosse

12/31/201954 seconds
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January 2020 Summary Lampignano

12/31/20191 minute, 3 seconds
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Ethics for Laboratory Medicine

Maintaining high ethical standards is a characteristic we expect in many professionals, but perhaps most in the field of medicine.  Although biomedical ethics is a relatively new field, there have been discussions of moral issues in medicine since ancient times.  The Hippocratic Oath, for instance, was written by Hippocrates at approximately 400 B.C.  It requires that a new physician swear to uphold specific ethical standards.  Over 2000 years later new physicians still swear by the Hippocratic or a similar oath. The December 2019 issue of Clinical Chemistry published a Review paper that provides a broad overview of ethics as it pertains to laboratory medicine.
12/3/201910 minutes, 21 seconds
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Laboratory Measurement's Contribution to the Replication and Application Crisis in Clinical Research

Science is not just based on interesting findings.  One of the key elements in research reports is an informative description of the methods that were used to develop the experiment, to collect the data, and to analyze them.  Since this process rests on replication, reporting of methods is essential as it allows others to stage a similar experiment, to design an identical study, and to evaluate whether the findings can be replicated.  If they cannot be reproduced, the outcomes from the previous experiment or trial will gradually or immediately lose credibility.   A recent editorial on this topic appeared in the December 2019 issue of Clinical Chemistry. 
12/2/20197 minutes, 46 seconds
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Audio Summary December 2019

11/19/201910 minutes, 28 seconds
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December 2019 Summary Sorensen

11/19/20191 minute, 1 second
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December 2019 Summary Meisterzheim

11/19/201954 seconds
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December 2019 Summary Weber

11/19/201948 seconds
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December 2019 Summary Salamin

11/19/201956 seconds
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December 2019 Summary Sun

11/19/201956 seconds
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December 2019 Summary Yang

11/19/20191 minute, 30 seconds
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December 2019 Summary Wussler

11/19/201948 seconds
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December 2019 Summary Hughey

11/19/201959 seconds
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December 2019 Summary Costenbader

11/19/20191 minute, 8 seconds
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Circular RNAs as Urinary Biomarkers

Circular RNAs have the unique topological feature of circularity among the RNAs species.  They are single-stranded, covalently closed, circular RNA molecules and are hypothesized to be generated largely through back-splicing of exons from precursor messenger RNAs.  Circular RNAs were once considered as aberrant splicing by-products, which were only present in minute amounts in cells.  The potential of this RNAs species as a disease biomarker is to be explored, but there are biological properties of circular RNAs which make them suitable for biomarker development. An Editorial appearing in the October 2019 issue of Clinical Chemistry by Drs. Jacky Lam and Dennis Lo discussed circular RNAs as potential urinary biomarkers. 
11/6/201913 minutes, 11 seconds
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ProBNP That Is Not Glycosylated at Threonine 71 Is Decreased with Obesity in Patients with Heart Failure

Heart failure is a leading cause of morbidity and mortality worldwide with prevalence expected to increase over the next 20 years.  Early diagnosis and optimal management of heart failure are key to reducing its impact. Because B-type natriuretic peptide, or BNP, and amino terminal proBNP, or NT-proBNP, as well as their precursor proBNP, are secreted by the heart in direct proportion to the degree of cardiac dysfunction and clinical severity, measurement of these peptides is now mandated by authoritative international guidelines for the diagnosis and risk stratification of the disease.  However, circulating concentrations of both BNP and NT-proBNP are reduced by obesity, and this phenomenon is one of the key weaknesses of the diagnostic performance of the natriuretic peptides in heart failure. A paper appearing in the September 2019 issue of Clinical Chemistry shows that obesity is associated with decreased concentrations of proBNP that is not glycosylated at threonine at position 71 of the peptide.  Decreased proBNP substrate amenable to processing could partially explain the lower NT-proBNP and BNP concentrations measured in obese individuals, including those presenting with heart failure.  
11/6/201914 minutes, 6 seconds
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An Intact ACTH LC-MS/MS Assay as an Arbiter of Clinically Discordant Immunoassay Results

Measurement of plasma adrenocorticotropic hormone, or ACTH, is key in the differential diagnosis of hypothalamic pituitary adrenal disorders. Two-site sandwich immunoassay dominate clinical testing of ACTH in North America.  However, discordant results among different assays have been repeatedly reported.  To help resolve this discrepancy, a multicenter effort developed a liquid chromatography tandem mass spectrometry assay for the intended measurand, that is the biologically active intact ACTH.  A report on that initiative appears in the November 2019 issue of Clinical Chemistry.  We are pleased to have the senior author of that paper , Dr. Mari DeMarcoas our guest in this podcast. 
11/1/20196 minutes, 16 seconds
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Audio Summary November 2019

10/22/20199 minutes, 32 seconds
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November 2019 Summary Bansal

10/22/20191 minute
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November 2019 Summary Nestelberger

10/22/20191 minute, 11 seconds
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November 2019 Summary Boeddinghaus

10/22/20191 minute, 13 seconds
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November 2019 Summary Yang

10/22/20191 minute, 7 seconds
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November 2019 Summary O' Leary

10/22/201956 seconds
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November 2019 Summary Shi

10/22/201949 seconds
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November 2019 Summary van Faassen

10/22/201957 seconds
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November 2019 Summary Potter

10/22/201957 seconds
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A View on the Interrelationship between Obesity and Natriuretic Peptide Measurements: Can Dysregulation in pro–B-type Natriuretic Peptide Glycosylation Explain Decreased B-type Natriuretic Peptide Concentrations in Obese Heart Failure Patients?

Obesity is on the rise worldwide and it’s a risk factor for systemic hypertension, hyperlipidemia, diabetes mellitus, and left ventricular hypertrophy, all of which are conditions associated with an increased prevalence of heart failure.  However, the applications of BNP and NT-proBNP as biomarkers in obese patients are limited, as the relationship between their levels and myocardial stiffness is complex.  An Editorial appearing in the September 2019 issue of Clinical Chemistry examines the interrelationship between obesity and BNP and NT-proBNP measurements.  
10/11/20199 minutes, 7 seconds
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Urinary Amino-Terminal Pro–C-Type Natriuretic Peptide: A Novel Marker of Chronic Kidney Disease in Diabetes

A paper appearing in the October 2019 issue of Clinical Chemistry found that fragments of proCNP could be measured in urine from subjects with diabetes mellitus.  The urinary NT-proCNP to creatinine ratio was also more reproduceable than the now commonly used albumin to creatinine ratio and strongly associated with the presence of chronic kidney disease. 
10/11/20198 minutes, 20 seconds
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Role of BNP vs NT-proBNP Testing in the Age of New Drug Therapies: Sacubitril-Valsartan

Sacubitril/Valsartan, known as Entresto, is a new dual drug therapy that includes an angiotensin receptor inhibitor and is indicated to reduce the risk of cardiovascular death and hospitalization in patients with chronic heart failure.  Since its approval for the treatment of chronic heart failure with reduced injection fraction, a commonly raised question is whether treatment with this drug challenges the use of B-type natriuretic peptide, or BNP, testing compared to the N terminal proBNP assay because Sacubitril may interfere with BNP clearance. The clinical and analytical studies addressing this issue are limited, along with the fact the diversity of both BNP and NT-proBNP assays used in clinical laboratory practice have not been adequately evaluated in clinical trials or studies to provide an evidenced-based on final decision as to what assay or assays should be used or eliminated from use when a patient is on Entresto. In the September 2019 issue of Clinical Chemistry, a Q&A feature titled, “Role of BNP vs NT-proBNP Testing in the Age of New Drug Therapies” asked five experts with different roles in this field to discuss this issue. 
10/10/20198 minutes, 23 seconds
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Once-Per-Visit Alerts: A Means to Study Alert Compliance and Reduce Repeat Laboratory Testing

Overutilization of laboratory services is an important unresolved issue in health care.  A recent study that appears in the September 2019 issue of Clinical Chemistry attempts to address this issue.  That paper titled ” Once-Per-Visit Alerts: A Means to Study Alert Compliance and Reduce Repeat Laboratory Testing,” examined reorders of a laboratory test within the same admission. 
10/10/20198 minutes, 19 seconds
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Detection of Neprilysin-Derived BNP Fragments in the Circulation: Possible Insights for Targeted Neprilysin Inhibition Therapy for Heart Failure

B-type natriuretic peptides, or BNP, and N-terminal proBNP, or NT-proBNP, are peptides produced in the heart in response to increased wall stretch and volume overload.  Their production and secretion increases in the heart with the progression of heart failure and they have emerged as useful heart failure biomarkers.  Since the discovery of BNPs in the 1980s, much effort has been made to precisely determine the BNP and NT-proBNP levels via immunoassays for reliable heart failure diagnostics. Entresto™ is a new heart failure therapy that includes sacubitril as one of its components.  Sacubitril is a specific inhibitor of neprilysin. This is a zinc-dependent metalloproteinase that cleaves various peptides including BNP.  In fact, augmentation of circulating BNP due to neprilysin inhibition is considered as a possible mechanism of Entresto’s positive effects.  A paper appearing in the October 2019 issue of Clinical Chemistry examines the circulating products of BNP proteolysis by neprilysin and how they might reflect impact on the metabolism of active BNP. 
10/1/20199 minutes, 58 seconds
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Audio Summary October 2019

9/20/201911 minutes, 3 seconds
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October 2019 Summary Hoq

9/20/201949 seconds
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October 2019 Summary Takahashi

9/20/20191 minute, 5 seconds
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October 2019 Summary van Tol

9/20/20191 minute, 11 seconds
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October 2019 Summary Kolling

9/20/20191 minute, 9 seconds
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October 2019 Summary Sun

9/20/20191 minute, 13 seconds
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October 2019 Summary Garrel

9/20/20191 minute, 2 seconds
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October 2019 Summary Chan

9/20/20191 minute, 13 seconds
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October 2019 Summary Prickett

9/20/20191 minute, 3 seconds
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October 2019 Summary Feygina

9/20/20191 minute, 9 seconds
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Optimal Use of Biomarkers for Chronic Kidney Disease

In the August 2019 issue of Clinical Chemistry, a Q&A feature titled “Optimal Use of Biomarker for Chronic Kidney Disease” asked five experts in laboratory medicine and nephrology to examine laboratory ordering, testing, and reporting practices, and recommend how those practices should be optimized to better serve patients with CKD. In this podcast, Dr. Greg Miller from the Department of Pathology at Virginia Commonwealth University in Richmond who moderated the Q&A will summarize the expert advice from his colleagues. 
8/23/201916 minutes, 51 seconds
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September 2019 Summary Lynch

8/21/201956 seconds
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September 2019 Summary Ma

8/21/201944 seconds
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September 2019 Summary Cassinari

8/21/201952 seconds
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September 2019 Summary Lampasona

8/21/20191 minute, 1 second
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September 2019 Summary Lampignano

8/21/20191 minute, 7 seconds
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September 2019 Summary Szymanski

8/21/201942 seconds
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September 2019 Summary Lewis

8/21/20191 minute, 6 seconds
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September 2019 Summary IIkezaki

8/21/20191 minute, 14 seconds
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Audio Summary September 2019

8/21/20199 minutes
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Patient-Based Real-Time Quality Control: Review and Recommendations

Analyzing quality control specimens is a daily component of modern medical laboratories to help assure that correct results are reported for patient samples.  That involves periodic analysis of materials with a known analyte concentration and estimating if measurement error is within acceptable criteria.  The QC samples may be obtained commercially or prepared within the laboratory.  However, how adequately do those samples represent authentic patient samples?  What if we were to use the patient samples themselves as a source of quality control data?  That’s not as radical as one might think as implementation and application of moving averages of patient results was suggested as early as the 1960s, but the practice never really caught on. A Review paper appearing in the August 2019 issue of Clinical Chemistry re-examines this concept and perhaps newer software can provide such data in near real-time, or as the paper calls it, patient-based real-time quality control. 
8/3/201916 minutes, 9 seconds
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The National Glycohemoglobin Standardization Program: Over 20 Years of Improving Hemoglobin A1c Measurement

Measurement of hemoglobin A1c in blood is essential for the management of patients with diabetes mellitus.  Hemoglobin A1c reflects the average blood glucose concentration over the proceeding 8 to 12 weeks.  While the clinical value of hemoglobin A1c was initially limited by large differences in results among various methods, the investment of considerable effort to implement standardization has brought about a marked improvement in analysis. In the July 2019 issue of Clinical Chemistry, a Review article chronicles the substantial progress that has been achieved in enhancing the accuracy of these measurements and the role of the National Glycohemoglobin Standardization Program in those efforts. 
8/2/201911 minutes, 41 seconds
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Noninvasive Detection of Bladder Cancer by Shallow-Depth Genome-Wide Bisulfite Sequencing of Urinary Cell-Free DNA for Methylation and Copy Number Profiling

Bladder cancer is the ninth most common cancer worldwide.  The current diagnosis and monitoring of bladder cancer is still heavily reliant on cystoscopy, an invasive procedure where the tumor is directly visualized.  However, a paper appearing in the July 2019 issue of Clinical Chemistry from Dr. Dennis Lo’s laboratory in Hong Kong reports that bladder cancer can be detected noninvasively in urinary cell-free DNA by methylomic and copy number analysis.  Such analyses could be used as a liquid biopsy to aid in the diagnosis and monitoring of bladder cancer.
8/2/201910 minutes, 12 seconds
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Audio Summary August 2019

7/18/20198 minutes, 42 seconds
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August 2019 Summary Chen

7/18/20191 minute
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August 2019 Summary Kuusisto

7/18/201955 seconds
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August 2019 Summary Carobene2

7/18/201953 seconds
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August 2019 Summary Hjort

7/18/20191 minute
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August 2019 Summary Sepiashvili

7/18/201955 seconds
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August 2019 Summary Body

7/18/201952 seconds
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August 2019 Summary Roraas

7/18/201943 seconds
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August 2019 Summary He

7/18/201956 seconds
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Audio Summary July 2019

6/20/20198 minutes, 40 seconds
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July 2019 Summary Cheng

6/20/201959 seconds
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July 2019 Summary JLi

6/20/201949 seconds
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July 2019 Summary Tan

6/20/201957 seconds
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July 2019 Summary Boeddinghaus

6/20/20191 minute, 4 seconds
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July 2019 Summary Vylegzhanina

6/20/201948 seconds
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03 Summary Lyngbakken

6/20/201959 seconds
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July 2019 Summary Whitman

6/20/20191 minute, 4 seconds
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July 2019 Summary Grammer

6/20/201957 seconds
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Low HDL Cholesterol and High Risk of Autoimmune Disease: Two Population-Based Cohort Studies Including 117341 Individuals

High-density lipoprotein or HDL is one of the most important of the lipoproteins in most species and there is evidence that points towards a role of HDL in normal immune function.  A paper appearing in the May 2019 issue of Clinical Chemistry tested the hypothesis that concentrations of HLD cholesterol are associated with risk of autoimmune disease. That study from the Copenhagen General Population Study and the Copenhagen City Heart Study included over 100,000 individuals.  We are pleased to have the senior author of that paper with us today, Dr. Borge Nordestgaard. 
6/17/20197 minutes, 10 seconds
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Inspiring Minds-: Patrick Bossuyt

6/5/201918 minutes, 53 seconds
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What the Protecting Access to Medicare Act Means for Clinical Laboratories

In 2014, the U.S. Congress passed the Protecting Access to Medicare Act (PAMA) which required CMS, the Centers for Medicare & Medicaid Services, to revise the clinical laboratory fee schedule to reflect private sector payment rates.  Many in the laboratory community believed that the cuts are too deep and have urged CMS to recalculate fees to more accurately reflect the spectrum of laboratories that provide testing for patients. To gain greater insight on the potential impact of PAMA, a Q&A feature in the June 2019 issue of Clinical Chemistry asked six experts with different roles in this field to give their opinions on several of the key issues pertaining to the new reimbursement rates, particularly its potential impact on the laboratory marketplace and patient care.
6/5/201917 minutes, 53 seconds
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A Message to Medical School Promotion Committees: Proper Credit for Peer-Reviewed Online Educational Materials

The standard course for academic promotion has primarily been based on the number and quality of peer-reviewed papers published and the number of peer-reviewed grants received.  Other academic activities such as teaching, book editing, mentoring, and service to scientific organizations have also been considered.  But this process often falls short for those whose interests are in biomedical education or in translational and clinical applications. In the June 2019 issue of Clinical Chemistry, Nader Rifai, Brian Smith, and Maureen Connelly published an editorial titled, “A Message to Medical School Promotion Committees:  Proper Credit for Peer-Reviewed Online Educational Materials.”
6/3/201911 minutes, 18 seconds
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Generation of Highly Biomimetic Quality Control Materials for Noninvasive Prenatal Testing Based on Enzymatic Digestion of Matched Mother–Child Cell Lines

Non-invasive prenatal testing based on cell-free DNA is now a widely used technique. However, quality control materials that have properties identical to clinical samples and that are applicable to a wide range of procedures are not available to support assay development, internal or external quality control, and proficiency testing. The June 2019 issue of Clinical Chemistry includes a study describing the development of such quality control materials that comprise simulated human plasma and mixtures of mother cell line derived cell-free DNA based on DNA fragmentation factor digestion.
6/3/201911 minutes, 2 seconds
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Audio Summary June 2019

5/22/20197 minutes, 55 seconds
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June 2019 Summary Li

5/22/201954 seconds
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June 2019 Summary Burkve

5/22/20191 minute, 3 seconds
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June 2019 Summary Zelniker

5/22/20191 minute, 13 seconds
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June 2019 Summary Matin

5/22/20191 minute, 7 seconds
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June 2019 Summary Zhang

5/22/20191 minute, 14 seconds
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June 2019 Summary Mendelian Randomization Group

5/22/20191 minute, 8 seconds
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Increased Clinical Sensitivity and Specificity of Plasma Protein N-Glycan Profiling for Diagnosing Congenital Disorders of Glycosylation by Use of Flow Injection–Electrospray Ionization–Quadrupole Time-of-Flight Mass Spectrometry

Inborn errors of metabolism are rare diseases in which a single gene defect causes a clinically significant block in a metabolic pathway resulting either in an accumulation of substrate behind the block or a lack or deficiency of the product.  Profiling metabolites in the pathway could allow for accurate and timely identification of patients who have these diseases and help physicians to devise effective treatment.   Congenital disorders of glycosylation represent one of the largest groups of such metabolic disorders.  In May 2019, Clinical Chemistry published a study on the development and validation of a plasma protein N-glycan assay using a flow injection-electrospray ionization-quadrupole time-of-flight mass spectrometry.
5/9/20198 minutes, 46 seconds
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Privacy in Direct-to-Consumer Genetic Testing

For about the past 15 years, the public has become aware that genetic testing can provide medical diagnosis and inform specific medical therapies.  In addition to being used for medical purposes, genetics has also become a popular recreational tool for genealogy and general wellness.  The number of customers for direct-to-consumer genetic testing has now grown to greater than 12 million and continues to rise. In the May 2019 issue of Clinical Chemistry, a Q&A feature titled “Privacy in Direct-to-Consumer Genetic Testing” asked four experts with different roles in this field to discuss the current state of genomics and privacy.
5/2/20199 minutes, 2 seconds
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Parameters for Validating a Hospital Pneumatic Tube System

Hospital pneumatic tube systems provide rapid transportation of patient samples to the laboratory.  However, it is known that the physical movement through a pneumatic tube system can agitate blood samples and sometimes cause cells to break open and leak intracellular components.  As a result, multiple laboratory results are susceptible to error. The May 2019 issue of Clinical Chemistry published a paper by Dr. Ann Gronowski and Dr. Christopher Farnsworth and their colleagues at the Washington University School of Medicine in St. Louis that describes parameters for evaluating a hospital pneumatic tube system. 
5/2/201911 minutes, 38 seconds
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May 2019 Summary Farnsworth

4/22/201955 seconds
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May 2019 Summary Hartley

4/22/20191 minute
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May 2019 Summary Sans

4/22/201954 seconds
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May 2019 Summary Yu

4/22/201946 seconds
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May 2019 Summary Chen

4/22/201954 seconds
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May 2019 Summary Madsen

4/22/201940 seconds
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Audio Summary May 2019

4/22/20196 minutes, 29 seconds
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Can Mendelian Randomization Shift into Reverse Gear?

Mendelian randomization is a genetic epidemiological approach that is made substantial inroads into our understanding of the causes and consequences of disease, but can that same technique be run in reverse? In the March 2019 issue of Clinical Chemistry, a paper investigated potential blood markers of early chronic kidney disease which are caused by loss of kidney function, using an innovative reverse Mendelian randomization approach. That same issue included an editorial that was authored by Dr. Michael Holmes from the University of Oxford and by Dr. George Davey Smith from the University of Bristol, both from the United Kingdom and both are our guests in this podcast.
4/17/201913 minutes, 28 seconds
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Challenges in the Assessment and Diagnosis of Polycystic Ovary Syndrome

Polycystic ovary syndrome is a complex endocrine-mediated disorder in women with an estimated prevalence of about 10%. Women with PCOS typically present with heterogeneous clinical signs and symptoms such as excess hair growth, irregular menstrual cycles, infertility and metabolic issues. Currently, there are no universal criteria for diagnosis of this condition and as a result, women with the disorder often reports significant delays in diagnosis and poor follow-up care. A Q&A feature in the March 2019 issue of Clinical Chemistry asked five experts with different roles in this field to discuss recent advances and ongoing challenges surrounding the current diagnostic criteria, available biomarkers, and the timely diagnosis and management of women with polycystic ovary syndrome.
4/17/201916 minutes, 11 seconds
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A Mendelian Randomization-Based Approach to Identify Early and Sensitive Diagnostic Biomarkers of Disease

Identifying markers of chronic kidney disease that occur early in the disease process and are specific to loss of kidney function may allow timely more accurate identification of patients who will eventually develop the disease. In the March 2019 issue of Clinical Chemistry, a paper investigated potential blood markers of early chronic kidney disease which are caused by loss of kidney function using an innovative reverse Mendelian randomization approach.
4/3/201911 minutes, 52 seconds
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Validation of a Metabolite Panel for a More Accurate Estimation of Glomerular Filtration Rate Using Quantitative LC-MS/MS

Glomerular filtration rate, or GFR, is generally accepted as the best overall index of kidney function, and a decrease in GFR has important implications for prognosis in patient management.  GFR is most commonly estimated by calculation, using the serum concentration of an endogenous filtration marker such as creatinine and demographic variables such as age, sex, and race.  In the March 2019 issue of Clinical Chemistry, a paper investigated the possibility of developing a more accurate estimate of GFR, using a panel of metabolites measured by quantitative liquid chromatography, tandem mass spectrometry without creatinine, cystatin C, or demographic variables.
3/26/20198 minutes, 33 seconds
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Which Methods for Determining Glomerular Filtration Rate Most Strongly Associate with Risk of Progression of Kidney Disease?

In the March 2019 issue of Clinical Chemistry, Professor Andrew Levey and others from a multinational consortium of institutions, published a study titled, “Validation of a Metabolite Panel for a More Accurate Estimation of Glomerular Filtration Rate Using Quantitative LC-MS-MS.”  In fact, we have another podcast from one of the authors of that paper available.  But today, we’re joined by Dr. Anders Berg, the Associate Medical Director of the Core Laboratories at Cedars-Sinai in Los Angeles, who co-authored an editorial that accompanied the paper. Dr. Berg is here to help us deconvolute the significance of this intriguing study and where this research might lead us in the future. 
3/26/20199 minutes, 48 seconds
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Audio Summary April 2019

3/25/20196 minutes, 55 seconds
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April 2019 Summary Jones

3/25/201948 seconds
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April 2019 Summary Yin

3/25/201959 seconds
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April 2019 Summary Jung

3/25/20191 minute, 1 second
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April 2019 Summary Franken

3/25/20191 minute, 2 seconds
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April 2019 Summary Fredsoe

3/25/201956 seconds
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April 2019 Summary Norman

3/25/201959 seconds
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Inflammatory Biomarkers and Clinical Judgment in the Emergency Diagnosis of Urgent Abdominal Pain

Acute abdominal pain is one of the most common presenting symptoms in emergency department patients and its differential diagnosis can be extensive and challenging.  Rapid and accurate diagnosis of urgent causes of abdominal pain is essential for the early initiation of effective therapy and efficient patient flow. To see how the clinical laboratory can help in this process, a recent study that appeared in the February 2019 issue of Clinical Chemistry examined inflammatory biomarkers in the emergency diagnosis of urgent abdominal pain.
3/19/201911 minutes, 11 seconds
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Type III Hyperlipoproteinemia: The Forgotten, Disregarded, Neglected, Overlooked, Ignored but Highly Atherogenic, and Highly Treatable Dyslipoproteinemia

Cardiovascular risk is so high in type III hyperlipoproteinemia that is typically a “treat immediately on diagnosis” disorder.  In the February 2019 issue of Clinical Chemistry, a paper presented the advantages of a non-high density lipoprotein cholesterol ratio with apolipoprotein B as a diagnostic tool for type III hyperlipoproteinemia.  In the same issue, an accompanying editorial entitled “Type III Hyperlipoproteinemia: The Forgotten, Disregarded, Neglected, Overlooked, Ignored but Highly Atherogenic, and Highly Treatable Dyslipo-proteinemia” was also published.  The author of that article is Dr. Allan Sniderman, the Edwards Professor of Cardiology at McGill University in Montreal, Quebec, Canada, and he is our guest in this podcast. 
2/25/201912 minutes, 41 seconds
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March 2019 Summary Anand

2/19/201959 seconds
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March 2019 Summary Braga

2/19/20191 minute
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March 2019 Summary Riethdorf

2/19/201954 seconds
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March 2019 Summary Rackow

2/19/201954 seconds
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March 2019 Summary Boeddinghaus

2/19/201958 seconds
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March 2019 Summary Mohammadi-Shemirani

2/19/201959 seconds
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March 2019 Summary Toes-Zoutendijk

2/19/201955 seconds
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March 2019 Summary Freed

2/19/201950 seconds
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Audio Summary March 2019

2/19/20198 minutes, 54 seconds
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Development and Validation of Apolipoprotein AI-Associated Lipoprotein Proteome Panel for the Prediction of Cholesterol Efflux Capacity and Coronary Artery Disease

High-density lipoproteins, or HDLs, are macromolecular assemblies that play a key role in lipid transport, but also exert effects in endothelial function, thrombosis, and inflammation.  A recent focus by several groups on HDL function rather than HDL cholesterol revealed that efflux capacity was inversely associated with coronary artery disease.  Traditionally, cholesterol efflux is measured using a cell-based assay where cultured microphages are treated with radioactively-labeled cholesterol and subsequently exposed to a cholesterol acceptor. A recent study that appeared in the February 2019 issue of Clinical Chemistry took a completely different approach and assessed the lipoprotein proteome by using liquid chromatography and tandem mass spectrometry to measure 21 lipoprotein associated proteins.
2/6/20196 minutes, 16 seconds
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Using Machine Learning to Aid the Interpretation of Urine Steroid Profiles

Machine learning involves the study of algorithms and mathematical models so that computer systems can progressively improve their performance on solving a specific task. A recent application of machine learning through the interpretation of urine steroid profiles appears in the November 2018 issue of Clinical Chemistry.
2/4/201916 minutes, 26 seconds
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Audio Summary February 2019

1/24/20199 minutes, 29 seconds
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February 2019 Summary Wood

1/24/20191 minute, 2 seconds
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February 2019 Summary Hansen

1/24/20191 minute, 4 seconds
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February 2019 Summary Boot

1/24/20191 minute, 5 seconds
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February 2019 Summary Breidthardt

1/24/20191 minute, 5 seconds
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February 2019 Summary Salameh

1/24/20191 minute, 6 seconds
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February 2019 Summary Jin

1/24/20191 minute, 1 second
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February 2019 Summary Kong

1/24/20191 minute, 12 seconds
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February 2019 Summary Myrick

1/24/201942 seconds
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Assessment of Digital PCR as a Primary Reference Measurement Procedure to Support Advances in Precision Medicine

A recent study appears in the September 2018 issue of Clinical Chemistry, an assessment of digital PCR as a primary reference measurement procedure to support advances in precision medicine.
1/22/20199 minutes, 29 seconds
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Differences in Health Outcomes between Men and Women: Biological, Behavioral, and Societal Factors

In almost all high-income countries, the U.S. included, women live longer than men.  As we enter a new era in which biomedical data are increasingly ubiquitous, current and future research may help us understand the fundamental issues that drive differences in longevity and other health outcomes between men and women.  Our ability to measure the entire spectrum of information about the human biological, environmental, and behavioral condition will become both routine and relatively inexpensive from genes to genomics.  From clinical data to the electronic health record in insurance claims.  From digital information about behavior and social interaction to geospatial referencing. The January 2019 issue of Clinical Chemistry is devoted to topics of men’s health and in that issue, an article examined differences in health outcomes between men and women and underlying biological, behavioral, and societal factors. 
1/7/201914 minutes, 13 seconds
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Male Contraceptive Development: Update on Novel Hormonal and Nonhormonal Methods

The unintended pregnancy rate in the United States is approximately 45% despite of variety of contraceptive options available to women.  Today, male condoms and withdrawal are the only reversible contraceptive methods available to men with typical failure rates between 10% and 20%.  But what about a male contraceptive pill?  Studies indicate that more than half of men would be interested in using a reversible method if it was available.  And many women would be willing to rely on their partner to use such a contraceptive.  Unplanned pregnancy rates could improve if both partners use a contraceptive method or if men had more options to control their own fertility. The January 2019 issue of Clinical Chemistry is devoted to topics of men’s health.  And in that issue, an article examined developments in male contraceptives with an update on both novel hormonal and non-hormonal methods.
1/2/201912 minutes, 13 seconds
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Redesigning Prostate Cancer Screening Strategies to Reduce Overdiagnosis

The January 2019 issue of Clinical Chemistry is devoted to the area of men’s health.  Aside from non-melanoma skin cancer, prostate cancer is the most common cancer among men in the United States.  It is remarkable that even though the introduction of prostate specific antigen, or PSA, into U.S. clinical practice occurred over three decades ago, researchers and clinicians are still debating its value for prostate cancer screening. In that special issue, Dr. Andrew Vickers published an Opinion article titled, “Redesigning Prostate Cancer Screening Strategies to Reduce Overdiagnosis.”
1/2/201914 minutes, 29 seconds
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Updates on Screening, Prevention, Treatment, and Genetic Markers for Preeclampsia

Preeclampsia is a serious disorder of pregnancy that occurs by 24 weeks of gestation or later and can occur in up to 5% of all pregnancies.  Maternal weight, advancing maternal age, nulliparity, a previous pregnancy with preeclampsia, and insulin-dependent diabetes are just some of the predisposing factors for developing preeclampsia.  Over the past few years, development in screening, prevention, and treatment for preeclampsia have occurred and genetic studies are just now providing new insights into the etiology of this disorder. A Q&A feature in the December 2018 issue of Clinical Chemistry asked three experts with different roles in this field to discuss recent advances and ongoing challenges in preeclampsia research and implementation. 
12/21/201818 minutes, 33 seconds
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CRISPR Methods for Nucleic Acid Detection Herald the Future of Molecular Diagnostics

The December 2018 issue of Clinical Chemistry published a Perspective article titled “CRISPR Methods for Nucleic Acid Detection Herald the Future of Molecular Diagnostics,” which discusses the findings of Chen and colleagues who earlier this year were the first to create a diagnostic tool out of a CRISPR-Cas system and demonstrate its clinical utility. 
12/20/201811 minutes, 1 second
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Audio Summary January 2019

12/19/20186 minutes, 39 seconds
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January 2019 Summary Winston-McPherson

12/19/201857 seconds
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January 2019 Summary Marrone

12/19/201856 seconds
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January 2019 Summary Srinivasan

12/19/201858 seconds
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January 2019 Summary Mikkelsen

12/19/201858 seconds
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January 2019 Summary SoRelle

12/19/201847 seconds
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January 2019 Summary Jayasena

12/19/201856 seconds
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Direct Comparison of Cardiac Troponin T and I using a Uniform and a Sex-specific Approach in the Detection of Functionally Relevant Coronary Artery Disease

Coronary artery disease is the most common cause of death and morbidity in developed countries, resulting in substantial healthcare costs.  The early detection of this disease and identification of patients at risk for myocardial infarction has enormous medical and economic value.  The introduction of high-sensitivity cardiac troponin assays has revolutionized early diagnosis of myocardial infarction, but what about their use for identifying patients at risk with stable coronary artery disease?Coronary artery disease is the most common cause of death and morbidity in developed countries, resulting in substantial healthcare costs.  The early detection of this disease and identification of patients at risk for myocardial infarction has enormous medical and economic value.  The introduction of high-sensitivity cardiac troponin assays has revolutionized early diagnosis of myocardial infarction, but what about their use for identifying patients at risk with stable coronary artery disease? A recent study to address that issue as well as a comparison of high-sensitivity cardiac troponin I and troponin T assays appeared in the November 2018 issue of Clinical Chemistry.
12/5/201811 minutes, 49 seconds
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Influence of Molecular Testing on Influenza Diagnosis

Influenza viruses affect millions of people each year, leading to several hundred thousand hospitalizations and thousands of deaths annually in the U.S. Early antiviral therapy reduces the duration of illness, complications and mortality associated with the flu, yet antivirals are often underutilized. However, patients with a positive influenza diagnostic test results are more likely to receive appropriate antiviral therapy and less likely to be prescribed unnecessary antibiotics. Access to reliable influenza testing is critical to facilitate both optimal patient outcomes and antimicrobial stewardship. Recently, the first point-of-care molecular diagnostic test was cleared by the U.S. Food and Drug Administration for detection of the flu. At the same time, concerns about the performance of commonly used rapid antigen tests have surfaced. The landscape of influenza diagnostics is rapidly evolving and clinical laboratories are certain to face pressure regarding new testing modalities. A Q&A feature in the November 2018 issue of Clinical Chemistry asked five experts with different roles in this field to discuss recent advances and ongoing challenges in influenza diagnostics.
12/5/201810 minutes, 23 seconds
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Decmeber 2018 Summary Huang

11/20/20181 minute, 5 seconds
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December 2018 Summary Kiehl

11/20/201846 seconds
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December 2018 Summary Folch

11/20/201844 seconds
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December 2018 Summary Liu

11/20/20181 minute, 1 second
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December 2018 Summary Milosevic

11/20/201854 seconds
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December 2018 Summary Hejl

11/20/20181 minute, 2 seconds
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December 2018 Summary Smith

11/20/201859 seconds
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December 2018 Summary Neumann

11/20/201849 seconds
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Audio Summary December 2018

11/20/20188 minutes, 16 seconds
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Audio Summary November 2018

10/23/20189 minutes, 30 seconds
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November 2018 Summary Parker

10/23/201857 seconds
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November 2018 Summary Eisenhofer

10/23/201859 seconds
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November 2018 Summary Sharma

10/23/20181 minute, 13 seconds
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November 2018 Summary Mansukhani

10/23/20181 minute, 6 seconds
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November 2018 Summary Ibrahim

10/23/20181 minute, 14 seconds
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November 2018 Summary Welsh

10/23/201854 seconds
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November 2018 Summary Mueller

10/23/201854 seconds
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November 2018 Summary Wilkes

10/23/201850 seconds
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Current and Emerging Trends in Point-of-Care Technology and Strategies for Clinical Validation and Implementation

A Review appearing in the October 2018 issue of Clinical Chemistry describes the trends and the technologies that have emerged in the past decade and are shaping contemporary point-of-care testing.  We are joined by the Review’s first author, Dr. Ping Wang.  She is Associate Professor of Pathology and Laboratory Medicine at the University of Pennsylvania, and Director of Clinical Chemistry and Core Laboratory at the Hospital of the University of Pennsylvania. 
10/18/201810 minutes, 21 seconds
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Commutability Assessment of Candidate Reference Materials for Pancreatic α-Amylase

Standardization efforts for amylase have been limited by a lack of commutability in the current certified reference material.  New candidate reference materials have been developed to potentially overcome this limitation, facilitating their use for calibration and trueness control of routine measurement systems.  An original article appearing in the August of 2018 issue of Clinical Chemistry describes the commutability assessments of these new candidate reference materials for pancreatic alpha amylase.  The authors investigated if a reference material with an artificial matrix could be commutable for routine methods and compared to different statistical approaches to assess the commutability of the candidate reference materials.
10/16/201811 minutes, 4 seconds
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October 2018 Summary Zavridou

9/21/20181 minute, 5 seconds
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October 2018 Summary Li

9/21/20181 minute, 5 seconds
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October 2018 Summary Joosse

9/21/201852 seconds
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October 2018 Summary Lee

9/21/201858 seconds
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October 2018 Summary Delatour

9/21/20181 minute, 14 seconds
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October 2018 Summary Beck

9/21/201857 seconds
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October 2018 Summary Kabe

9/21/201855 seconds
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October 2018 Summary Andini

9/21/20181 minute, 9 seconds
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Audio Summary October 2018

9/21/20189 minutes, 39 seconds
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September 2018 Summary Wald

8/8/201848 seconds
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September 2018 Summary Aarsand

8/8/20181 minute, 5 seconds
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September 2018 Summary Myhre

8/8/20181 minute, 23 seconds
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September 2018 Summary Caironi

8/8/201846 seconds
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September 2018 Summary Boeddinghaus

8/8/20181 minute, 17 seconds
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September 2018 Summary Silva

8/8/201853 seconds
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September 2018 Summary Guo

8/8/201848 seconds
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September 2018 Summary Darebna

8/8/20181 minute, 2 seconds
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Audio Summary September 2018

8/8/20189 minutes, 1 second
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Validation of an Expanded Carrier Screen that Optimizes Sensitivity via Full-Exon Sequencing and Panel-wide Copy Number Variant Identification

Previously, carrier screening has been used in at-risk and specific ethnic populations, or for a limited number of diseases.  The broader screening approach, called expanded carrier screening, was endorsed in 2017 by the American College of Obstetricians and Gynecologists as an acceptable strategy for preconception and prenatal carrier screening.  This is expected to have a significant clinical impact, as targeted screening approaches likely have missed a large number of affected pregnancies due to the limited conditions tested, the unknown ethnic ancestry of many individuals, and the fact that the majority of children born with a genetic disease have no family history of the condition. When provided with results that their pregnancy is at-risk for a severe condition, study show approximately 80% of such couples pursue alternative reproductive options.  Given these consequences and the relative rarity of the included diseases, expanded carrier screening panels must have a high detection rate to correctly identify at-risk couples, and to minimize the residual risk when only one partner is tested positive.  Though next generation sequencing has revolutionized genetic testing as a whole, advances in detecting copy number variants by next generation sequencing have been critical to improving the analytical quality of expanded carrier screening panels. An original research article appearing in the July 2018 issue of Clinical Chemistry describes these achievements while establishing the analytical validity of expanded carrier screening and quantifying how many pregnancies could be impacted by this approach in the general U.S. population.
8/2/201815 minutes, 43 seconds
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Toward a Global Overview of HbA1c Test Performance

Test standardization is a complex and multifaceted effort that’s remained a major goal for laboratory medicine’s professional societies, including the AACC and IFCC.  Hemoglobin A1c is an example of a test that has undergone global standardization due to its worldwide importance in diabetes screening, diagnosis, and care.  A key part of any standardization effort is aligning all manufacturers’ methods to an accepted reference method.  This was the main aim of the European Hemoglobin A1c trial devised by the IFCC’s Committee on Education and Use of Biomarkers in Diabetes. The August 2018 issue of Clinical Chemistry includes an original research article that describes the European Hemoglobin A1c trial and its results.
7/31/201814 minutes, 57 seconds
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Preanalytical Processing and Biobanking Procedures of Biological Samples for Metabolomics Research

Proper handling during the collection and processing of specimens is crucial for producing reliable results.  These pre-analytical steps are highly vulnerable to the introduction of experimental bias and variance, which may drastically degrade specimen quality.  Analytes are affected differently by uncontrolled variations in specimen collection, processing, and storage protocols. The August 2018 issue of Clinical Chemistry includes a Review article from the Precision Medicine and Pharmacometabolomics Task Group of the Metabolomics Society.  The authors summarize the current variability among pre-analytical processing and biobanking procedures for metabolomics research and provide recommendations for best practices.
7/30/201812 minutes, 49 seconds
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Association of Tryptophan Metabolites with Incident Type 2 Diabetes in the PREDIMED Trial: A Case–Cohort Study

According to the World Health Organization, type 2 diabetes comprises the majority of the more than 400 million people with diabetes around the world. In efforts to better understand the disease, metabolomics measurements have identified tryptophan metabolites as potential biological mediators in the development of type 2 diabetes.  The PREDIMED trial was a multi-center trial of Mediterranean-style diets where the primary prevention of cardiovascular events that also included type 2 diabetes as a secondary end point. An original research article published in the August 2018 issue of Clinical Chemistry studies whether tryptophan metabolites in PREDIMED participants were associated with development of type 2 diabetes and insulin resistance.
7/30/20187 minutes, 43 seconds
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Characterization of Human Salivary Extracellular RNA by Next-generation Sequencing

It was once thought that RNA only existed within cells.  However, recent discoveries show that RNA is exported from cells and may be functional in cell to cell communication.  This extracellular RNA can be abundant and stable and is found in bodily fluids such as saliva.  Saliva has long been of interest for biomarker discovery and development, given its noninvasiveness and relative ease of collection. Despite these advantages, use of saliva for understanding more about extracellular RNA and its role in human biology and disease through RNA sequencing has some challenges.  The high bacterial content and low abundance of extracellular RNA in saliva mean that optimization of RNA library construction and isolation protocols are critical to the success of these RNA sequencing experiments. An original research article in the July 2018 issue of Clinical Chemistry compares different RNA isolation methods and library construction kits for long and small RNA sequencing of salivary extracellular RNA.  The authors described which protocols provide the best RNA yield and detection by next generation sequencing.
7/28/201813 minutes, 9 seconds
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RNA Profiles of Circulating Tumor Cells and Extracellular Vesicles for Therapy Stratification of Metastatic Breast Cancer Patients

Liquid biopsy methods are an exciting area in cancer screening, diagnosis, and management.  These methods are aimed at measuring genomic signatures of tumor cells in the blood.  They rely on detection of circulating tumor cells, tumor-derived extracellular vesicles, or exosomes, or cell-free circulating DNA.  Liquid biopsy methods have been proposed to compliment, enhance or perhaps even replace tissue biopsy in some scenarios.  Liquid biopsy methods are an exciting area in cancer screening, diagnosis, and management.  These methods are aimed at measuring genomic signatures of tumor cells in the blood.  They rely on detection of circulating tumor cells, tumor-derived extracellular vesicles, or exosomes, or cell-free circulating DNA.  Liquid biopsy methods have been proposed to compliment, enhance or perhaps even replace tissue biopsy in some scenarios.   Beyond the minimal invasiveness, performing liquid biopsy may overcome some other weaknesses of tissue biopsy that are related to a single site’s inability to capture intratumor heterogeneity or be representative of all the changes within the tumor.  All three approaches to detect a tumor’s genetic material have been applied for therapy stratification and monitoring in breast cancer.  However, knowledge about the differences between these methods in the same patient cohort is limited. An original article in the July 2018 issue of Clinical Chemistry compared messenger RNA profiles of circulating tumor cells and extracellular vesicles in patients with metastatic breast cancer to estimate the utility of each in therapy management.
7/27/20187 minutes, 35 seconds
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Quantifying Atherogenic Lipoproteins: Current and Future Challenges in the Era of Personalized Medicine and Very Low Concentrations of LDL Cholesterol. A Consensus Statement from EAS and EFLM

Laboratory testing is a key component of models of care for all types of lipid disorders.  New therapies demand accuracy of dyslipidemia testing at very low LDL cholesterol concentration ranges.  Inaccurate results lead to incorrect diagnosis and therapeutic management, both of which are costly to society and harmful to patients. To address these key issues of lipoprotein and apolipoprotein markers and reach consensus on contemporary lipid testing, a multidisciplinary panel was established by the European Federation of Clinical Chemistry and Laboratory Medicine and the European Atherosclerosis Society.       The July 2018 issue of Clinical Chemistry includes a special report from this joint consensus initiative.  It provides recommendations for improving the use of the lipid profile to assess cardiovascular disease risk conferred by atherogenic lipoproteins.  
7/26/201814 minutes, 17 seconds
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Audio Summary August 2018

7/23/20187 minutes, 58 seconds
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August 2018 Summary Gai

7/23/201853 seconds
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August 2018 Summary Kim

7/23/20181 minute, 8 seconds
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August 2018 Summary Cannaert

7/23/201851 seconds
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August 2018 Summary Yu

7/23/201836 seconds
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August 2018 Summary Tobias

7/23/20181 minute, 10 seconds
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August 2018 Summary Deprez

7/23/201851 seconds
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August 2018 Summary EurA1C Group

7/23/20181 minute, 10 seconds
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Full-Size Cardiac Troponin I and Its Proteolytic Fragments in Blood of Patients with Acute Myocardial Infarction: Antibody Selection for Assay Development

Cardiac troponins are recognized as the primary biomarkers used in the diagnosis of acute myocardial infarction.  These are commonly detected by immunoassays, which offer the wide availability and rapid results needed in clinical care scenarios to rule out myocardial infarction.  However, immunodetection methods can be limited when there is degradation of the target analyte, as is the case with cardiac troponin I. Early studies suggested that the central region, located between amino acid residues 30 and 110, was the most stable part of the molecule.  This was a driver for the majority of current diagnostic systems to utilize antibodies with epitopes located within the central region.  A down side to this approach is that auto antibodies also bind to this region, potentially causing interference and erroneous results. As more recent studies have identified additional cardiac troponin I proteolytic fragments, it is important to understand their composition and prevalence in the blood of patients with acute myocardial infarction.  Furthermore, there is a need to evaluate whether monoclonal antibodies specific to the regions beyond the central section of the molecule can be used to develop cardiac troponin I assays with improved performance over those currently available.  An original article appearing in the July 2018 issue of Clinical Chemistry describes a study investigating these concepts. 
7/19/201811 minutes, 37 seconds
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Audio Summary July 2018

6/26/20189 minutes, 24 seconds
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July 2018 Summary Rasmussen

6/26/201853 seconds
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July 2018 Summary Katrukha

6/26/20181 minute, 7 seconds
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July 2018 Summary Alimohamed

6/26/20181 minute
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July 2018 Summary Li

6/26/201852 seconds
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July 2018 Summary Ludwig

6/26/201858 seconds
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July 2018 Summary Hogan

6/26/20181 minute, 1 second
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July 2018 Summary Keup

6/26/20181 minute, 16 seconds
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July 2018 Summary Than

6/26/201859 seconds
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Clinical Utility of Transcriptome Sequencing: Toward a Better Diagnosis for Mendelian Disorders

The June 2018 issue of Clinical Chemistry published a Perspective article titled, “Clinical Utility of Transcriptome Sequencing: Towards a Better Diagnosis for Mendelian Disorders.”  In it, the authors Drs. Sumant Chakravorty and Madhuri Hegde discussed the impact of findings by Cummings and colleagues, who have shown for the first time in 2017 the clinical utility of next generation sequencing based transcriptome sequencing to increase molecular diagnostic yield by sequencing RNA.
5/29/201811 minutes, 23 seconds
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Neurogranin as Cerebrospinal Fluid Biomarker for Alzheimer Disease: An Assay Comparison Study

Dementia is a devastating disease with a huge global impact. Today, over 46 million people live with dementia worldwide. It is possible that promising treatments for Alzheimer’s disease have failed thus far because treatment was started at a late stage of the disease process. So, early treatment could be beneficial, but how do you recognize a patient with Alzheimer’s disease if he or she does not present with clinical symptoms? This is a key question, and presents an enormous opportunity for laboratory test based on biological markers of ongoing brain pathology. One example is measurement of neurogranin, a protein in cerebrospinal fluid that correlates with cognitive decline and is a potential novel biomarker for Alzheimer’s disease dementia. There are multiple antibody-based neurogranin assays, which all target different segments of the protein. An original article in the June 2018 issue of Clinical Chemistry compared the analytical and the diagnostic performance of three commonly used neurogranin assays on the same cohort of patients.
5/29/20189 minutes, 24 seconds
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Clinical Evaluation of a Blood Assay to Diagnose Paucibacillary Tuberculosis via Bacterial Antigens

Tuberculosis is a prevalent and potentially deadly infectious disease with a worldwide incidence of more than 10 million new cases each year, and in 2015 alone, 1.4 million deaths were attributed to TB.  Much of the world relies on microbiologic techniques to detect tuberculosis including smear microscopy and mycobacterial culture which has a very long sample to answer timeframe. Both methods also have low clinical sensitivity for paucibacillary TB cases which account for more than 60% of new TB cases each year in emerging endemic areas such as China.  PCR-based methods are faster but also have limitations.  In the May 2018 issue of Clinical Chemistry, Dr. Tony Hu and his colleagues described a new peptide-based approach to detecting TB that overcomes those issues by using antibody conjugated nanoparticles, or nanodiscs, to bind specific peptides present in digested serum samples from cases of tuberculosis.  These are then detected by benchtop matrix-assisted laser desorption ionization-time-of-flight mass spectrometry, or MALDI-TOF MS.
5/17/201811 minutes, 54 seconds
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Audio Summary June 2018

5/16/20186 minutes, 12 seconds
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June 2018 Summary Schirmer

5/16/201851 seconds
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June 2018 Summary Waalkes

5/16/20181 minute, 6 seconds
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June 2018 Summary Wu

5/16/201857 seconds
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June 2018 Summary Willemse

5/16/201840 seconds
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June 2018 Summary Cannaert

5/16/201851 seconds
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June 2018 Summary Ismail

5/16/201849 seconds
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Clinical and Genomic Insights from Metastatic Cancers

Metastatic or recurrent cancers are a common cause of death among cancer patients.  Genomic investigations of cancer over the past 10 years have led to tremendous advancement in the understanding of the underlying biology and in the development of targeted treatment options.  Much of the discovery has been focused on primary treatment-naive cancers, since biological specimens for genomic analysis are more abundant in these cases that are available from patients with metastatic cancers. The lack of specimens has provided a significant obstacle in the genomic study of metastatic cancers.  Therefore, a recent study by Dr. Dan Robinson and colleagues reported in the journal Nature has raised excitement.  The investigators performed integrative analysis of DNA and RNA data for 500 metastatic lesions obtained from cancer patients with over 30 different primary cancer diagnoses to derive several types of molecular characterizations.  Though further studies are needed, applying these approaches to individual patients in the clinical setting of metastasis may lead to more precisely tailored therapeutic treatments with higher effectiveness. The May 2018 issue of Clinical Chemistry includes an expert commentary on this noteworthy study. 
5/10/201814 minutes, 47 seconds
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From DESI to the MasSpec Pen: Ambient Ionization Mass Spectrometry for Tissue Analysis and Intrasurgical Cancer Diagnosis

Surgery is a common form of treatment in patients with solid tumors.  Depending on the cancer, surgery may be used to remove cancer that is contained within a specific area, to debulk a tumor, or to ease symptoms caused by a tumor.  In each case, it is critical to distinguish cancerous from noncancerous tissue during the operation. Today, pathologists perform rapid microscopic investigations of biopsied tissue and make the diagnosis.  In the future, this may be conducted using mass spectrometry based measurements.  Ambient ionization mass spectrometry methods make localized molecular level chemical analysis of tissue samples possible at atmospheric pressure and without prior sample purification and pre-treatment.  Over the past decades, scientific advances have extended the role of these methods from the research laboratory to the operating room.  The April 2018 issue of Clinical Chemistry includes a commentary on the different technologies and their potential in cancer diagnosis.
5/9/201816 minutes, 55 seconds
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Evaluating Rapid Rule-out of Acute Myocardial Infarction Using a High-Sensitivity Cardiac Troponin I Assay at Presentation

Cardiac troponin measured with newer generations of analytically highly sensitive assays have been investigated in accelerated discharge protocols on the assumption that they have an improved ability to detect and quantify cardiomyocyte injury more quickly than previous generation’s assays. In the May 2018 issue of Clinical Chemistry, Dr. Jaimi Greenslade from the Royal Brisbane and Women’s Hospital in Herston, Australia, and her colleagues investigated a high sensitivity cardiac troponin I assay to see if a single test threshold can safely rule out acute MI.
5/1/201810 minutes, 23 seconds
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Audio Summary May 2018

4/24/20187 minutes, 44 seconds
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May 2018 Summary Hilderink

4/24/201858 seconds
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May 2018 Summary Selvin

4/24/201846 seconds
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May 2018 Summary Haselmann

4/24/20181 minute, 1 second
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May 2018 Summary Greenslade

4/24/20181 minute
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May 2018 Summary X Liu

4/24/20181 minute, 3 seconds
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May 2018 Summary Rejali

4/24/201854 seconds
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May 2018 Summary C Liu

4/24/20181 minute
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Advanced Whole-Genome Sequencing and Analysis of Fetal Genomes from Amniotic Fluid

Amniocentesis is a common procedure usually performed to collect cells from the fetus to allow testing for abnormal chromosomes.  Cells from the amniotic fluid are collected through centrifugation, cultured, and after about two weeks, analyzed by fluorescent in situ hybridization, or FISH, or with a microarray to detect abnormal chromosome copy number changes or large chromosomal structural rearrangements.  These tests have become the gold standard for detecting Down Syndrome and several other serious birth defects because they have a low false positive rate.  However, they are unable to detect the majority of birth defects.Amniocentesis is a common procedure usually performed to collect cells from the fetus to allow testing for abnormal chromosomes.  Cells from the amniotic fluid are collected through centrifugation, cultured, and after about two weeks, analyzed by fluorescent in situ hybridization, or FISH, or with a microarray to detect abnormal chromosome copy number changes or large chromosomal structural rearrangements.  These tests have become the gold standard for detecting Down Syndrome and several other serious birth defects because they have a low false positive rate.  However, they are unable to detect the majority of birth defects. In the April 2018 issue of Clinical Chemistry, a paper demonstrated the feasibility of generating an accurate whole genome sequence of a fetus from either the cellular or cell-free DNA of an amniotic sample. 
4/2/20187 minutes, 13 seconds
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Comparing Measures of HDL: On the Right Path with the Wrong Map

High-density lipoprotein, or HDL, cholesterol is often referred to as the “good” cholesterol due to its involvement in transport of cholesterol from peripheral tissues back to the liver during reverse cholesterol transport.  However, some studies have demonstrated that the size and composition of HTL particles may influence the mechanism and capacity for this cholesterol efflux and other possible protective activities.  So, some researchers believe that measurement of HTL particles, size, and composition may provide an indication of HTL protective activity. The March 2018 issue of Clinical Chemistry published a report comparing five different procedures for HDL particle measurement.  In the same issue an editorial by Dr. Jeffrey Meeusen appeared titled “Comparing Measures of HDL: On the Right Path with the Wrong Map.”
3/29/20187 minutes, 51 seconds
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The Biological Variation Data Critical Appraisal Checklist: A Standard for Evaluating Studies on Biological Variation

Data on biological variation can be used in many ways, most notably for the diagnosis and monitoring of disease and also for setting analytical performance specifications.  In 2014, after the first strategic conference of the European Federation of Clinical Chemistry and Laboratory Medicine, a task force was established to develop criteria for evaluation of the literature on biological variation. A report from this group appears in the March 2018 issue of Clinical Chemistry.
3/28/20189 minutes, 55 seconds
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Audio Summary April 2018

3/22/20187 minutes, 12 seconds
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April 2018 Summary vanderGugten

3/22/20181 minute, 2 seconds
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April 2018 Summary Kim

3/22/201855 seconds
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April 2018 Summary Mao

3/22/201838 seconds
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April 2018 Summary Amr

3/22/20181 minute, 5 seconds
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April 2018 Summary Topbas

3/22/20181 minute, 9 seconds
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April 2018 Summary Masi

3/22/201822 seconds
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April 2018 Summary deRond

3/22/201852 seconds
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Non-invasive Detection of Cocaine and Heroin Use Using Single Fingerprints

Studies have found that drugs are now so prevalent that over 10% of a population were found to have traces of Class A drugs on their fingerprints despite never using any of those drugs.  While fingerprints have been suggested as a possible sample to rapidly and noninvasively carry out drug testing, identifying drug users and not those who may have passively encountered a drug can be complex. In a study published in the June 2018 issue of Clinical Chemistry and available online now, researchers from the University of Surrey together with Dutch scientists may have found a solution.  They present evidence that their techniques give a zero false positive rate in the fingerprints from drug-free volunteers.  Yet, they were able to detect 87.5% of the cocaine users and 100% of heroin users.
3/22/20188 minutes, 11 seconds
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Imputation of Baseline LDL Cholesterol Concentration in Patients with Familial Hypercholesterolemia on Statins or Ezetimibe

Familial hypercholesterolemia is a frequent genetic disorder encountered in clinical practice and is associated with high levels of serum LDL cholesterol known as the “bad cholesterol.” A diagnosis of familial hypercholesterolemia has important clinical implications with respect to risk of cardiovascular disease and a requirement for intensive pharmacological therapy. Often, the baseline LDL cholesterol before treatment is not available because the patient has initiated and continues on lipid lowering therapy, especially statins. Furthermore, the original baseline LDL cholesterol may predate the current status by many years and cannot be easily retrieved. The February 2018 issue of Clinical Chemistry published a paper describing a method to obtain an imputed, or estimated, baseline LDL cholesterol concentration in these patients who are already taking cholesterol lowering drugs. Both a computer program and a smartphone app are available from links in the paper. We are pleased to have the lead author of that paper as a guest on this podcast. Isabelle Ruel is a Clinical Biochemist at the Research Institute of the McGill University Health Center Royal Victoria Hospital in Montreal, Quebec, and is currently the national coordinator of the Canadian Registry on Familial Hypercholesterolemia.
3/8/20184 minutes, 52 seconds
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Plasma Steroid Metabolome Profiling for Diagnosis and Subtyping Patients with Cushing Syndrome

A paper in the March 2018 issue of Clinical Chemistry describes a liquid chromatography tandem mass spectrometry procedure for multi-steroid profiling in Cushing syndrome and other disorders.  The lead author of that study is Dr. Graeme Eisenhofer, who is professor and the Chief of the Division of Clinical Neurochemistry at the Institute for Clinical Chemistry and Laboratory Medicine at the Technical University in Dresden, Germany.  He is our guest in this podcast.
3/7/20189 minutes, 50 seconds
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IFCC Working Group Recommendations for Assessing Commutability

Commutability is an important concept in determining traceability and the relationship among different methods that are used to measure the same analyte but just how to describe and classify commutable materials is not always straightforward.  The March 2018 issue of Clinical Chemistry published a series of three reports from the Working Group on Commutability of the International Federation of Clinical Chemistry and Laboratory Medicine, or IFCC, that describe recommendations for assessing commutability.
2/28/20189 minutes, 21 seconds
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Evaluation of Thyroid Function during Pregnancy: Have We Taken a Wrong Turn?

Normal pregnancy is associated with profound hormonal and metabolic changes in the mother, including changes in thyroid hormones.  These normal changes include increased thyroid binding globulin, increased total T3 and total T4, transient decrease in TSH, and in some patients, a transient increase in serum FT4 during the first trimester. In 2017, the American Thyroid Association issued new guidelines for the diagnosis and management of thyroid diseases during pregnancy, which can be difficult due to the numerous normal physiological changes.  Most would agree that the document is an excellent review of current literature relating to the assessment of thyroid status during pregnancy.  However, one researcher wonders if a particular recommendation of the document is misguided.
2/28/201811 minutes, 16 seconds
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March 2018 Summary Badertscher

2/27/20181 minute, 1 second
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Audio Summary March 2018

2/27/201812 minutes, 35 seconds
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March 2018 Summary Pavo

2/27/20181 minute, 13 seconds
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March 2018 Summary Eisenhofer

2/27/201858 seconds
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March 2018 Summary Tijms

2/27/201855 seconds
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March 2018 Summary Frobel

2/27/201850 seconds
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March 2018 Summary Kim

2/27/20181 minute, 3 seconds
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March 2018 Summary Greiner

2/27/20181 minute, 1 second
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March 2018 Summary El Heliebi

2/27/20181 minute, 7 seconds
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March 2018 Summary Zhou

2/27/20181 minute, 16 seconds
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March 2018 Summary Aarsand

2/27/20181 minute, 3 seconds
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March 2018 Summary Matera

2/27/20181 minute, 8 seconds
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Audio Summary February 2018

1/17/201812 minutes, 51 seconds
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February 2018 Summary Walter

1/17/201857 seconds
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February 2018 Summary Moen

1/17/20181 minute, 9 seconds
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February 2018 Summary Prickett

1/17/20181 minute, 1 second
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February 2018 Summary Ruel

1/17/201852 seconds
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February 2018 Summary Hill

1/17/20181 minute, 4 seconds
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February 2018 Summary Camunas-Soler

1/17/201853 seconds
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February 2018 Summary Dharajiya

1/17/201853 seconds
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February 2018 Summary Decraene

1/17/201852 seconds
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February 2018 Summary Aalipour

1/17/20181 minute
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February 2018 Summary Markou

1/17/20181 minute, 2 seconds
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February 2018 Summary Westgard

1/17/201859 seconds
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February 2018 Summary Farrokhi

1/17/20181 minute, 1 second
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Dietary Fat: Friend or Foe?

Expert Q&A 
1/3/201815 minutes, 16 seconds
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Use of Metabolomics in Improving Assessment of Dietary Intake

In the era of precision medicine, it is no surprise that there is an emerging interest in personalizing one’s diet based on their genetic make-up.  This concept called, “precision nutrition,” refers to the application of -omics techniques such as genomics and metabolomics, to achieve better prevention or treatment of disease to optimize nutrition. The January 2017 Clinical Chemistry special issue on obesity includes a review article that summarizes the current and future roles of nutritional metabolomics.
1/3/20188 minutes, 45 seconds
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Bad Breaks and Metabolic Effects of Bariatric Surgery

Although bariatric surgery techniques were originally developed to treat morbid obesity, the indications for their use have expanded as a consequence of the emerging evidence of their wider metabolic benefits.  Now described as metabolic surgeries, these treatment methods derive as much benefit from their metabolic and cardiovascular effects as from the resulting weight reduction. Though promising evidence is emerging, there is a need for further mechanistic studies to assess the true potential of metabolic surgery to treat the myriad other disorders of metabolism and to better understand their consequences in terms of cardiovascular disease and cancer risk reduction.  Additionally, there may be unintended consequences of bariatric surgery that are related to long term adverse skeletal effects and nutritional deficiencies.  
1/3/201820 minutes, 17 seconds
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New Therapies for Treating Hepatitis C Virus: Impact on Laboratory Testing Recommendations and Clinical Management

Hepatitis C virus or HCV infection affects close to 150 million people worldwide.  In the U.S., it is estimated that as many as five million people have been infected with the Hepatitis C virus, many of whom are unaware of their infection.  HCV is now the most common indication for liver transplantation, and it accounts for more deaths each year than all other reportable infectious diseases combined including HIV. Advances in testing methods and antiviral therapies with increased development of infrastructures that improve access care now make eradication of HCV infection in well-resourced countries a realistic goal.  However, challenges in implementing screening programs and continues evolution of assay methods and screening protocols raise several issues for how clinicians and laboratories respond to the changing landscape for HCV diagnosis and treatment.  The December 2017 issue of Clinical Chemistry includes a Q&A article with several experts who offer their insights and opinions on this topic.
12/20/20177 minutes, 15 seconds
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High-Resolution, Accurate-Mass (HRAM) Mass Spectrometry Urine Steroid Profiling in the Diagnosis of Adrenal Disorders

Adrenal steroid analysis plays an important role in the diagnosis of Cushing's syndrome, disorders of steroidogenesis, and adrenal tumors.  The analytical sensitivity and specificity for steroid assays have evolved over time from individual immunoassays to multiplexed mass spectrometry-based methods.  This transition has highlighted a need for simultaneous measurement of multiple steroids and their various metabolites to achieve the best diagnostic accuracy.  Therefore, metabolomic profiling performed by liquid chromatography, high-resolution accurate-mass mass spectrometry might be poised to make a substantial impact on endocrine laboratory testing.  The December 2017 Issue of Clinical Chemistry describes such a method used to determine sex- and age-based reference intervals and to perform a limited assay evaluation in patients with different adrenal diseases.
12/20/201717 minutes, 2 seconds
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January 2018 Summary Ahmad

12/20/20171 minute, 8 seconds
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January 2018 Summary Varbo

12/20/201751 seconds
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January 2018 Summary Castillo

12/20/201759 seconds
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January 2018 Summary Ndumele

12/20/201751 seconds
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January 2018 Summary Astley

12/20/201747 seconds
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January 2018 Summary Mendelian Group

12/20/201758 seconds
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Audio Summary January 2018

12/20/20176 minutes, 35 seconds
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Clinical Case Study: Unusual Cause of Abdominal Pain and Anemia

Exposure to lead in adults is typically via occupational or environmental exposures. The incidence of lead poisoning have declined worldwide due to improved industrial safety measures and the limited use of lead in paint, pipes, and canned goods. Though an increasing number of lead intoxications have been caused by the use of contaminated illicit drugs, including opium, marijuana and amphetamine, this may be due to impurities introduced during manufacturing or from deliberate addition to increase the weight for a higher selling price. A Clinical Case Study in the December 2017 issue of Clinical Chemistry discusses a series of adult patients who presented with similar symptoms and were discovered to have extremely elevated blood lead concentrations.
12/19/20179 minutes, 33 seconds
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Using Biomarkers to Guide Heart Failure Therapy

12/8/201714 minutes, 51 seconds
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Audio Summary December 2017

11/22/20177 minutes, 57 seconds
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December 2017 Summary Silbernagel

11/20/201758 seconds
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December 2017 Summary Jagodzinski

11/20/20171 minute, 8 seconds
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December 2017 Summary Tolbus

11/20/20171 minute, 9 seconds
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December 2017 Summary McCudden

11/20/201739 seconds
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December 2017 Summary Durant

11/20/201754 seconds
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December 2017 Summary Taylor

11/20/20171 minute, 5 seconds
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December 2017 Summary Hines

11/20/201757 seconds
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The Role of Procalcitonin in Diagnosis of Sepsis and Antibiotic Stewardship: Opportunities and Challenges

Sepsis is a life-threatening condition associated with significant mortality and healthcare cost.  There is increasing evidence supporting the use procalcitonin as a biomarker for diagnosis of bacterial sepsis and as a guide to discontinue antibiotic therapy.  However, concerns about the efficacy, safety, and availability of procalcitonin exist.  A Q&A article in the September 2017 issue of Clinical Chemistry asked experts with different roles in this field to share their thoughts on the challenges of procalcitonin-guided diagnosis and antibiotic therapy. 
10/26/201713 minutes, 38 seconds
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Audio Summary November 2017

10/24/20178 minutes, 3 seconds
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November 2017 Summary Diao

10/24/201759 seconds
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November 2017 Summary Costa

10/24/201748 seconds
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November 2017 Summary Noceti

10/24/20171 minute, 12 seconds
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November 2017 Summary Ferencik

10/24/20171 minute, 1 second
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November 2017 Summary Langsted

10/24/20171 minute
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November 2017 Summary Cao

10/24/201752 seconds
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November 2017 Summary Pan

10/24/201754 seconds
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High-Sensitivity Troponin: Star Player but No Lone Hero

Richard Body
10/13/20179 minutes, 43 seconds
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Implementing a Reference Measurement System for C-Peptide: Successes and Lessons Learned

Randie Little
10/4/20177 minutes, 41 seconds
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Audio Summary October 2017

9/22/20177 minutes, 31 seconds
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October 2017 Summary DeGrande

9/22/201751 seconds
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October 2017 Summary Gool

9/22/201754 seconds
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October 2017 Summary Pryor

9/22/201741 seconds
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October 2017 Summary Lam

9/22/201753 seconds
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October 2017 Summary Ladas

9/22/201744 seconds
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October 2017 Summary Sandoval

9/22/20171 minute, 9 seconds
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October 2017 Summary Bredemeier

9/22/20171 minute, 4 seconds
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Overinterpretation of Research Findings: Evidence of “Spin” in Systematic Reviews of Diagnostic Accuracy Studies

Matthew McInnes
8/23/201710 minutes, 2 seconds
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Patient Privacy and Clinical Laboratory Data

Jason Park
8/23/201711 minutes, 34 seconds
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When Gender Identity Doesn't Equal Sex Recorded at Birth: The Role of the Laboratory in Providing Effective Healthcare to the Transgender Community

Dina Greene
8/23/20177 minutes, 51 seconds
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Audio Summary September2017

8/18/20178 minutes, 57 seconds
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September 2017 Summary Carobene

8/18/20171 minute, 10 seconds
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September 2017 Summary Cardenas-Gonzalez

8/18/20171 minute, 6 seconds
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September 2017 Summary Do

8/18/20171 minute, 7 seconds
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September 2017 Summary Ebai

8/18/201755 seconds
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September 2017 Summary Kahaly

8/18/20171 minute, 2 seconds
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September 2017 Audio Summary Kahraman

8/18/20171 minute, 7 seconds
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September 2017 Audio Summary Zhang

8/18/20171 minute, 10 seconds
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Facilitating Prospective Registration of Diagnostic Accuracy Studies: A STARD Initiative

Daniel Korevaar
8/17/20177 minutes, 16 seconds
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August 2017 Audio Summary

7/25/20177 minutes, 10 seconds
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August 2017 Audio Summary Eggers

7/24/201750 seconds
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August 2017 Audio Summary Wang

7/24/201758 seconds
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August 2017 Audio Summary Sillence

7/24/201752 seconds
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August 2017 Audio Summary Bietenbeck

7/24/20171 minute, 3 seconds
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August 2017 Audio Summary Christenson

7/24/201740 seconds
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August 2017 Audio Summary Liao

7/24/201743 seconds
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August 2017 Audio Summary McGrath

7/24/201741 seconds
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Harmonization: Its Time Has Come

Greg Miller
7/17/201712 minutes, 44 seconds
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Temporal Biomarker Profiling Reveals Longitudinal Changes in Risk of Death or Myocardial Infarction in Non–ST-Segment Elevation Acute Coronary Syndrome

Mark Chan
7/6/201716 minutes, 38 seconds
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July 2017 Audio Summary

6/22/20179 minutes, 29 seconds
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July 2017 Audio Summary Schrock

6/22/201757 seconds
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July 2017 Audio Summary Harada

6/22/201754 seconds
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July 2017 Audio Summary Liao

6/22/201741 seconds
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July 2017 Audio Summary Ong

6/22/20171 minute
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July 2017 Audio Summary Thienpont

6/22/201755 seconds
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July 2017 Audio Summary Duprez

6/22/20171 minute, 17 seconds
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July 2017 Audio Summary Wildi

6/22/20171 minute, 18 seconds
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July 2017 Audio Summary Chan

6/22/20171 minute, 7 seconds
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Thrombin-Mediated Degradation of Human Cardiac Troponin T

6/15/20177 minutes, 32 seconds
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Distinguishing Intake of New Synthetic Cannabinoids ADB-PINACA and 5F-ADB-PINACA with Human Hepatocyte Metabolites and High-Resolution Mass Spectrometry

5/24/201717 minutes, 23 seconds
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June 2017 Audio Summary

5/24/20178 minutes, 16 seconds
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June 2017 Audio Summary Kobylecki

5/24/201752 seconds
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June 2017 Audio Summary Carobene

5/24/201757 seconds
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June 2017 Audio Summary Lian

5/24/20171 minute, 18 seconds
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June 2017 Audio Summary Liu

5/24/201753 seconds
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June 2017 Audio Summary Lewis

5/24/201757 seconds
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June 2017 Audio Summary Dieplinger

5/24/20171 minute, 9 seconds
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June 2017 Audio Summary Katrukha

5/24/201752 seconds
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Fifty Shades of Yellow

5/18/201713 minutes, 14 seconds
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May 2017 Audio Summary

4/24/20177 minutes, 48 seconds
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Association of Blood Eosinophil and Blood Neutrophil Counts with Asthma Exacerbations in the Copenhagen General Population Study

 Asthma is a chronic inflammatory lung disease that affects an estimated 300 million people worldwide, including 25 million in the United States. There are currently no definitive diagnostic tests for asthma. Diagnosis is based on history and tests of respiratory function.
4/11/20175 minutes, 41 seconds
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FullIssueSummary April2017

4/1/201711 minutes, 36 seconds
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Molecular Portrait of Metastasis-Competent Circulating Tumor Cells in Colon Cancer Reveals the Crucial Role of Genes Regulating Energy Metabolism and DNA Repair

Circulating tumor cells are fundamental to the concept of a liquid biopsy for cancer.  These cells are shed from the primary tumor and are carried in the blood to remote sites, potentially leading to metastasis formation.  Understanding more about this process is critical as tumor metastasis is a leading cause of cancer death.  Yet this is challenging as the number of circulating tumor cells in an individual is very small, making them difficult to study in detail. Cell lines derived from circulating tumor cells can be created to overcome this problem.  This has been done in colon cancer, for example, and enables researchers to examine molecular and functional differences between the circulating tumor cells and those from the primary tumor.  In this way, researchers may further our ability to identify and characterize cells that initiate metastasis and to ultimately develop new therapies to stop them. An original report in the January 2017 issue of Clinical Chemistry describes the differential gene expression between colon cancer cell lines derived from circulating tumor cells and the primary tumor.  Dr. Catherine Alix-Panabières is the primary author of this article and joins us for this podcast.
3/27/201710 minutes, 32 seconds
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Are THC Levels in Oral Fluids and Blood Plasma Comparable after Oral Ingestion of Edibles Containing Cannabis or THC?

Cannabis and its products are the most widely used illicit drugs world-wide, with the majority of countries currently harboring consumers and producers. Medicalization and legalization of the drug...
3/13/201722 minutes, 5 seconds
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High Glycated Albumin and Mortality in Persons with Diabetes Mellitus on Hemodialysis

Monitoring of glycemic control with hemoglobin A1c (A1c) in hemodialysis patients may be compromised by anemia and erythropoietin therapy.
3/1/20177 minutes, 50 seconds
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Bone Turnover Markers in the Diagnosis and Monitoring

Disorders of bone metabolism, most notably osteoporosis, are highly prevalent and predispose to fractures, causing high patient morbidity and mortality.
3/1/201712 minutes, 21 seconds
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Clinical Chemistry's Commitment to Education: Stronger than Ever

In addition to focusing on the publication of cutting-edge scientific reports, Clinical Chemistry has shown a genuine commitment to education by devoting a substantial portion of its pages to educational articles since 2008.
2/17/20177 minutes, 30 seconds
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Patient Consent and the Commercialization of Lab Data

Every year, commercial testing labs earn millions of dollars from selling the anonymized results of blood, urine, tissue, and other tests with almost no public scrutiny or debate.2 Few outside the industry know anything about this trade, and lab workers are often in the dark as well.
2/17/201711 minutes, 31 seconds
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FullIssueSummary February2017

2/1/201714 minutes, 45 seconds
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Universal Haplotype-Based Noninvasive Prenatal Testing for Single Gene Diseases

Researchers have developed approaches for the noninvasive prenatal testing of single gene diseases.
1/26/201710 minutes, 35 seconds
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Diurnal Rhythm of Cardiac Troponin: Consequences for the Diagnosis of Acute Myocardial Infarction

Interpretation of serial high-sensitivity cardiac troponin (hs-cTn) measurements for the diagnosis of acute myocardial infarction (AMI) assumes random fluctuation of hs-cTn around an individual's homeostatic set point.
1/26/20179 minutes, 7 seconds
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Short-term variability of vitamin D-related biomarkers

Quantifying the variability of biomarkers is important, as high within-person variability can lead to misclassification of individuals. Short-term variability of important markers of vitamin D metabolism is relatively unknown.
1/17/20174 minutes, 12 seconds
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Interpreting Cardiac Biomarkers in the Setting of Chronic Kidney Disease

Chronic kidney disease (CKD) is common, particularly in those of advanced age.
1/5/20179 minutes, 55 seconds
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Myocardial Infarction Type 2 and Myocardial Injury

The development and implementation of sensitive and high-sensitivity cardiac troponin assays has not only expedited the early ruling in and ruling out of acute myocardial infarction, but has also contributed to the identification of patients at risk for myocardial injury with necrosis, as confirmed by the presence of cardiac troponin concentrations above the 99th percentile.
1/5/201719 minutes, 59 seconds
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Biomarkers for Clinical Decision-Making in the Management of Pulmonary Embolism

Pulmonary embolism (PE) is associated with high all-cause and PE-related mortality and requires individualized management.
1/5/20177 minutes, 44 seconds
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Immediate Rule-Out of Acute Myocardial Infarction Using Electrocardiogram and Baseline High-Sensitivity Troponin I

Serial measurements of high-sensitivity troponin are used to rule out acute myocardial infarction (AMI) with an assay specific cutoff at the 99th percentile.
1/5/20174 minutes, 48 seconds
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Trimethylamine N-oxide and Risk Stratification after Acute Myocardial Infarction

Risk stratification in acute myocardial infarction (MI) remains a clinical challenge.
1/5/20175 minutes, 41 seconds
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Early rule-out and rule-in strategies for myocardial infarction

Patients with chest pain comprise a large proportion of emergency presentations and place a major burden on healthcare resources.
1/5/201715 minutes, 32 seconds
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FullIssueSummary January2017

1/1/201720 minutes, 12 seconds
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Alcohol consumption and cardiac biomarkers: The Atherosclerosis Risk in Communities (ARIC) Study

The role of alcohol in the development of subclinical cardiovascular disease is unclear. We examined the association between alcohol consumption and markers of subclinical cardiac damage and wall stress.
11/7/201612 minutes, 32 seconds
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Is the $1,000 genome as near as we think? A cost analysis of next-generation sequencing

The substantial technological advancements in next-generation sequencing (NGS), combined with dropping costs, have allowed for a swift diffusion of NGS applications in clinical settings.
10/31/201612 minutes, 6 seconds
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Optimization of a Moving Averages Program Using a Simulated Annealing Algorithm

This is the November2016 issue of Clinical Chemistry, Volume 62, Issue 11.
10/28/20168 minutes, 50 seconds
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A Nanoparticle-Lectin Immunoassay Improves Discrimination of Serum CA125 from Malignant and Benign Sources

Measurement of serum cancer antigen 125 (CA125) is the standard approach for epithelial ovarian cancer (EOC) diagnostics and follow-up. However, the clinical specificity is not optimal because increased values are also detected in healthy controls and in benign diseases.
10/18/20169 minutes, 33 seconds
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Comprehensive Assessment of M-Proteins Using Nanobody Enrichment Coupled to MALDI-TOF Mass Spectrometry

Electrophoretic separation of serum and urine proteins has played a central role in diagnosing and monitoring plasma cell disorders. Despite limitations in resolution and analytical sensitivity, plus the necessity for adjunct methods, protein gel electrophoresis and immunofixation electrophoresis (IFE) remain front-line tests.
10/12/20165 minutes, 43 seconds
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A Study of Hypermethylated Circulating Tumor

Circulating tumor DNA (ctDNA) has emerged as a good candidate for tracking tumor dynamics in different cancer types, potentially avoiding repeated tumor biopsies. Many different genes can be mutated within a tumor, complicating procedures for tumor monitoring, even with highly sensitive next-generation sequencing (NGS) strategies.
9/26/20168 minutes, 42 seconds
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What Can a Clinical Chemist Learn from Aviation?

Most people perceive pilots who don't fly for the commercial airlines as daredevils who always "fall out of the sky." My acquaintances are genuinely surprised to learn that I, a medical doctor and a clinical chemist, am one of those presumed reckless individuals.
9/19/201619 minutes, 27 seconds
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FullIssueSummary September16

9/1/20169 minutes, 23 seconds
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Full Audio Summary September16

9/1/20169 minutes, 23 seconds
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Discordance with 3 Cardiac Troponin I and T Assays: Implications for the 99th Percentile Cutoff

Cardiac troponin is integral in the investigation of acute coronary syndromes. Modern high-sensitivity versions of troponin assays are able to detect very low concentrations and potentially identify disease sooner.
8/29/20167 minutes, 53 seconds
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Can't Touch This! Contamination of Laboratory Equipment with Bloodborne Pathogens

The Ebola outbreak in the fall of 2015 led to emergent responses by hospitals to prepare for potential patients. This included preparedness efforts by laboratories, which was challenging as they was evolving and at times contradicting information about how samples from patients under investigation for Ebola should be handled.
8/29/201614 minutes, 33 seconds
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The Promise-and Pitfalls-of Computerized Provider Alerts for Laboratory Test Ordering

It is no secret that laboratory tests are overordered in the clinical environment; a recent metaanalysis demonstrated that tests are overused by 20.6% on average. The ordering of unnecessary tests on such a massive scale may impact not only direct healthcare costs, but also patient safety, and may lead to unnecessary follow-up investigation and iatrogenic blood loss due to excess phlebotomy. Unfortunately, in large hospitals with high patient volumes, it would be impractical for laboratory directors to evaluate every test individually for appropriateness, and even more difficult to convince providers to change ordering practices. Meeting other evidence-based quality objectives in laboratory ordering is similarly labor-intensive.
8/15/201618 minutes, 21 seconds
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Ready, Set, Type! Proteomics vs Agglutination for Escherichia coli H Antigen Confirmation

Initial detection and reporting by clinical microbiology laboratories is a sentinel marker for foodborne outbreak surveillance systems. Initiation of a public health investigation is reliant on the rapid initial identification of pathogens of interest. Diagnostics for Escherichia coli have evolved to reduce identification turnaround time, incorporating technologies for rapid identification (MALDI-TOF MS) and serogrouping (O157 antiserum or latex agglutination). Reporting of these isolates to a public health agency may initiate further laboratory investigations, such as pulsed-field gel electrophoresis, for confirmation that isolates may be related to a common source (clonal population).
8/15/201617 minutes, 56 seconds
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Classifying Germline Sequence Variants in the Era of Next-Generation Sequencing

Next generation sequencing is increasingly available in clinical laboratories, enabling the sequencing of more genes for relatively lower costs.
7/18/201616 minutes, 6 seconds
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A 30-Year-Old Patient Who Refuses to Be Drug Tested

The practice of medicine is not always clear-cut. Healthcare providersare often faced with difficult decisions such as what test to perform on patients, what treatment should be given, and what type of follow-up is needed. Providers have a duty to enhance the wellbeing and minimize harm to their patients.
7/11/201617 minutes, 16 seconds
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Full Audio Summary July16

7/1/201652 seconds
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Plasma Branched-Chain Amino Acids and Incident Cardiovascular Disease in the PREDIMED Trial

The role of branched-chain amino acids (BCAAs) in cardiovascular disease (CVD) remains poorly understood. We hypothesized that baseline BCAA concentrations predict future risk of CVD and that a Mediterranean diet (MedDiet) intervention may counteract this effect.
6/30/201610 minutes, 54 seconds
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Incorporating Clinical Considerations into Statistical Analyses of Markers: A Quiet Revolution in How We Think About Data

The basic problem shared by many of the biostatistical methods used in marker research is that they relate numbers to other numbers, rather than to anything that we might care about in the real world.
6/30/201611 minutes, 36 seconds
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Smartphones Can Monitor Medical Center Pneumatic Tube Systems

Pneumatic tube systems have become a primary way of automating the transport of clinical specimens in medical centers. These may travel within the floors of a building and also between buildings. Although this provides convenience and speed of transport, the excessive acceleration forces and the prolonged time and distance travelled have been linked to pre-analytical variation including hemolysis of blood specimens. As a result, it has been suggested that users regularly assess the forces encountered in a pneumatic tube system.
6/27/20167 minutes, 12 seconds
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Postmarket Surveillance of Point-of-Care Glucose Meters through Analysis of Electronic Medical Records

Postmarket surveillance is an important monitor of the safety of pharmaceuticals and medical devices after regulatory approval and entry to the market. Although premarket studies are substantial, costs prohibit examination of heterogeneous populations or rare events over long durations.
6/21/201624 minutes, 48 seconds
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Pharmacists in the Laboratory Space: Friends or Foes?

There are nearly 60,000 community pharmacies in the United States today. In addition to being sources of prescriptions and medications, they're also often a site for wellness visits, vaccinations, acute illness diagnosis and treatment, as well as monitoring of chronic disease. Pharmacies often offer a convenient alternative to physician offices because of their accessible locations and extended service hours. Approximately 18% of pharmacies in the U.S. have CLIA-waived status, which means they can perform any of the approximately 120 CLIA-waived tests. Many pharmacists help patients make drug dosage decisions in specialized centers such as coumadin in diabetes clinics. With pharmacists performing and interpreting more laboratory tests, what does this mean for the clinical laboratory community? Do pharmacists receive the proper training to perform and interpret laboratory tests? And how do we ensure the quality of testing?
6/13/201615 minutes
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FullIssueSummary June2016

6/1/20167 minutes, 37 seconds
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Different Susceptibility of B-Type Natriuretic Peptide (BNP) and BNP Precursor (proBNP) to Cleavage by Neprilysin: The N-Terminal Part Does Matter

Protease neprilysin is known to be responsible for the degradation of natriuretic peptides. A recent heart failure (HF) drug, LCZ696 (EntrestoTM), that combines a neprilysin inhibitor and an angiotensin II receptor inhibitor was suggested to augment circulating B-type natriuretic peptide (BNP) concentrations, making the results of BNP measurements diagnostically ambiguous. Because the main form of measured BNP in HF patients is represented by its uncleaved precursor, proBNP, it is important to know the susceptibility of proBNP to cleavage by neprilysin.
5/31/20169 minutes, 57 seconds
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Soluble CRTC3: A Newly Identified Protein Released by Adipose Tissue That Is Associated with Childhood Obesity

CREB-regulated transcription coactivator 3 (CRTC3) is found in adipocytes, where it may promote obesity through disruption of catecholamine signaling. We wished to assess whether CRTC3 is a soluble protein secreted by adipose tissue, explore whether CRTC3 is detectable and quantifiable in the circulation, and ascertain whether CRTC3 serum concentrations are related to metabolic markers in children.
5/23/20169 minutes, 27 seconds
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Effect of Dried Blood Spot Quality on Newborn Screening Analyte Concentrations and Recommendations for Minimum Acceptance Criteria for Sample Analysis

The analysis of dried blood spots has been used routinely for newborn screening since the early 1970s, and the number of disorders screened has expanded substantially in recent years. However, there is a lack of evidence regarding minimum blood spot quality acceptance criteria for sample analysis.
5/3/201610 minutes, 8 seconds
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FullIssueSummary May2016

5/1/20167 minutes, 36 seconds
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Nonfasting Sample for the Determination of Routine Lipid Profile: Is It an Idea Whose Time Has Come?

For many years the determination of a routine lipid profile (total, LDL, and HDL cholesterol and triglycerides) has been done routinely in the clinical laboratory using a blood specimen that is collected in the fasting state.
4/26/201613 minutes, 5 seconds
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Mass Spectrometry–Based Adrenal and Peripheral Venous Steroid

Primary aldosteronism,also known as Conn's syndrome,is a form of endocrine hypertension that results from excess product of aldosterone by the adrenal glands.
4/19/201614 minutes, 43 seconds
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Paper-Based Quantification of Male Fertility Potential

More than 70 million couples worldwide are affected by infertility, with male-factor infertility accounting for about half of the cases. Semen analysis is critical for determining male fertility potential, but conventional testing is costly and complex. Here, we demonstrate a paper-based microfluidic approach to quantify male fertility potential, simultaneously measuring 3 critical semen parameters in 10 min: live and motile sperm concentrations and sperm motility.
4/5/201610 minutes, 20 seconds
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FullIssueSummary April2016

4/1/20169 minutes, 5 seconds
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Clostridium difficile—Diagnostic and Clinical Challenges

Clostridium difficile is the most common cause of healthcare associated infections in the United States and is increasingly recognized as a pathogen in the community. This organism is considered to be one of the most urgent antibiotic resistant threats to public health, and can cause a variety of clinical manifestations ranging from asymptomatic to mild diarrhea, to toxic megacolon, and even death.
3/15/201618 minutes, 17 seconds
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Paper-Based Quantification of Male Fertility Potential

More than 70 million couples worldwide are affected by infertility, with male-factor infertility accounting for about half of the cases. Semen analysis is critical for determining male fertility potential, but conventional testing is costly and complex. Here, we demonstrate a paper-based microfluidic approach to quantify male fertility potential, simultaneously measuring 3 critical semen parameters in 10 min: live and motile sperm concentrations and sperm motility.
3/5/20166 minutes, 14 seconds
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FullIssueSummary March2016

3/1/20169 minutes, 34 seconds
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Impact of Implementing Preanalytical Laboratory Standards on the Diagnosis of Gestational Diabetes Mellitus: A Prospective Observational Study

Gestational diabetes mellitus (GDM) is associated with adverse pregnancy outcomes, but risk is reduced with identification and early treatment. Glucose measurements are affected by preanalytical sample handling, such as temperature of storage, phlebotomy–analysis interval, and use of a glycolysis inhibitor. We evaluated glucose concentrations and the incidence of GDM after strict implementation of the American Diabetes Association (ADA) preanalytical guidelines, compared with usual hospital conditions.
2/22/20167 minutes, 14 seconds
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Effect of Vitamin D or Activated Vitamin D on Circulating 1,25- Dihydroxyvitamin D Concentrations

Evidence is accumulating that circulating 1,25-dihydroxyvitamin D [1,25(OH)2D] concentrations are inversely related to overall mortality.
2/16/20168 minutes, 13 seconds
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FullIssueSummary February2016

2/1/20168 minutes, 58 seconds
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Serum Total Testosterone Concentrations in the US Household Population from the NHANES 2011–2012 Study Population

Limited information is available about testosterone concentrations representative of the general US population, especially children, women, and non-Hispanic Asians.
2/1/20169 minutes, 54 seconds
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US Food and Drug Administration Perspectives on Clinical Mass Spectrometry

Mass spectrometry–based in vitro diagnostic devices that measure proteins and peptides are underutilized in clinical practice, and none has been cleared or approved by the Food and Drug Administration (FDA) for marketing or for use in clinical trials. One way to increase their utilization is through enhanced interactions between the FDA and the clinical mass spectrometry community to improve the validation and regulatory review of these devices.
1/27/20168 minutes, 52 seconds
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Measurement by a Novel LC-MS/MS Methodology Reveals Similar Serum Concentrations of Vitamin D–Binding Protein in Blacks and Whites

There are now numerous epidemiological studies that have demonstrated that low plasma concentrations of vitamin D are associated with not only bone related disorders, but with a wide variety of adverse health outcomes.
1/27/201621 minutes, 50 seconds
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Ambient Ionization Mass Spectrometry for Point-of-Care Diagnostics and Other Clinical Measurements

A driving force in the development of point-of-care diagnostics is to conveniently provide information without delay so that healthcare decisions can be made while the patient is on-site. Most point-of-care devices are based on immunochemical, electrochemicalor colorimetric techniques that can be miniaturized and made portable.
1/8/201621 minutes, 36 seconds
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Mass Spectrometry Selectivity, Specifically

A mass spectrometer is a sensitive and specific instrument." In some form or another, this is often the label applied to the platform by all manner of professions (chemists, physicists, biologists, physicians, clinicians, and laboratorians, novice and expert alike). This is a view of mass spectrometry (MS) through rose-colored glasses. It should be properly said that MS can be sensitive and specific, but not without due consideration for the measurement to be performed. In clinical laboratories, this measurement is the extraction, detection, and often, quantification of a compound of interest from a biological matrix.
1/4/201610 minutes, 5 seconds
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High-Resolution Mass Spectrometry for Characterizing the Metabolism of Synthetic Cannabinoid THJ-018 and Its 5-Fluoro Analog THJ-2201 after Incubation in Human Hepatocytes

Despite increasing prevalence of novel psychoactive substances, no human metabolism data are currently available, complicating laboratory documentation of intake in urine samples and assessment of the drugs' pharmacodynamic, pharmacokinetic, and toxicological properties. In 2014, THJ-018 and THJ-2201, synthetic cannabinoid indazole analogs of JWH-018 and AM-2201, were identified, with the National Forensic Laboratory Information System containing 220 THJ-2201 reports. Because of numerous adverse events, the Drug Enforcement Administration listed THJ-2201 as Schedule I in January 2015.
1/4/201619 minutes, 49 seconds
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Protein-Specific Glycoprofiling for Patient Diagnostics

Protein glycosylation is increasingly recognized as a crucial modulator of protein function, offering a third layer of biological information over genomics and proteomics. Modern tools for analyzing released N-glycans from cells and body fluids, i.e., the glycome, have shown abnormal protein glycosylation in numerous human diseases. These include both genetic and acquired diseases, ranging from diabetes, cancer, and inflammatory disease to neurodegenerative and neuromuscular disease. Insights from this novel field in human medicine provide exciting perspectives toward understanding disease processes, identifying therapeutic targets, and designing individualized diagnostics based on protein concentrations and glycosylation status. However, the main question is how we can translate this information into concrete biomarkers in a clinical diagnostic setting, with high demands on technical robustness and the ability to interpret results within specific patient groups.
1/4/20167 minutes, 16 seconds
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Clonotypic Light Chain Peptides Identified for Monitoring Minimal Residual Disease in Multiple Myeloma without Bone Marrow Aspiration

Analytically sensitive techniques for measuring minimal residual disease (MRD) in multiple myeloma (MM) currently require invasive and costly bone marrow aspiration. These methods include immunohistochemistry (IHC), flow cytometry, quantitative PCR, and next-generation sequencing. An ideal MM MRD test would be a serum-based test sensitive enough to detect low concentrations of Ig secreted from multifocal lesions.
1/4/201611 minutes, 39 seconds
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FullIssueSummary January2016

1/1/201615 minutes, 33 seconds
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Performance of Cystatin C– and Creatinine-Based Estimated Glomerular Filtration Rate Equations Depends on Patient Characteristics

The Kidney Disease Improving Global Outcomes (KDIGO) guideline recommends use of a cystatin C–based estimated glomerular filtration rate (eGFR) to confirm creatinine-based eGFR between 45 and 59 mL · min−1 · (1.73 m2)−1. Prior studies have demonstrated that comorbidities such as solid-organ transplant strongly influence the relationship between measured GFR, creatinine, and cystatin C. Our objective was to evaluate the performance of cystatin C–based eGFR equations compared with creatinine-based eGFR and measured GFR across different clinical presentations.
12/29/20158 minutes, 26 seconds
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"Big Data" in Laboratory Medicine

Informatics is changing the processes behind laboratory medicine. With ever-growing demands on laboratory medicine professionals not only to collect and interpret omics data in the era of the Precision Medicine Initiative, but also to ensure high-quality, low-cost patient management in the structure of accountable care organizations, we have invited several experts to discuss their take on "big data."
12/21/201533 minutes, 17 seconds
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Optimizing Early Rule-Out Strategies for Acute Myocardial Infarction: Utility of 1-Hour Copeptin

Combined testing of high-sensitivity cardiac troponin T (hs-cTnT) and copeptin at presentation provides a very high—although still imperfect—negative predictive value (NPV) for the early rule-out of acute myocardial infarction (AMI). We hypothesized that a second copeptin measurement at 1 h might further increase the NPV.
12/8/201510 minutes, 48 seconds
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Obese Nondiabetic Pregnancies and High Maternal Glycated Hemoglobin at Delivery as an Indicator of Offspring and Maternal Postpartum Risks:

We investigated whether obese pregnant women negative for gestational diabetes (GDM) still experience dysglycemia, as indicated by high glycated hemoglobin (Hb A1c) at delivery, and whether this impacts offspring and long-term maternal outcomes.
11/17/20157 minutes, 22 seconds
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STARD 2015: An updated list of essential items for reporting diagnostic accuracy studies

STARD 2015: An updated list of essential items for reporting diagnostic accuracy studies
11/4/20159 minutes, 42 seconds
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FullIssueSummary November2015

11/1/20157 minutes, 40 seconds
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Extreme Nonfasting Remnant Cholesterol vs Extreme LDL Cholesterol as Contributors to Cardiovascular Disease and All-Cause Mortality in 90000 Individuals from the General Population

Increased concentrations of non-fasting remnant cholesterol are thought to be atherogenic in thesame way as LDL cholesterol,by transport into andaccumulationof lipid into the arterial wall.
10/26/201510 minutes, 29 seconds
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Clopidogrel and CYP2C19: Pharmacogenetic Testing Ready for Clinical Prime Time?

For patients with acute coronary syndrome who have undergone interventions such as placement of a stent, it has become standard practice to treat them with a combination of aspirin and opidogrel to prevent subsequent thrombotic events; however, clopidogrel is a drug that requires activation by a liver enzyme, CYP2C19.
10/8/201511 minutes, 11 seconds
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Nonfasting Lipid Profiles: The Way of the Future

Most of us are familiar with the link between elevated concentrations of lipids in our blood and an increased risk for cardiovascular disease which is responsible for about 500,000 deaths each year in the United States. It has been common practice to measure those lipids only after a period of fasting. This practice was put in place because of the known increases in triglycerides that can occur after eating; however, fasting lipid profiles have not actually been shown to be superior to nonfasting lipid profiles in assessing the risk for cardiovascular disease.
10/8/20156 minutes, 30 seconds
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Next-Generation Microfluidic Point-of-Care

Microfluidics has attracted a lot of attention across a wide range of disciplines because of the potential for using it to analyze tiny samples and to integrate processes onto handheld Labs-on-a-Chip. So instead of sending vials of blood to a central laboratory and getting results back days later, you can imagine a Microfluidic device that can give you the same performance, but with a finger prick of blood right on the spot, in minutes.
10/1/20156 minutes, 22 seconds
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FullIssueSummary October2015

10/1/20156 minutes, 56 seconds
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Difference in Bias Approach for Commutability Assessment: Application to Frozen Pools of Human Serum Measured by 8 Direct Methods for HDL and LDL Cholesterol.

Commutability is an important concept in determining traceability and relationship among different methods used to measure the same analyte, but how to delineate commutable materials is not straightforward and a number of techniques have been proposed.
9/8/201510 minutes, 49 seconds
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FullIssueSummary September2015

9/1/20157 minutes, 10 seconds
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Pediatric Lipid Screening Rates in the US Are Low: What Can Labs Do to Help?

Several studies have suggested that prevention of atherosclerosis should begin in childhood as dyslipidemia in children is associated with severity of atherosclerotic lesions later in life. Pediatric lipid screening aims to identify children and adolescents with dyslipidemia, including both those with more severe genetic cases and those cases with mild to moderate lipid elevation due to secondary causes.
8/24/20158 minutes, 54 seconds
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FullIssueSummary August2015

8/1/20157 minutes, 22 seconds
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Why Disruptive Innovations Matter in Laboratory Diagnostics

Advances in clinical laboratory testing have had a profound impact in improving the quality of medical care. Today laboratory diagnostic tests inform physicians and patients of the exact causes of a number of diseases and disorders, including cancer, chronic diseases, infectious diseases, sexually transmitted diseases, and genetic disorders.
7/27/201515 minutes, 50 seconds
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Intersection of DNA Privacy and Whole-Genome Sequencing

In the past decade there has been a rapid increase in the number of individuals who have had their entire genome sequenced. Over the next decade that number will go from the thousands to the millions. These people are having their genomes sequenced for research, recreational interest, and clinical testing.
7/14/20159 minutes, 40 seconds
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FullIssueSummary July2015

7/1/20157 minutes, 35 seconds
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24,25-Dihydroxyvitamin D3 and Vitamin D Status of Community-Dwelling Black and White Americans

Vitamin D insufficiency has been widely associated with numerous negative health outcomes, including higher all-cause mortality, although no mechanism has been formally established for such observations.
6/29/201512 minutes, 13 seconds
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Preanalytical Considerations in the Design of Clinical Trials and Epidemiological Studies

Clinical trials, epidemiologic and most types of clinical research, include the acquisition of biological samples to be analyzed either immediately after collection, or in the future for biomarkers related to the study hypotheses. Tested biomarkers include those used to monitor subjects' health and to detect harmful side effects or to follow impact of therapeutic interventions.
6/23/201510 minutes, 26 seconds
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To Monitor Dabigatran or Not: A Matter of Patient Safety

Biomarkers are playing increasingly important roles in the detection and management of patients with cancer. Despite an enormous number of publications on cancer biomarkers, few of these biomarkers are in widespread clinical use.
6/15/201513 minutes, 14 seconds
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Validation of New Cancer Biomarkers: A Position Statement from the European Group on Tumor Markers

Biomarkers are playing increasingly important roles in the detection and management of patients with cancer. Despite an enormous number of publications on cancer biomarkers, few of these biomarkers are in widespread clinical use.
6/15/20157 minutes, 13 seconds
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Fasting is not routinely required for determination of a lipid profile: clinical and laboratory implications including flagging at desirable concentration cutpoints—a joint consensus statement from the European Atherosclerosis Society and European Feder

6/1/20158 minutes, 6 seconds
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FullIssueSummary June2015

6/1/20157 minutes, 13 seconds
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Investigation of 2 Models to Set and Evaluate Quality Targets for Hb A1c: Biological Variation and Sigma-Metrics

A major objective of the International Federation of Clinical Chemistry and Laboratory Medicine, or IFCC, Task Force on implementation of Hemoglobin A1c Standardization is to develop quality targets with the measurement of Hemoglobin A1c.
5/5/201510 minutes, 13 seconds
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FullIssueSummary May2015

5/1/20156 minutes, 49 seconds
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Current Evidence and Future Perspectives on the Effective Practice of Patient-Centered Laboratory Medicine

Medical laboratories provide valuable services to aid in patient diagnosis and management, but systematic evidence of laboratory medicine's specific contribution to the overall process of healthcare is not easy to come by. Even the widely quoted claim that laboratory medicine results are responsible for a 70% of all clinical decisions seems to elude objective verification.
4/20/201511 minutes, 7 seconds
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Vitamin D: The More We Know, the Less We Know

Over the past 20 years or so there have been several studies published showing an association between decreased concentrations of vitamin D in blood and the risks of cardiovascular disease, stroke, cancer, fractures, even overall mortality.
4/6/201515 minutes, 25 seconds
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FullIssueSummary April2015

4/1/20158 minutes, 12 seconds
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Qualitative Point-of-Care Human Chorionic Gonadotropin Testing: Can We Defuse This Ticking Time Bomb?

Rapid urine pregnancy tests have been available for over 30 years. They can be performed in minutes and are used at home, in doctors' offices and in emergency rooms. Doctors in particular rely on these tests to rule out pregnancy in order to prevent fetal injury or death that could be caused by certain medical procedures or drugs.
3/30/20159 minutes, 33 seconds
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Adaptive learning and medical education

Recently, the New England Journal of Medicine partnered with Area9 Learning, a physician-led pioneer in adaptive learning, to create a platform with smart technology that adapts to clinicians' learning goals and knowledge gaps to deliver the information they need to know.
3/23/201510 minutes, 30 seconds
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Validation of a Proposed Novel Equation for Estimating LDL Cholesterol

Aggressive strategies to lower Low Density Lipoprotein cholesterol in circulation are recommended for prevention of cardiovascular events. Most often laboratories report LDL-cholesterol based on an equation using measured values of total cholesterol, HDL - cholesterol and triglycerides, the so called Friedewald calculation named after the first author who published that work in Clinical Chemistry back in 1972.
3/17/20156 minutes, 12 seconds
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Is This a Critical, Panic, Alarm, Urgent, or Markedly Abnormal Result?

Medical laboratories frequently encounter clinically unexpected results that require timely clinical evaluation because they may indicate an imminent life-threatening condition or a major clinical deterioration. Laboratories, therefore, need to identify and report such results sooner than they normally would, and have policies and procedures that minimize the possibility of patient harm due to delayed clinical attention. The concept of these so-called "panic values" was raised by George Lundberg in 1970, but a variety of other terms have since appeared in the literature. For example, urgent, critical, acute, alert, abnormal, markedly or significantly abnormal, clinically significant, vital, red or orange or yellow zone values, and various combinations of terms.
3/9/20158 minutes, 41 seconds
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Proposed Regulatory Framework for Direct-to-Consumer Genetic Testing: Diagnostics vs Genetic Screening

In December 2013 the genetics laboratory 23andMe stopped marketing direct-to-consumer disease predictive genetic testing in order to comply with a directive from the US Food and Drug Administration. The FDA's action was intended to protect the American public from questionable disease risk predictions.
3/2/201510 minutes, 7 seconds
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FullIssueSummary March2015

3/1/20158 minutes, 1 second
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Learning from Our Mistakes: The Future of Validating Complex Diagnostics

Big data now seems to be everywhere. Google search queries are used to predict flu outbreaks and airline statistics or mined to determine the best hour of which day to book plane tickets. Clinical laboratories can also benefit from these trends. Given the emerging importance of reproducible data analysis pipelines, one could think of a special group of trained big data experts that could be available to regulatory agencies and to clinical laboratories for review and auditing of their processes and practices, and who may be able to offer suggestions for improvements.
2/23/201510 minutes, 58 seconds
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Association of 1,5-Anhydroglucitol with Diabetes and Microvascular Conditions

Hemoglobin A1c is widely used to monitor glycemic control and is now recommended for use in the diagnosis of diabetes. Although hemoglobin A1c has high reliability compared to the oral glucose tolerance test, there are certain settings in which such testing may be problematic and there is a growing interest in alternative markers of hyperglycemia.
2/18/20154 minutes, 33 seconds
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Moving beyond mean glycemia: 1,5-anhydroglucitol and microvascular complications of diabetes

Although hemoglobin A 1 C has high reliability , there a re certain settings in which hemoglobin A1C testing is thought to be problematic. 1 , 5 - anhydroglucitol or 1,5 - deoxyglucose is a monosaccharide originating mainly fr om foods and closely resembles glucose in structure , but is usually excreted by the kidneys.
2/9/20159 minutes, 1 second
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The Landscape of MicroRNA, Piwi-Interacting RNA, and Circular RNA in Human Saliva

Human saliva has been increasingly used for biomarker development to allow noninvasive detection of diseases. Extracellular RNA was discovered in saliva about 10 years ago, and since then the nature, origin , and characterization of salivary RNA have been actively pursued.
2/3/201512 minutes, 57 seconds
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Noninvasive Prenatal Testing for Wilson Disease by Use of Circulating Single-Molecule Amplification and Resequencing Technology (cSMART)

Prenatal testing using blood specimens rather than more invasive sampling has been successfully used for common chromosome disorders and for clinically significant copy number variations . H owever , detecting single gene disorders which are caused by mutations remains an analytical challenge.
2/3/20158 minutes, 15 seconds
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FullIssueSummary February2015

2/1/20158 minutes, 13 seconds
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Clinical Exome Performance for Reporting Secondary

In the last year, multiple clinical studies have shown the diagnosti c power of testing a patient's exome. Clinical exome testing is now available at over a dozen clinical laboratories in the US , and has been performed for thousands of patients.
1/30/201513 minutes, 43 seconds
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Next Generation Sequencing in Clinical Diagnostics: Experiences of Early

Next generation sequencing (NGS)13 technology, also known as massively parallel sequencing (MPS), is being incorporated rapidly to clinical laboratory testing. Current applications include detection of germline variants in inherited diseases, somatic variants in cancers, subpopulations of circulating cell-free DNA, and single viral or microbial genomes in infections or metamicrobial genomes in normal or altered human flora. Each application is unique and has its advantages and disadvantages.
1/26/20159 minutes, 25 seconds
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Chromosomal Instability in Cell-Free DNA Is a Serum Biomarker for Prostate Cancer

Extreme sports, extreme eating, extreme weight loss, extreme makeovers, just when you think you've heard it all, how about Extreme PCR?
1/13/201510 minutes, 1 second
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Refinement of Variant Selection for the LDL Cholesterol Genetic Risk Score in the Diagnosis of the Polygenic Form of Clinical Familial Hypercholesterolemia and Replication in Samples from 6 Countries

Familial hypercholesterolemia is a genetic disorder characterized by increased plasma concentrations of low density lipoprotein cholesterol and premature symptoms of coronary heart disease. Early identification of at-risk individuals allows changes in lifestyle including dietary intervention and drug treatment. In the January 2015 issue of Clinical Chemistry, a Special Issue devoted to Molecular Diagnostics, a group of researchers in a multi-center study describes a genetic risk score procedure that distinguishes familial hypercholesterolemia patients from healthy subjects.
1/12/201510 minutes, 56 seconds
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Extreme PCR: Efficient and Specific DNA Amplification in 15–60 Seconds

Extreme sports, extreme eating, extreme weight loss, extreme makeovers, just when you think you've heard it all, how about Extreme PCR?
1/12/201518 minutes, 20 seconds
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Total and Cause-Specific Mortality by Moderately and Markedly Increased Ferritin Concentrations: General Population Study and Metaanalysis

For decades iron has been thought of as beneficial for the body. Most everyone knows that iron deficiency is harmful and that the condition should be treated by additional iron in the diet. However, too much iron can also be harmful to the body. Ferritin is a protein that stores iron and measurement of ferritin in serum is a measure of the iron deposits in the body.
1/7/20159 minutes, 30 seconds
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FullIssueSummary January2015

1/1/201518 minutes, 9 seconds
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FullIssueSummary January2014

1/1/201513 minutes, 44 seconds
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ClinChem 201411 Vaught

Biobanking for clinical or research purposes includes the collection, processing, storage and analysis of biological specimens. It is now well-recognized that biobanking involves a complex array of technical, ethical, and regulatory considerations.
12/29/201411 minutes, 42 seconds
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Integrative Bioinformatics Analysis Reveals New Prognostic Biomarkers of Clear Cell Renal Cell Carcinoma

Kidney cancer is among the ten most frequently occurring cancers in the Western world and its incidence has been steadily rising each year. In the absence of symptoms, about 30% of patients with renalcell carcinomas are diagnosed with disease already in the metastatic stage.
12/23/20148 minutes, 57 seconds
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Clinical Genomics: When Whole Genome Sequencing is like a Whole-body CT Scan

Over the past decade genomic testing has emerged as a clinical tool available in multiple hospital and independent laboratories across the United States. In the November 2014 issue of Clinical Chemistry, an opinion article titled "Clinical Genomics: When Whole Genome Sequencing Is like a Whole-body CT Scan" framed the clinical utility of genomic testing in the context of another recently transformative test, whole-body computed tomography scanning, more commonly known as a CT scan.
12/15/201414 minutes, 35 seconds
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FullIssueSummary December2014

12/1/20148 minutes, 27 seconds
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FullIssueSummary December2015

12/1/20149 minutes, 37 seconds
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Emory: Experience with Ebola Patients

In August 2014, the first patient with the Ebola virus disease arrived in the United States and was transported to Emory Hospital in Atlanta. While hospitals and laboratories knew that it was only a matter of time before this occurred, subsequent experience has shown that not all institutions in the U.S. were equally prepared for this eventuality. In this special podcast from Clinical Chemistry, we're joined by two members of the laboratory team that treated the first healthcare workers arriving from West Africa.
11/13/20147 minutes, 33 seconds
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Microfluidic Genotyping by Rapid Serial PCR and High-Speed Melting Analysis

Turnaround times are often long in PCR-based tests because multiple reactions are usually performed in parallel using programmable thermal cyclers. These methods typically use a single protocol, placing constraints on assay design.
11/4/201410 minutes, 56 seconds
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T2 Magnetic Resonance: A Diagnostic Platform for Studying Integrated Hemostasis in Whole Blood—Proof of Concept

Thrombosis and bleeding are among the foremost causes of morbidity and mortality, and the recent introduction of novel anticoagulants, anti-thrombotic, and hemostatic drugs has increased the need for rapid and accurate assessment of their activities. While the usual laboratory assessment of hemostasis, such as prothrombin time and other coagulation tests, are often effective, these methods may not identify all bleeding disorders.
11/4/20148 minutes, 44 seconds
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Point/Counterpoint: The Quest for Clean Competition in Sports

Using prohibited substances to enhance performance in sports, often referred to as doping, is a practice that's been with us all of recorded history. In ancient time, athletes or combatants were often supplied diets and supplements considered beneficial to enhance their performance.
11/4/201421 minutes, 39 seconds
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FullIssueSummary November2014

11/1/20147 minutes, 6 seconds
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Generation of a New Cystatin C–Based Estimating Equation for Glomerular Filtration Rate by Use of 7 Assays Standardized to the International Calibrator

Estimating Glomerular Filtration Rate or GFR is important for the detection and monitoring of impairment of renal function for safety in the use of potentially nephrotoxic pharmaceuticals and radiographic contrast media, and for administration of correct dosage of drugs cleared by the kidneys. Even though it is not ideal, serum creatinine is widely used as a marker for calculating Glomerular Filtration Rates.
10/28/20148 minutes, 58 seconds
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Where Are All the New Omics-Based Tests?

Where are all the new omics-based tests? That's the question that Patrick Bossuyt asks in his paper appearing in the October 2014 issue of Clinical Chemistry. After billions of dollars worldwide have been spent upon omics-based research and announcements of many biomarker discoveries, clinical medicine has not gone through a radical change, despite all of the investment of time, money, and the collaboration of thousands of study participants.
10/21/20147 minutes, 56 seconds
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Early Detection of Fragile X Syndrome: Applications of a Novel Approach for Improved Quantitative Methylation Analysis in Venous Blood and Newborn Blood Spots.

Fragile X Syndrome is a severe neurodevelopmental disorder which is both complex and heterogeneous in both clinical phenotype and epigenotype. It is also one of the major inherited conditions co-morbid with autistic behaviors.
10/13/20149 minutes, 40 seconds
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From Lost in Translation to Paradise Found: Enabling Protein Biomarker Method Transfer by Mass Spectrometry

Fragile X Syndrome is a severe neurodevelopmental disorder which is both complex and heterogeneous in both clinical phenotype and epigenotype. It is also one of the major inherited conditions co-morbid with autistic behaviors.
10/13/201420 minutes, 46 seconds
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FullIssueSummary October2014

10/1/20147 minutes, 53 seconds
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Three of 7 Hemoglobin A1c Point-of-Care Instruments Do Not Meet Generally Accepted Analytical Performance Criteria

In a 2009 study in Clinical Chemistry, Dr. Erna Lenters and Dr. Robbert Slingerland evaluated eight different points of care instruments measuring hemoglobin A1c and came to the conclusion that six of the eight did not meet generally accepted analytical performance criteria.
9/30/201412 minutes, 59 seconds
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Influence of the Confounding Factors Age and Sex on MicroRNA Profiles from Peripheral Blood

MicroRNAs measured in blood samples are promising, minimally invasive biomarker candidates that have recently been the target of many case control studies. However, the influence of age and gender is confounding variables and MicroRNAs remain largely unknown.
9/22/20146 minutes, 13 seconds
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Maternal Plasma RNA Sequencing for Genome-Wide Transcriptomic Profiling and Identification of Pregnancy-Associated Transcripts

Analysis of circulating RNA in the plasma of pregnant women can be a powerful tool for noninvasive prenatal testing and research. However, unbiased and high throughput detection of circulating RNA in plasma is a technical challenge.
9/16/20148 minutes, 39 seconds
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Podcasts Go Platinum!

The cover of the September 2014 issue of Clinical Chemistry tells it all: one million downloads! That's the number of Clinical Chemistry podcasts that have been downloaded since the feature was initiated in 2009. It's a relatively new feature in the 60 years of publication of the journal, and the word podcast itself is only been in the vernacular for about 10 years.
9/16/20147 minutes, 5 seconds
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Measurements for 8 Common Analytes in Native Sera Identify Inadequate Standardization among 6 Routine Laboratory Assays

Performing measurements that are comparable over time and location and across assays is essential for insuring appropriate clinical and public health practice. One step toward achieving this goal is using assays that are traceable to a higher-order reference measurement system or harmonized by using internationally recognized procedures.
9/2/201418 minutes, 2 seconds
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FullIssueSummary September2014

9/1/20146 minutes, 56 seconds
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Immunoextraction - tandem mass spectrometry method for measuring intact human chorionic gonadotropin, free beta, and beta core fragment in urine

Analysis of Human Chorionic Gonadotropin or hCG is usually associated with monitoring or detecting pregnancy. It is also a protein tumor marker for some cancers. In males, hCG stimulates testosterone production and has the potential to be abused by athletes in an attempt to enhance performance in sports.
8/18/201413 minutes, 6 seconds
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To P or not to P: that is the question

There are few scientific papers that do not include the use of the P value to evaluate the statistical significance of results. However, use of this statistic may be misleading, as noted by a recent paper by Regina Nuzzo in the journal Nature. That paper served as a basis for commentary with additional examples by Drs. Jim Boyd and Tom Annesley in the July 2014 issue of Clinical Chemistry.
8/11/201410 minutes, 45 seconds
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Weekly and 90-Minute Biological Variations in Cardiac Troponin T and Cardiac Troponin I in Hemodialysis Patients and Healthy Controls

Chronic kidney disease is an important risk factor for cardiovascular morbidity and mortality. This risk is all the more important for patients undergoing hemodialysis. In the presence of clinical signs of an acute myocardial infarction, the universal definition includes the finding of a serum cardiac troponin concentration above the 99 percentile as defined by healthy individuals, together with time dependant changes in serum and troponin concentrations. But in contrast to individuals with normal kidney function the troponin concentrations in patients with chronic kidney disease, but no myocardial infarction, may already be at concentrations higher than the 99 percentile of a healthy population.
8/4/201412 minutes, 56 seconds
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FullIssueSummary August2014

8/1/20148 minutes, 26 seconds
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Biomarkers of Brain Injury in Cerebral Infections

Central nervous system infections, including diseases like meningitis and encephalitis, are important public heath concerns across the globe, as they occur commonly and/or associated with high rates of mortality and morbidity. Measurement of biomarkers provide objective indicators of normal function or pathology, and can present information that may assist diagnosis, assessment of health condition, and evaluation of treatment safety and efficacy.
7/28/201412 minutes, 43 seconds
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Novel Biomarkers for Acute Myocardial Infarction: Is MicroRNA the New Kid on the Block?

Early detection of acute myocardial infarction is crucial for deciding the course of treatment to preserve and prevent further damage to the myocardial tissue. During the last several years there has been a burgeoning interest in circulating microRNAs as potential novel biomarkers for acute myocardial infarction.
7/24/201410 minutes, 30 seconds
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ClinChem 201406 deLemos Khalili

The universal definition of myocardial infarction requires both an increase in cardiac troponin concentrations and changes in values over serial measurements. However, exact criteria regarding the magnitude of change in troponin remain elusive. In the June 2014 issue of Clinical Chemistry, two studies on biological variation of serum troponins helped shed further light on the kinetics of this important cardiac marker.!
7/14/201414 minutes, 48 seconds
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FullIssueSummary July2014

7/1/20147 minutes, 40 seconds
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Harmonization of Test Results: What Are the Challenges; How Can We Make It Better?

Laboratory test results are used to aid decisions in the diagnosis and treatment of disease. Consistent and comparable results from different measurement procedures are important for developing clinical practice guidelines, and for those guidelines to be applied to decisions about patient care. Yet results for many analytes are neither consistent nor comparable when measured using different clinical laboratory procedures.
6/30/201410 minutes, 17 seconds
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The FDA and 23andMe: Violating the First Amendment or Protecting the Rights of Consumers?

One of the largest direct consumer genetic testing facilities, 23andMe, was ordered by the US Food and Drug Administration to cease marketing its Personal Genome Service test in late 2013. This occurred after 23andMe failed to respond to questions that the FDA had about the analytical and clinical validity of this test. While some support the FDA's actions because of the test potential for harm to consumers, some advocates of the technology fault the FDA for being overly paternalistic and impeding medical advances.
6/24/201416 minutes, 56 seconds
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When a “Disease-Causing Mutation” Is Not a Pathogenic Variant

The clinical utility of molecular genetic testing relies on an accurate and comprehensive knowledge about the relationships between genes and variants, and diseases. Correctly interpreting the clinical significance of variants that may be detected continues to be a constant challenge for molecular diagnostic practice. This challenge has become substantially enlarged as next generation sequencing-based testing becomes rapidly integrated into routine clinical practice.
6/17/20149 minutes, 43 seconds
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Genetically Low Triglycerides and Mortality: Further Support for “the Earlier the Better”?

In the May issue of Clinical Chemistry, researchers involved in the Copenhagen City Heart Study found that lower nonfasting, circulating triglyceride concentrations are associated with lower all-cause mortality.
6/3/201411 minutes, 8 seconds
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FullIssueSummary June2014

6/1/20146 minutes, 42 seconds
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Low Nonfasting Triglycerides and Reduced All-Cause Mortality: A

Increased nonfasting plasma triglycerides indicating increase amounts of cholesterol in remnant liver proteins and are important risk factors for cardiovascular disease. In the May issue of Clinical Chemistry, researchers involved in the Copenhagen City Heart Study found that lower circulating triglyceride concentrations are associated with lower all-cause mortality.
5/28/201411 minutes, 40 seconds
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Interlaboratory Agreement of Insulin-like Growth Factor 1 Concentrations Measured by Mass Spectrometry

This is a podcast from Clinical Chemistry, sponsored by the Department of Laboratory Medicine at Boston Children's Hospital.
5/19/201412 minutes, 33 seconds
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Development of an Immunoassay for the Kidney-Specific Protein myo-

In well-resourced countries with a low incidence of tuberculosis, a major focus of TB control efforts is the detection and treatment of latent TB infection to prevent reactivation to active TB disease. This approach is particularly relevant for healthcare workers. Interferon Gamma Release Assays are used to detect the release of interferon from T cells stimulated by tuberculosis specific antigens. However, these tests differ in cost and complexity than the conventional tuberculin skin test.
5/12/201411 minutes, 27 seconds
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Interferon γ–Release Assays for Diagnosis of Latent Tuberculosis in Healthcare Workers in Low-Incidence Settings: Pros and Cons

In well-resourced countries with a low incidence of tuberculosis, a major focus of TB control efforts is the detection and treatment of latent TB infection to prevent reactivation to active TB disease. This approach is particularly relevant for healthcare workers. Interferon Gamma Release Assays are used to detect the release of interferon from T cells stimulated by tuberculosis specific antigens. However, these tests differ in cost and complexity than the conventional tuberculin skin test.
5/12/20149 minutes, 4 seconds
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Posttransplantation Bone Marrow Assessment by Quantifying Hematopoietic Cell–Derived mRNAs in Plasma Exosomes/Microvesicles

Recent studies have demonstrated that a variety of cells release exosomes or microvesicles into nearby biological fluids such as blood and saliva. During the exocytic process various proteins' messenger RNA and micro RNA are included in these exosomes.
5/7/20147 minutes, 1 second
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Sandwich Assay for Tacrolimus Using 2 Antitacrolimus Antibodies

Tacrolimus or FK506 is a macrocyclic lactone that is commonly used along with other immunosuppressant drugs to reduce graft rejection in organ transplantation by suppressing the immune system. Because of its narrow therapeutic window, it is critical to accurately monitor blood concentrations of this drug for optimal efficacy.
4/29/20147 minutes, 6 seconds
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Novel Uses for Platelet Function Testing in the Clinical Laboratory: Where Are We Now?

Platelet function testing has traditionally been used to diagnose inherited qualitative and quantitative defects in platelet function, such as Von Willebrand disease, but with the increased use of anti-platelet agents to prevent arterial thrombosis and the interest in identifying patients at risk for thrombosis despite anti-platelet therapy, the use of platelet function testing to monitory response to anti-platelet therapy, for example, aspirin, has become a hot topic.
4/21/201419 minutes, 28 seconds
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Publication and Reporting of Test Accuracy Studies Registered in ClinicalTrials.gov

Over the past several years, investigations have shown that many clinical studies remain unpublished and even among published studies the results are often presented selectively.
4/14/201411 minutes, 7 seconds
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Screening Method to Evaluate Point-of-Care Human Chorionic Gonadotropin (hCG) Devices for Susceptibility to the Hook Effect by hCG β Core Fragment: Evaluation of 11 Devices

Pregnancy tests such as the ones used to test urine in hospitals have been shown to give false-negative results in certain patients.
4/7/20148 minutes, 36 seconds
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Influence of PCR Reagents on DNA Polymerase Extension Rates Measured on Real-Time PCR Instruments

In the February 2014 issue of Clinical Chemistry, the influence of PCR reagents on DNA polymerase extension rates were studied by examining nucleotide incorporation with DNA dyes. We are joined by one of the authors of that study, Dr. Carl Wittwer. He is Professor of Pathology at the University of Utah Health Sciences Center and is also affiliated with ARUP Laboratories, BioFire Diagnostics, and is an Associate Editor of Clinical Chemistry.
4/2/201411 minutes, 58 seconds
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FullIssueSummary April2014

4/1/20149 minutes, 35 seconds
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Islet autoantibodies and type 1 diabetes: does the evidence support screening?

Type 1 diabetes is a chronic progressive autoimmune disorder with complex, polygenic susceptibility. Environmental factors which are poorly defined also contribute to the pathogenesis. This disease is characterized by lymphocyte infiltration into the islets of Langerhans in the pancreas, leading to inflammation and selective destruction of the insulin-producing beta cells resulting in hyperglycemia.
3/24/20146 minutes, 45 seconds
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Digital PCR as a Novel Technology and Its Potential Implications for Molecular Diagnostics

The latest incarnation of polymerase chain reaction, Digital PCR, takes three decades of development in enzyme chemistry and assay design and applies them with formidable precision and sensitivity.
3/17/201417 minutes, 28 seconds
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Diagnosis of Gestational Diabetes Mellitus: it is time for international consensus

Hemoglobin A1C is one of the analytes most commonly measured in clinical laboratories in patients with diabetes mellitus. Physicians use Hemoglobin A1C to monitor long-term glycemic control, adjust therapy, and predict complications of diabetes. It was recently added as a criterion for diagnosis of diabetes.
3/10/201417 minutes, 7 seconds
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Invention and Validation of an Automated Camera System That Uses Optical Character Recognition to Identify Patient Name Mislabeled Specimens

Although standardization of barcodes and label formats has lowered the number of mislabeled specimens in clinical laboratories, it remains a potential source of pre-analytical error. Published error rates of mislabeled specimens range up to as high as just over 1 percent.
3/6/201413 minutes, 23 seconds
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FullIssueSummary May2014

3/1/20147 minutes, 17 seconds
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FullIssueSummary March2014

3/1/201411 minutes, 16 seconds
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Diagnosis of Gestational Diabetes Mellitus: It Is Time for International Consensus.

Gestational diabetes mellitus is often defined as any degree of glucose intolerance during pregnancy. Fetal complications and adverse outcomes for both the fetus and the mother are important concerns in gestational diabetes.
2/25/201415 minutes, 54 seconds
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Genomic Test Validation for Incidental Findings

Acute kidney injury is an important health problem. Patients who develop acute kidney injury have increased in-hospital mortality, and if they do survive, they still suffer long-term increased morbidity and mortality. For that reason there has been great interest in the development of biomarkers that could identify kidney injury in its earliest stages, at a time when interventions might be more successful.
2/18/201414 minutes, 7 seconds
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Biomarkers for Acute Kidney Injury: Where Are We Today? Where Should We Go?

Acute kidney injury is an important health problem. Patients who develop acute kidney injury have increased in-hospital mortality, and if they do survive, they still suffer long-term increased morbidity and mortality. For that reason there has been great interest in the development of biomarkers that could identify kidney injury in its earliest stages, at a time when interventions might be more successful.
2/18/20148 minutes, 35 seconds
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As if Biomarker Discovery wasn’t Hard Enough

The discovery phase of proteomics is essential for the identification of suitable markers for exploration and validation of promising new clinical tests. But can researchers be certain if what they believe they are measuring is in fact what they are actually measuring?
2/4/20147 minutes, 32 seconds
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FullIssueSummary February2014

2/1/20149 minutes, 48 seconds
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Tumor Microenvironment–Released Peptides: Could They Form the Basis for an Early-Diagnosis Breast Cancer Test?

The January 2014 issue of Clinical Chemistry is devoted to the area of women's health. It includes a multi-center report on the application of measuring the circulating products of the proteolytic enzyme carboxypeptidase-N for the early detection of breast cancer. Accompanying that paper was an editorial by Eleftherios Diamandis on the tumor microenvironment and if released peptides could form the basis for early diagnosis breast cancer tests.
1/3/20147 minutes, 46 seconds
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What’s Different about Women’s Health?

Until recently, much of the research in medical literature used primarily male populations. Slowly we've come to appreciate the importance of studying female populations due to the differences in normal physiology as well as disease pathology between men and women.
1/3/20146 minutes, 26 seconds
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High-Sensitivity Cardiac Troponin Assays: Isn't It Time for Equality?

Women who present with acute coronary events are less frequently to be properly diagnosed and often have worse outcomes than men. Part of the problem may be that women are less apt to manifest increased biomarkers or often receive less aggressive guideline-mandated care. Women also have lower reference values for biomarkers of cardiac injury that are rarely taken into account.
1/3/201421 minutes, 2 seconds
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The Ethical Implications of Preimplantation Genetic Diagnosis

The development of in vitro fertilization in the 1970s has revolutionized the treatment of infertility. The ability to culture embryos has allowed for the development of the preimplantation genetic diagnosis. This involves removing a cell from the developing embryos for genetic testing before choosing one to implant. Just like prenatal diagnosis, it is used to screen for various genetic diseases before birth. For women of advanced maternal age or couples with known genetic mutations, the ability to screen of embryos free of certain genetic mutations is reassuring.
1/3/201411 minutes, 29 seconds
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Gestational Diabetes Mellitus

Gestational diabetes mellitus is becoming more common as the epidemic of obesity and type 2 diabetes continues. Newly proposed diagnostic criteria will, if adopted universally, further increase the prevalence of this condition.
12/10/201318 minutes, 32 seconds
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Diagnosing Diabetes Mellitus: Performance of Hemoglobin A1c Point-of-Care Instruments in General Practice Offices

In making the diagnosis of diabetes, there are several advantages using determinations of hemoglobin A1c rather than glucose measurements. These include the pre-analytics stability of hemoglobin A1c in the sample, a low within-subject biological variation, as well as being free from the dietary restrictions associated with measuring glucose.
12/3/201310 minutes, 36 seconds
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FullIssueSummary December2013

12/1/201310 minutes, 5 seconds
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Digital Droplet PCR for Rapid Quantification of Donor DNA in the Circulation of Transplant Recipients as a Potential Universal Biomarker of Graft Injury

Cell free DNA from transplanted organs in the circulation of transplant recipients is a potential biomarker of rejection. But most of these methods entail high costs, long turnaround times and the need for donor DNA.
11/18/20139 minutes, 24 seconds
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Multiplex Picodroplet Digital PCR to Detect KRAS

Multiplex digital PCR can be used for the sensitive detection of circulating tumor DNA with performance unachievable by other current molecular detection approaches.
11/11/20136 minutes, 42 seconds
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MicroRNA Signature Helps Distinguish Early from Late Biochemical Failure in Prostate Cancer

The elevation in the concentrations of prostate-specific antigen, or PSA, in blood after prostatectomy is the only available marker from monitoring relapse after surgery. This increase in PSA is sometimes called biochemical failure or biochemical relapse. PSA monitoring, however, cannot predict relapse at the time of surgery.
11/5/20138 minutes, 6 seconds
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FullIssueSummary November2013

11/1/201310 minutes, 45 seconds
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Bioinformatics: What the Clinical Laboratorian Needs to Know and Prepare For

New diagnostics technologies such as microarrays, next generation, or massively parallel sequencing, are generating an unprecedented amount of data. This requires a sophisticated knowledge of bioinformatics for proper storage, analysis, and mining of these very large data sets.
10/29/201313 minutes, 22 seconds
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Use of MALDI-TOF for diagnosis of microbial infections

The application of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry or MALDI-TOF MS, to microbial identification is revolutionizing clinical microbiology, providing rapid identification with minimal sample preparation and potential cost savings.
10/21/201313 minutes, 38 seconds
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ClinChem 201310 Rifai

And our guest today is Dr. Nader Rifai, Editor-in-Chief of Clinical Chemistry and Chair of the Clinical Chemistry Trainee Council, to announce the launch of a new program from AACC's Clinical Chemistry Trainee Council, the Question Bank. And Dr. Rifai, why was the Question Bank created?
10/14/20135 minutes, 42 seconds
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False Biomarker Discovery Due to Reactivity of a Commercial ELISA for CUZD1 with Cancer Antigen CA125

The discovery phase of proteomics is critical in the identification of suitable markers for exploration and validation of promising new clinical tests. But can laboratories be certain if what they believe they are measuring is, in fact, what they actually measuring?
10/4/201312 minutes, 6 seconds
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FullIssueSummary October2013

10/1/20139 minutes, 25 seconds
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Scanning for cancer genomic changes in plasma: towards an era of personalized blood-based tumor markers

The analysis of circulating cell-free tumor DNA has considerable potential for the detention and monitoring of cancers. Substantial effort has been made to identify cancer associated genetic changes that might be suitable for use as new tumor markers. However, only a few of these genetic markers have been translated into clinical use.
9/23/20137 minutes, 33 seconds
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Automation in the Clinical Microbiology Laboratory

The clinical microbiology laboratory is sometimes considered low-tech, particularly when compared to the high degree of automation found in the clinical chemistry laboratory. However, systems are emerging for the clinical microbiology laboratory with the potential to automate almost all areas of testing, including inoculation of primary culture plates, detection of growth on culture media, susceptibility testing, and extraction and detection of nucleic acids from clinical specimens.
9/16/201313 minutes, 16 seconds
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Automation in the Clinical Microbiology Laboratory

The clinical microbiology laboratory is sometimes considered low-tech, particularly when compared to the high degree of automation found in the clinical chemistry laboratory. However, systems are emerging for the clinical microbiology laboratory with the potential to automate almost all areas of testing, including inoculation of primary culture plates, detection of growth on culture media, susceptibility testing, and extraction and detection of nucleic acids from clinical specimens.
9/16/20130
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Point-of-Care and Over-the-Counter Qualitative Human Chorionic Gonadotropin (hCG) Devices Remain Susceptible to False-Negative Results Caused by Excess hCG β Core Fragment

For many taking a home pregnancy test can be upsetting, particularly if there is not complete confidence in the result. The November 2013 print issue of Clinical Chemistry published a paper from a group led by Dr. Ann Gronowski that reported false-negative results in point-of-care and over-the-counter qualitative hCG devices.
9/9/20137 minutes, 2 seconds
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Twenty-Eight Grams of Prevention Is Worth a Half Kilogram of Cure

Maintaining long-term stability of analytical procedures is an important responsibility for clinical laboratories. This process typically includes a comparison of current and new reagent lots through paired measurements of patient or control samples, with defined criteria for acceptance and rejection of the new lot.
9/3/201312 minutes, 21 seconds
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FullIssueSummary September2013

9/1/20139 minutes, 30 seconds
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A New Era in Prenatal Diagnosis: The Use of Cell-Free Fetal DNA in Maternal Circulation for Detection of Chromosomal Aneuploidies

Pregnant women identified as high risk based on the prenatal screen can then undergo invasive procedures such as amniocentesis to confirm the diagnosis. Unfortunately, a large number of women with unaffected pregnancies undergo invasive procedures, putting the fetus at unnecessary risk for miscarriage.
8/19/201322 minutes, 31 seconds
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Failure of Current Laboratory Protocols to Detect Lot-to-Lot Reagent Differences: Findings and Possible Solutions

Maintaining long-term stability of analytical procedures is an important undertaking for clinical laboratories. This process typically includes comparison of current and new reagent lots through paired measurement of patient and control samples, with defined criteria for acceptance and rejection of the new lot.
8/12/20139 minutes, 58 seconds
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ClinChem 201308 Korf

Personalized medicine implies that an individual's unique genetic makeup provides key information to guide prevention, diagnosis, and treatment of disease. It's highly probable that no one besides yourself has ever been born or ever will be again with your precise DNA sequence, unless you are an identical twin and even then maybe not.
8/6/201313 minutes, 46 seconds
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FullIssueSummary August2013

8/1/20139 minutes, 18 seconds
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FDA Oversight of Laboratory-Developed Tests: Is It Necessary, and How Would It Impact Clinical Laboratories?

The US Food and Drug Administration has long maintained its right to regulate LDTs; however until recently they've chosen to exercise discretion and have left this to the individual laboratories and other regulatory agencies. This is now in a state of change, and the FDA has stated that it would issue guidance on its oversight of Laboratory Developed Tests.
7/30/201310 minutes, 57 seconds
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FDA Oversight of Laboratory-Developed Tests: Is It Necessary, and How Would It Impact Clinical Laboratories?

The US Food and Drug Administration has long maintained its right to regulate LDTs; however until recently they've chosen to exercise discretion and have left this to the individual laboratories and other regulatory agencies. This is now in a state of change, and the FDA has stated that it would issue guidance on its oversight of Laboratory Developed Tests.
7/30/20130
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FDA Oversight of Laboratory-Developed Tests: Is It Necessary, and How Would It Impact Clinical Laboratories?

The US Food and Drug Administration has long maintained its right to regulate LDTs; however until recently they've chosen to exercise discretion and have left this to the individual laboratories and other regulatory agencies. This is now in a state of change, and the FDA has stated that it would issue guidance on its oversight of Laboratory Developed Tests.
7/30/20130
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Noninvasive Prenatal Methylomic Analysis by Genomewide Bisulfite Sequencing of Maternal Plasma DNA

Prenatal development involves a series of highly-organized genetic and epigenetic events. Abnormalities in the epigenetic control of developmental processes have been implicated in infertility, spontaneous abortion, intra-uterine growth abnormalities, and numerous post-natal consequences.
7/22/201320 minutes, 2 seconds
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Gaps in the Traceability Chain of Human Growth Hormone Measurements

Human growth hormone is an important peptide hormone that stimulates growth, cell reproduction, and cell regeneration. Measuring growth hormone in the blood is an important clinical assay, but may be problematic due to heterogeneity of the hormone that may arise from alternative splicing, different post-translational or modifications, oligomerization, formation of complexes, and proteolytic processing. Although standards for growth hormone are available, data from external quality assessment programs show large differences exist between measurement results obtained with different assays.
7/15/201310 minutes, 44 seconds
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Newborn Screening by Sequence and the Road Ahead

The collection and transport of dried blood spots has facilitated population screening of newborns worldwide. In the July 2013 issue of Clinical Chemistry, researchers from the Wadsworth Center at the New York State Department of Health in Albany described a convenient technique to extract DNA from these dried blood spots to further expand screening to nucleic acid testing.
7/8/201314 minutes, 8 seconds
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FullIssueSummary July2013

7/1/20139 minutes, 2 seconds
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Clinical Utility and Analytical Challenges in Measurement of Cerebrospinal Fluid Amyloid-β1–42 and τ Proteins as Alzheimer Disease Biomarkers

Alzheimer's disease is a complex progressive neurodegenerative disease leading to loss of memory and cognitive function, and pathologically characterized by amyloid plaques and tangles that are formed largely by fibular forms of beta-amyloid and hyper-phosphorylated tau proteins. During the past two decades, cumulative molecular and clinical studies have provided the basis for our understanding of the molecular characteristics and progressive pathologic features of these hallmarks.  
7/1/201322 minutes, 50 seconds
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Measurement of Thyroglobulin by Liquid Chromatography–Tandem Mass Spectrometry in Serum and Plasma in the Presence of Antithyroglobulin Autoantibodies

Measurement of serum thyroglobulin may be complicated by the presence of endogenous anti-thyroglobulin auto-antibodies which have the potential to interfere with immunoassays and cause false negative results.
6/17/20136 minutes, 38 seconds
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Cobalt and Chromium Measurement in Patients with Metal Hip Prostheses

Approximately 1 million metal-on-metal hip prostheses have been implanted worldwide to alleviate pain, restore hip function and improve overall quality of life.
6/10/201311 minutes, 31 seconds
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Beyond LDL-C in assessing cardiovascular risk: ApoB or LDL-P?

Low-density lipoprotein cholesterol, a key cardiovascular biomarker is recommended by National Cholesterol Education Program, Adult Treatment Panel Guidelines, for assessing cardiovascular disease risk and for monitoring lipid-lowering therapy; however, some workers believe that the number of circulating LDL particles rather than LDL cholesterol is a strong indicator of future cardiovascular disease events.
6/4/20138 minutes, 9 seconds
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FullIssueSummary June2013

6/1/20139 minutes, 28 seconds
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Are Biomarkers the Answer to the Heart Failure Readmissions Problem?

Readmissions after hospitalization for heart failure are an increasingly important problem, with a significant number of patients being re-hospitalized within 30 days of discharge.
5/28/20138 minutes, 21 seconds
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Vitamin D and Cancer: Can We Believe the Evidence from Observational Studies?

Chemicals in tobacco smoke may influence vitamin D metabolism and function, and vitamin D itself may modify the carcinogenity of tobacco smoke.
5/20/201313 minutes, 59 seconds
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Circulating MicroRNAs: What Is Their Relevance?

A paper in the May 2013 issue of Clinical Chemistry found that circulating microRNAs were deregulated in severe obesity, and the lead author of that study Dr. Francisco José Ortega from Spain joined us earlier for a separate podcast. That paper was accompanied by an editorial addressing the relevance of circulating microRNAs in plasma. The lead author of that commentary, Dr. Catriona Hilton from the Oxford Centre for Diabetes, Endocrinology, and Metabolism in Oxford, England, joins us today in this podcast.
5/13/20138 minutes, 25 seconds
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Molecular detection of human H7N9 influenza A virus causing outbreaks in China

Recently there has been a large amount of attention on a novel human influenza A virus, the H7N9 strain, that as of May 2013, has so far sickened over 131 people and killed 31 in the People's Republic of China. Because of the potential for pandemic spread, there is a great need for rapid and accurate test for the detection of the virus.
5/9/20136 minutes, 46 seconds
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Targeting the Circulating MicroRNA Signature of Obesity

Genomics has the potential to provide important insights into the pathogenesis of obesity. A paper in the May 2013 issue of Clinical Chemistry found that circulating microRNAs were deregulated in severe obesity.
5/6/201312 minutes, 19 seconds
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FullIssueSummary May2013

5/1/20137 minutes, 51 seconds
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The Economic Potential of Pharmacogenomics

enomic research has been widely expected to transform medicine, but progress is not as rapid as some have expected. In the April 2013 issue of Clinical Chemistry, Ramy Arnaout and his colleagues published a paper regarding the cost and the pace of advances in pharmacogenomics.
4/29/20136 minutes, 11 seconds
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The Human Gut Microbiome and Body Metabolism: Implications for Obesity and Diabetes

Obesity, metabolic syndrome, and diabetes are major public health challenges. Recently, interest has surged regarding the possible role of intestinal microbes as potential contributors to the increased prevalence of these three disorders.
4/22/201323 minutes, 27 seconds
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Q&A on Pheochromocytoma

Pheochromocytomas are tumors of the adrenal gland that secrete catecholamine. Closely related tumors called extraadrenal paragangliomas can arise at extraadrenal sites. Catecholamine secretion from these tumors causes headache, perspiration, palpitations and hypertension. If not recognized and treated, pheochromocytoma and extraadrenal paraganglioma can lead to arrhythmias, myocardial infarction, stroke and death.
4/15/201320 minutes, 51 seconds
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Mobile device for disease diagnosis and data tracking in resource-limited settings

In resource-limited settings, both access to laboratory diagnostics and to patients' health records can be challenging. In the April 2013 issue of Clinical Chemistry, Dr. Samuel Sia and his colleagues described a low-cost mobile device that combines cellphone and satellite communication technologies along with fluid miniaturization techniques for performing all of the functions of an enzyme-linked immunoassay.
4/10/201315 minutes, 45 seconds
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MicroRNAs in Idiopathic Childhood Nephrotic Syndrome

MicroRNAs are present in body fluids and have the potential to serve as disease biomarkers. A study in the April 2013 issue of Clinical Chemistry explored the clinical value of microRNAs in serum and urine as biomarkers for idiopathic childhood nephrotic syndrome.  This paper was accompanied by an editorial by Johan Lorenzen and Thomas Thum, both from the Hannover Medical School in Hannover, Germany. They both join us today in this podcast.
4/2/20136 minutes
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Predicting the Cost and Pace of Pharmacogenomic Advances: An Evidence-Based Study

Genomic research had been widely expected to transform medicine, but progress has been slower than some have expected. To critics delays represent broken promises and/or a sign that at least some of the money spent on genomic research might have been better spent elsewhere.
4/1/20139 minutes, 6 seconds
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FullIssueSummary April2013

4/1/20138 minutes, 51 seconds
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Communication of Scientific Information: Is It Time to Reassess?

4/1/201313 minutes, 21 seconds
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Male Infertility and Microchips

In the March 2013 issue of Clinical Chemistry, Dr. Yuan Chen and colleagues of the National Taiwan University demonstrated a new miniaturized device that performs most of the key semen analyses that predict male infertility.
3/25/20137 minutes, 45 seconds
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Cannabis Effects on Driving Skills

Cannabis is the most prevalent illicit drug identified in impaired drivers. The effects cannabis has on driving continue to be debated, making legislation and prosecution difficult.
3/18/201322 minutes, 19 seconds
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ClinChem 201305 Nordestgaard

For decades, vitamin D deficiency was thought a thing of the distant past, but recent evidence has associated low vitamin D levels with a number of diseases and conditions. Tobacco smoke chemicals may influence vitamin D metabolism and function, and vitamin D itself may modify the carcinogenicity of tobacco smoke.
3/18/201310 minutes, 31 seconds
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More Data, Please!

"More Data, Please!" That's the provocative title of Dr. Keith Baggerly's editorial in the March 2013 issue of Clinical Chemistry. He was commenting on an article appearing in the previous month of the journal by Kenneth Witwer who joined us in a podcast earlier this year, regarding the state of data reporting in microRNA studies.
3/11/201317 minutes, 41 seconds
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MALDI-TOF Mass Spectrometry: Transformative Proteomics for Clinical Microbiology

Mass spectrometry has revolutionized many areas of clinical chemistry, but this technology is not just limited to chemistry. Introduction of MALDI-TOF mass spectrometry into the clinical microbiology laboratory has markedly altered workflow allowing bacterial and fungal colonies to be accurately, rapidly, and inexpensively identified.
3/4/20134 minutes, 49 seconds
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FullIssueSummary March2013

3/1/20137 minutes, 41 seconds
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Is Early Detection of Cancer with Circulating Biomarkers Feasible?

Early cancer detection before metastasis in asymptomatic patients is one of the primary objectives of cancer research initiatives. Early detection generally means more opportunities for intervention that ultimately lead to improvements in patient outcomes.
2/25/201314 minutes, 24 seconds
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MicroRNA Analysis: Is It Ready for Prime Time?

2/19/20139 minutes
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External Quality Assessment of Point-of-Care Methods: Model For Combined Assessment of Method Bias and Single-Participant Performance by the Use of Native Patient Samples and Noncommutable Control Material

2/19/201310 minutes, 44 seconds
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Data Submission and Quality in Microarray-Based MicroRNA Profiling

2/11/201311 minutes, 57 seconds
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Commercial Immunoassays in Biomarkers Studies: Researchers Beware!

2/11/20138 minutes, 28 seconds
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Comparing Multiple Measures of Glycemia: How to Transition from Biomarker to Diagnostic Test?

2/4/201318 minutes, 46 seconds
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FullIssueSummary Feb2013

2/1/20137 minutes, 30 seconds
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The Riddle of Protein Diagnostics: Future Bleak or Bright?

1/28/20138 minutes, 54 seconds
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Conquering Cancer in Our Lifetime: New Diagnostic and Therapuetic Trends

1/22/20139 minutes, 27 seconds
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Plasma-derived Tumor DNA analysis at whole genome resolution;

1/14/201312 minutes, 20 seconds
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Validating Serum Markers for Monitoring of Cancer

1/7/20135 minutes, 8 seconds
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Cancer Genome Scanning in Plasma: Detection of Tumor-Associated Copy Number Aberrations, Single-Nucleotide Variants, and Tumoral Heterogeneity by Massively Parallel Sequencing

1/3/20135 minutes, 8 seconds
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Design of Tumor Biomarker-Monitoring Trials: A Proposal by the European Group on Tumor Markers

1/3/201311 minutes, 38 seconds
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FullIssueSummary Jan2013

1/1/201312 minutes, 30 seconds
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Associations of Alternative Markers of Glycemia with Hemoglobin A1c and Fasting Glucose

12/27/20124 minutes, 15 seconds
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Quantitative PCR Analysis of DNA, RNAs, and Proteins in the Same Single Cell

12/17/201212 minutes, 41 seconds
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The Evolving Threat of Influenza Viruses of Animal Origin and the Challenges in Developing Appropriate Diagnostics;

12/17/20128 minutes, 26 seconds
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Performance Criteria for Testosterone Measurements Based on Biological Variation in Adult Males: Recommendations from the Partnership for the Accurate Testing of Hormones

12/10/20129 minutes, 43 seconds
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Medicine Unplugged: The Future of Laboratory Medicine

12/6/20128 minutes, 33 seconds
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Medicine Unplugged: The Future of Laboratory Medicine

12/6/20128 minutes, 33 seconds
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Longitudinal Studies of Cardiac Troponin I in a Large Cohort of Healthy Children

12/3/201211 minutes, 5 seconds
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FullIssueSummary Dec2012

12/1/20126 minutes, 59 seconds
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Determination of 19 Cardiac Troponin I and T Assay 99th Percentile Values from a Common Presumably Healthy Population

11/27/201214 minutes, 51 seconds
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On-Site Test for Cannabinoids in Oral Fluid

11/20/201216 minutes, 9 seconds
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ClinChem FullIssueSummary Nov2012

11/14/20125 minutes, 33 seconds
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Ectopic Pregnancy

11/13/20126 minutes, 29 seconds
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Exome and Whole-Genome Sequencing as Clinical Tests: A Transformative Practice in Molecular Diagnostics

11/7/201210 minutes, 29 seconds
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More Reliable On-Site Detection of Cannabis in Oral Fluid

10/31/20126 minutes, 37 seconds
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Room for Improvement in National Academy of Clinical Biochemistry Laboratory Medicine Practice Guidelines

10/22/20125 minutes, 35 seconds
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Applicability of the AGREE II Instrument in Evaluating the Development Process and Quality of Current National Academy of Clinical Biochemistry Guidelines

10/22/20128 minutes, 25 seconds
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The Human Salivary RNA Transcriptome Revealed by Massively Parallel Sequencing

10/15/201215 minutes, 11 seconds
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Noninvasive Prenatal Diagnosis of Monogenic Diseases by Targeted Massively Parallel Sequencing of Maternal Plasma: Application to β-Thalassemia

10/15/20125 minutes, 44 seconds
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Caution Is Needed in the Interpretation of Added Value of Biomarkers Analyzed in Matched Case Control Studies

10/8/201211 minutes, 51 seconds
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Whole-Blood Thrombin Generation Monitored with a Calibrated Automated Thrombogram-Based Assay

10/8/20127 minutes, 25 seconds
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Interpreting Cardiac Troponin Results from High-Sensitivity Assays in Chronic Kidney Disease without Acute Coronary Syndrome

10/5/20129 minutes, 3 seconds
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Thyroid Function during Pregnancy: Who and How Should We Screen?

10/5/201222 minutes, 40 seconds
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Circulating Human Hepcidin-25 Concentrations Display a Diurnal Rhythm, Increase with Prolonged Fasting, and Are Reduced by Growth Hormone Administration

9/28/20127 minutes, 53 seconds
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Antibiotic Resistance: How Serious Is the Problem, and What Can Be Done?

9/28/201212 minutes, 23 seconds
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Establishing Pediatric Reference Intervals: A Challenging Task

9/18/20128 minutes
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A Unique Approach to Business Strategy as a Means to Enable Change in Global Healthcare: A Case Study

9/18/201210 minutes, 28 seconds
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The Obesity Epidemic

8/31/201211 minutes, 31 seconds
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Diagnostic Accuracy of Point-of-Care Fecal Calprotectin and Immunochemical Occult Blood Tests for Diagnosis of Organic Bowel Disease in Primary Care: The Cost-Effectiveness of a Decision Rule for Abdominal Complaints in Primary Care (CEDAR) Study

8/31/20127 minutes, 9 seconds
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Newer Fecal Tests: Opportunities for Professionals in Laboratory Medicine

8/31/201212 minutes, 57 seconds
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Altering the Landscape for Women in Clinical Chemistry: Perspectives from Multigenerational Leaders

8/31/20126 minutes, 43 seconds
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Clinician's Perspective: Allan Jaffe

8/23/201214 minutes, 38 seconds
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Elementary, My Dear Doctor Watson

8/23/201210 minutes, 30 seconds
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Patents and Laboratory Medicine: What Is Required to Turn an Unpatenable Natural Phenomenon or Law of Nature into a Patenable Invention?

8/14/201210 minutes, 39 seconds
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Closing the Gaps in Pediatric Laboratory Reference Intervals: A CALIPER Database of 40 Biochemical Markers in a Healthy and Multiethnic Population of Children

8/6/201219 minutes, 16 seconds
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Catch Me If You Can: Isolating Circulating Tumor Cells from Flowing Blood

8/6/20126 minutes, 33 seconds
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Genetic Susceptibility for Coronary Heart Disease and Type 2 Diabetes Complications

8/6/20127 minutes, 45 seconds
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Inappropriate Requesting of Glycated Hemoglobin (Hb A1c) Is Widespread: Assessment of Prevalence, Impact of National Guidance, and Practice-to-Practice Variability

8/6/201235 minutes, 22 seconds
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Fluorescence Sensor for the Quantification of Unbound Bilirubin Concentrations

7/11/201210 minutes, 38 seconds
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Refining Noninvasive Prenatal Diagnosis with Single-Molecule Next-Generation Sequencing

7/11/20125 minutes, 9 seconds
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Understanding the Mechanisms That Link Alcohol and Lower Risk of Coronary Heart Disease

7/11/201224 minutes, 12 seconds
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Microtube Device for Selectin-Mediated Capture of Viable Circulating Tumor Cells from Blood

7/11/20126 minutes, 30 seconds
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Point/Counterpoint: Standardization of Cardiac Troponin I

7/3/201222 minutes, 45 seconds
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Discerning Trends in Multiplex Immunoassay Technology with Potential for Resource-Limited Settings

7/3/201211 minutes, 14 seconds
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Interview with Leo L.M. Poon on Molecular Detection of a Novel Human Influenza H1N1

Leo L.M. Poon, K.H. Chan, G.J. Smith, C.S.W. Leung, Y. Guan, K.Y. Yuen, and J.S.M. Peiris Molecular Detection of a Novel Human Influenza (H1N1) of Pandemic Potential by Conventional and Real-Time Quantitative RT-PCR Assays Clin Chem published May 13, 2009
6/20/20125 minutes, 24 seconds
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Interview with Larry D. Bowers on the International Anti-Doping System

6/20/201217 minutes, 20 seconds
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High-Sensitivity Troponin Assays, Interview with Dr. Allan S. Jaffe

6/20/201218 minutes, 32 seconds
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Interview with Graham H. Beastall on the Reinvention of Vitamin D and Its Implications for Clinical Chemistry

6/20/201212 minutes, 19 seconds
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Lily Robinson and the Art of Secret Poisoning

6/20/20129 minutes, 13 seconds
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Inspiring Minds, Gerald Cooper

6/20/20127 minutes, 48 seconds
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Interview with David B. Sacks on Tight Glucose Control in Intensive Care Units

6/20/201218 minutes, 31 seconds
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Interview with Karl V. Voelkerding on Next-Generation Sequencing

6/20/201214 minutes, 47 seconds
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Interview with Fred S. Apple on the Role of Sensitive Cardiac Troponin I Assay for Early Diagnosis of Myocardial Infarction

6/20/201217 minutes, 39 seconds
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Inspiring Minds: Jack Ladenson

Clinical Chemistry 54: 613-614, 2008
6/20/20126 minutes, 46 seconds
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Interview with Samia Mora and Børge G. Nordestgaard on Comparison of LDL

6/20/201217 minutes, 28 seconds
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Interview with Dr. Allan Sniderman on Use of Apolipoprotein B

6/20/201212 minutes, 12 seconds
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Interview with Carl T. Wittwer and Stephen A. Bustin on the MIQE Guidelines

6/20/201219 minutes, 29 seconds
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Interview with Dr. Paul Ridker: Lead Investigator of the JUPITER Study

http://www.clinchem.org/
6/20/201211 minutes, 44 seconds
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Lily Robinson and the Mount Vernon Affair

6/20/20129 minutes, 32 seconds
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Interview with Steven J. Soldin on Free Thyroid Hormones Measured by Tandem Mass Spectrometry and Immunoassay with Thyroid-Stimulating Hormone across 4 Patient Populations

6/20/20129 minutes, 59 seconds
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Interview with Dr. Paul Ridker: Lead Investigator of the JUPITER Study

6/20/201211 minutes, 44 seconds
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IFCC Working Group for Standardization of Thyroid Function Tests

6/19/201223 minutes, 13 seconds
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Proteomics Special Issue

6/19/201227 minutes, 13 seconds
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New Ultraperformance-Tandem Mass Spectrometry Oral-Fluid Assay for 29 Illicit Drugs and Medications

6/19/201224 minutes, 24 seconds
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The D-lemma: To Screen or Not to Screen for 25-Hydroxyvitamin D Concentrations

6/19/201218 minutes, 11 seconds
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National Academy of Clinical Biochemistry Laboratory Medicine Practice Guidelines for Use of Tumor Markers in Liver, Bladder, Cervical, and Gastric Cancers

6/19/201222 minutes, 27 seconds
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Inspiring Minds, Carl Wittwer

6/19/20128 minutes, 56 seconds
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A 25-Year Prospective Study of Plasma Adiponectin and Leptin Concentrations and Prostate Cancer Risk and Survival

6/19/201224 minutes, 21 seconds
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Interview with Hans Lilja and Andrew J. Vickers on Cutpoints in Clinical Chemistry

6/19/201217 minutes, 4 seconds
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Enrichment and Detection of Rare Alleles by Means of Snapback Primers and Rapid-Cycle PCR

6/19/201210 minutes, 22 seconds
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Adventures in Clinical Chemistry and Proteomics: A Personal Account

6/19/201211 minutes, 1 second
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Interactive Modeling for Ongoing Utility of Pharmacogenetic Diagnostic Testing: Application for Warfarin Therapy

6/19/20124 minutes, 31 seconds
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David E. Bruns on the Stabilization of Glucose in Blood Specimens

http://www.clinchem.org/cgi/content/full/55/5/850
6/19/201213 minutes, 14 seconds
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Interview with James Versalovic on Metagenomic Pyrosequencing and Microbial Identification

6/19/201214 minutes, 48 seconds
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Serum Creatinine and Glomerular Filtration Rate: Perception and Reality

6/19/201214 minutes, 52 seconds
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Six of Eight Hemoglobin A1c Point-of-Care Instruments Do Not Meet the General Accepted Analytical Performance Criteria

6/19/201211 minutes, 49 seconds
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Interview with Marilyn A. Huestis on Urinary Excretion of Buprenorphine in Pregnant Women

6/19/20129 minutes, 40 seconds
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Detection of Biological Agents Used for Terrorism: Are We Ready?

6/19/201217 minutes, 51 seconds
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Implications of Elevated C-reactive Protein for Cardiovascular Risk Stratification in Black and White Men and Women in the United States

6/19/201213 minutes, 5 seconds
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Interview with Donald W. Jacobsen on Total Plasma Homocysteine

6/19/201214 minutes, 1 second
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Design and Optimization of Reverse-Transcription Quantitative PCR Experiments

6/19/201212 minutes, 59 seconds
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γ′ Fibrinogen: Evaluation of a New Assay for Study of Associations with Cardiovascular Disease

6/19/201221 minutes, 13 seconds
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MicroRNAs as Novel Myocardial Biomarkers

6/19/20125 minutes, 47 seconds
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Interview with George G. Klee and Stephen R. Master on Measurement and Quality Control in Protein Microassays

6/19/201212 minutes, 24 seconds
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Interview with Michael J. Bennett on National Academy of Clinical Biochemistry Laboratory Medicine Practice Guidelines

6/19/201210 minutes, 21 seconds
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Reference Measurement Procedure for Total Glycerides by Isotope Dilution GC-MS

6/14/20128 minutes, 51 seconds
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Personalized Cancer Genomics: The Road Map to Clinical Implementation

6/14/201212 minutes, 39 seconds
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Single Molecule Sequencing of Free DNA from Maternal Plasma for Noninvasive Trisomy 21 Detection

6/14/20124 minutes, 32 seconds
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Direct-to-Consumer Genetic Testing: Reliable or Risky?

5/5/201210 minutes, 19 seconds
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How Well Are We Training the Next Generation of Clinical Pathologists and Clinical Laboratory Directors? A Global Perspective

4/26/201214 minutes, 3 seconds
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Fragile X Mental Retardation 1 (FMR1) Intron 1 Methylation in Blood Predicts Verbal Cognitive Impairment in Female Carriers of Expanded FMR1 Alleles: Evidence from a Pilot Study

4/26/201212 minutes, 56 seconds
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Accuracy of 6 Routine 25-Hydroxyvitamin D Assays: Influence of Vitamin D Binding Protein Concentration

4/26/20127 minutes, 17 seconds
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Newborn Screening for Metabolic Disorders: How Are We Doing, and Where Are We Going?

3/23/201210 minutes, 25 seconds
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Searching for New Biomarkers of Renal Diseases through Proteomics

3/23/201218 minutes, 25 seconds
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Exhaled Endogenous Particles Contain Lung Proteins

3/23/20125 minutes, 52 seconds
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Detection of Myocardial Infarction—Is It All Troponin?

3/23/201219 minutes, 31 seconds
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Diagnostic Accuracy of Plasma Glial Fibrillary Acidic Protein for Differentiating Intracerebral Hemorrhage and Cerebral Ischemia in Patients with Symptoms of Acute Stroke

3/8/20127 minutes, 55 seconds
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The Challenges and Concerns Companies Face Pertaining to the US Food and Drug Administration 510(k) Process for Cardiac Biomarkers

3/8/20127 minutes, 35 seconds
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Absolute and Relative Kinetic Changes of High-Sensitivity Cardiac Troponin T in Acute Coronary Syndrome and in Patients with Increased Troponin in the Absence of Acute Coronary Syndrome

3/8/201212 minutes, 24 seconds
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Within Sight of a Rational Pipeline for Development of Protein Diagnostics

3/8/20124 minutes, 13 seconds
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Growth Differentiation Factor 15: A Canary in a Coal Mine?

2/22/201212 minutes, 18 seconds
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A Novel Technology for Measuring Cumulative Cardiac Biomarker Exposure over Time: What Happened When We Weren't Looking?

2/22/201210 minutes, 43 seconds
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Impact of Biomarkers, Proteomics, and Genomics in Cardiovascular Disease

2/22/20127 minutes, 36 seconds
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Association of Growth Differentiation Factor-15 with Coronary Atherosclerosis and Mortality in a Young, Multiethnic Population: Observations from the Dallas Heart Study

1/20/20127 minutes, 43 seconds
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Markers of Plaque Instability in the Early Diagnosis and Risk Stratification of Acute Myocardial Infarction

1/20/20127 minutes, 41 seconds
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Hepcidin in Human Iron Disorders: Diagnostic Implications

1/20/201210 minutes, 30 seconds
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Improved Monitoring of Differences in Serial Laboratory Results

1/5/201210 minutes, 17 seconds
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An About-Face for C-Reactive Protein?

11/28/201114 minutes, 57 seconds
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Investigating Interferences of a Whole-Blood Point-of-Care Creatinine Analyzer: Comparison to Plasma Enzymatic and Definitive Creatinine Methods in an Acute-Care Setting

11/28/201110 minutes, 46 seconds
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Quantification of 1a,25-Dihydroxy Vitamin D by Immunoextraction and Liquid Chromatography-Tandem Mass Spectrometry

11/28/201113 minutes, 5 seconds
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Preanalytical Errors Introduced by Sample-Transportation Systems: A Means to Assess Them

11/8/20116 minutes, 58 seconds
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Determination of Hemolysis Thresholds by the Use of Data Loggers

11/8/20118 minutes, 31 seconds
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The Quest for Clean Competition in Sports: Are the Testers Catching the Dopers?

10/7/201117 minutes, 7 seconds
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I Detected My Cancer with My Smart Phone

10/7/20119 minutes, 17 seconds
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Oral Fluid Cannabinoids in Chronic, Daily Cannabis Smokers during Sustained, Monitored Abstinence

9/28/201118 minutes, 48 seconds
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Between-Method Differences in Prostate-Specific Antigen Assays Affect Prostate Cancer Risk Prediction by Nomograms

9/20/201111 minutes, 23 seconds
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Optimal Detection of Fetal Chromosomal Abnormalities by Massively Parallel DNA Sequencing of Cell-Free Fetal DNA from Maternal Blood

7/25/201110 minutes, 29 seconds
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Diagnostic Potential of Saliva: Current State and Future Applications

6/20/201112 minutes, 6 seconds
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Diabetes: Advances and Controversies

6/20/201117 minutes, 24 seconds
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Building Multidimensional Biomarker Views of Type 2 Diabetes on the Basis of Protein Microheterogeneity

5/26/201113 minutes, 6 seconds
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'Clinical Chemistry' Trainee Council: A New Journal Initiative

5/16/20115 minutes, 16 seconds
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Point-of-Care Testing for Hb A1c in the Management of Diabetes: A Systematic Review and Metaanalysis

5/11/201131 minutes, 9 seconds
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The Use of Human Tissues in Research: What Do We Owe the Research Subjects?

5/11/201117 minutes, 13 seconds
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Non–HDL Cholesterol Shows Improved Accuracy for Cardiovascular Risk Score Classification Compared to Direct or Calculated LDL Cholesterol in a Dyslipidemic Population

5/11/201115 minutes, 32 seconds
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The Journey to Regulation of Protein-Based Multiplex Quantitative Assays

4/22/201120 minutes, 40 seconds
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High-Sensitivity Cardiac Troponin for Screening Large Populations of Healthy People: Is There Risk?

4/21/201117 minutes, 37 seconds
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Increasing Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) Throughput by Mass Tagging: A Sample-Multiplexed High-Throughput Assay for 25-Hydroxyvitamin D2 and D3

3/24/201115 minutes, 28 seconds
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Concordance Study of 3 Direct-to-Consumer Genetic-Testing Services

3/18/201111 minutes, 46 seconds
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Clinical Chemisty Podcast

3/18/201118 minutes, 20 seconds
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Potential for Electronic Health Records and Online Social Networking to Redefine Medical Research

3/4/201115 minutes, 9 seconds
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International Year of Chemistry 2011: Clinical Chemistry Celebrates

2/4/201115 minutes, 17 seconds
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Inspiring Minds: Larry Kricka

12/16/20109 minutes, 50 seconds
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Lipoprotein(a) and Risk of Type 2 Diabetes

12/8/201036 minutes, 8 seconds
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Determination of Total Homocysteine, Methylmalonic Acid, and 2-Methylcitric Acid in Dried Blood Spots by Tandem Mass Spectrometry

12/1/201012 minutes, 38 seconds
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Reporting of Estimated Glucose with Hemoglobin A1c

12/1/201025 minutes, 1 second
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A Multiplex Immunoassay Using the Guthrie Specimen to Detect T-Cell Deficiencies Including Severe Combined Immunodeficiency Disease

11/17/201026 minutes, 55 seconds
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Patient Misidentifications Caused By Errors in Standard Bar Code Technology*

10/29/201023 minutes, 28 seconds
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Gene Signatures for Cancer Therapy

9/15/20108 minutes, 39 seconds
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Clinical Impact of Reporting Estimated Glomerular Filtration Rates

9/15/201012 minutes, 36 seconds
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Analysis of the Size Distributions of Fetal and Maternal Cell-Free DNA by Paired-End Sequencing

9/15/20103 minutes, 29 seconds
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Direct-to-Consumer Genotyping

9/8/20107 minutes, 56 seconds
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A Critique of the Hypothesis, and a Defense of the Question, as a Framework for Experimentation

8/26/20107 minutes, 33 seconds
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Disposition of Cannabinoids in Oral Fluid after Controlled Around-the-Clock Oral THC Administration

8/26/201028 minutes, 34 seconds
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Microtechnology in the Clinical Laboratory

8/26/201015 minutes, 5 seconds
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Few Point-of-Care Hemoglobin A1c Assay Methods Meet Clinical Needs

8/26/201013 minutes, 50 seconds
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Quantitative 1-Step DNA Methylation Analysis with Native Genomic DNA as Template

8/26/20106 minutes, 20 seconds
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Pre-β1 HDL and Ischemic Heart Disease

6/16/201012 minutes, 25 seconds
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Troponin I, the Story

5/27/201012 minutes, 5 seconds
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Commentary on the 2009 Canadian Guidelines for the Diagnosis and Treatment of Dyslipidemia and Prevention of Cardiovascular Disease in Adults*

5/27/20105 minutes, 50 seconds
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Combining Biochemical and Ultrasonographic Markers in Predicting Preeclampsia: A Systematic Review

http://www.clinchem.org/cgi/content/abstract/56/3/361
4/6/20100
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Combining Biochemical and Ultrasonographic Markers in Predicting Preeclampsia: A Systematic Review

4/6/201015 minutes, 42 seconds
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Implementation of a Closed-Loop Reporting System for Critical Values and Clinical Communication in Compliance with Goals of The Joint Commission

http://www.clinchem.org/cgi/content/abstract/56/3/417
4/6/20109 minutes, 8 seconds
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Clinical Chemistry Podcast

3/29/201013 minutes, 32 seconds
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Protein Microarrays for Personalized Medicine

3/29/20108 minutes, 3 seconds
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High-Sensitivity Cardiac Troponin: Hype, Help, and Reality

3/8/201012 minutes, 18 seconds
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Inspiring Minds - Carl Burtis

3/1/20096 minutes, 56 seconds